Flammulina velutiper suitable for industrial cultivation and molecular identification method of Flammulina velutiper

The technology of Flammulina velutipes and Wanjin is applied in the field of Flammulina velutipes Nongwanjin No. 8 and its molecular identification.

Active Publication Date: 2020-11-27
FUJIAN AGRI & FORESTRY UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention will solve the technical problems of low yield per unit area, irregular fruiting, and yellowish color of the species of Flammulina velutipes used in the existing production, thereby providing a plant with a large number of buds. , the mushroom body is neat, the mushroom body is white, the stalk tastes crisp, the quality is high, and the yield is high. Flammulina velutipes

Method used

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  • Flammulina velutiper suitable for industrial cultivation and molecular identification method of Flammulina velutiper
  • Flammulina velutiper suitable for industrial cultivation and molecular identification method of Flammulina velutiper
  • Flammulina velutiper suitable for industrial cultivation and molecular identification method of Flammulina velutiper

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Select Flammulina velutipes 'F0321' and 'F0323' that are mainly planted as hybrid parents, select 20 single-spore strains of parent 'F0321', and 20 single-spore strains of 'F0323' grow faster, and the aerial hyphae are more developed The monospore strains were paired according to the full combination method, and a total of 60 combinations were prepared, and the number of hybrids containing lock joints was 47 after microscopic examination. After the initial fruiting test, 20 hybrids capable of fruiting were selected and entered. Double screen. After re-screening, 14 hybrids produced fruit normally, and 13 hybrids had yields higher than the control strain 'Nongjin No. 3', but only 3 strains had yields higher than 400g / bottle, of which Flv0012 The comprehensive traits are good, and the expected breeding goal is achieved. The hybrid strain is named "Nongwanjin No. 8" and will be used for subsequent batch tests.

[0016] The differences between the 'Nongwanjin...

Embodiment 2

[0022] Example 2: Using SV detection technology to compare the DNA differences between this strain and its parent.

[0023] Test materials: 5 strains of Flammulina velutipes, among which the strain to be tested is 'Nong Wan Jin No. 8', the control strains F0321, F0323, F0303 (Nong Jin No. 3), and Nong Jin No. 6.

[0024]Inspection method: According to the genome sequencing data, Kmer subtractive assembly technology is used to obtain differential fragments (SV), and several SV sequences are selected accordingly to design specific primers to amplify the samples to be tested. (For references, see Alkan, C., Coe, B. P. & Eichler, E. E. Genome structural variation discovery and genotyping. Nature reviews. Genetics, 2011 (12): 363–376; - Modernization of Traditional Chinese Medicine, 21(4): 764-774.). SV test primer sequences were synthesized by Qingke Biotechnology Co., Ltd.

[0025] The mycelium activation, cultivation, genomic DNA extraction and electrophoresis detection of the...

Embodiment 3

[0070] Example 3: 'Nongwanjin No. 8' has carried out multiple yield stability tests. The results show that 'Nongwanjin No. 8' meets the requirements of industrial cultivation, and its yield is significantly higher than that of the parent F0303 (Nongjin No. 3).

[0071] Stable production test strain: 'Nongjin 3' (Flv0303).

[0072] Test location: Fujian Wanchen Biotechnology Co., Ltd.

[0073] Test arrangement: uniform production of strains, factory cultivation at the test site according to local production management methods, bottle cultivation, and multi-zone mobile bed frame cultivation (plastic bottle, 0.43kg (dry material) / bottle).

[0074] Test results: During the experimental seed production period, the mycelium of 'Nongwanjin No. 8' was thicker in PDA, and the optimum growth temperature was 20 ℃. The cultivation results of 'Nongwanjin No.8' are as follows: figure 1 shown.

[0075] Cultivation findings: (1) The time from inoculation to harvest, 'Nongwanjin 8' and the...

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Abstract

The invention discloses a Flammulina velutiper strain Nongwanjin No.8 which is preserved in China General Microbiological Culture Collection Center, and belongs to Flammulina velutipes. The Flammulinavelutiper strain Nongwanjin No.8 has excellent Flammulina velutiper strain's quality characteristics of pure white mushroom body, slightly large pileus, slightly thick stipe, strong and firm mushroomstems, less villus at root. The Flammulina velutiper strain overcomes defects of larger and thinner pileus, softer stipe, poorer commodity appearance and the like of a parent F0321 and large difficulty in cultivation management, high probability of producing fungus films and terminal buds and the like of another parent F0323, the yield is increased by 28.6% when compared with that of a strain Nongjin No.3 (Flv0303), and the strain has good commodity appearance, meets demands of the market, has excellent overall performance, is suitable for industrial production and has good popularization prospects.

Description

technical field [0001] The invention relates to a strain of Flammulina velutipes strain. Specifically, it relates to Flammulina velutipes Nongwanjin No. 8 suitable for industrial cultivation and a molecular identification method thereof. Background technique [0002] Flammulina velutipes [ Flammulina velutiper (Fr.) Sing] belongs to Basidiomycotina, Laminomycetes, Agaricaceae, Agaricaceae, and Flammulina genus. It is famous for its smooth and tender cap, crisp handle, rich nutrition, and delicious taste. Fujian Province is the main planting area of ​​Flammulina velutipes in South China. With the expansion of the cultivation scale of Flammulina velutipes, the market has produced higher and higher standards for judging the cultivation, color and yield of Flammulina velutipes, such as the thickness and uniformity of the stipe, the gap of the cut surface, the brittleness of the stipe, and freshness. Persistence, cap size, mushroom body color and other evaluation criteria. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/6869C12Q1/04C12R1/645
CPCC12Q1/6869C12R2001/645C12N1/145C12Q2531/113C12Q2539/10
Inventor 江玉姬陈炳智刘芳岑光红蔡鹏赖丽红王瑞清李博王松谢宝贵
Owner FUJIAN AGRI & FORESTRY UNIV
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