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Sheep placenta extracting solution with anti-aging function as well as preparation method and application thereof

A sheep placenta and extract technology, which is applied in the direction of medical preparations containing active ingredients, skin care preparations, pharmaceutical formulas, etc., can solve the problem of difficulty in ensuring the type and content of natural active substances, insufficient extraction of biological active ingredients, and unfavorable Sheep placenta extract quality and other issues to achieve the effect of strengthening the activity of cortical cells, delaying aging, and stabilizing chemical properties

Active Publication Date: 2020-12-11
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing methods for extracting sheep placenta, most of them adopt enzymatic hydrolysis and high-temperature sterilization in the process. Enzymatic hydrolysis and high temperature will destroy the natural active substances in the placenta, making it difficult to guarantee the types and contents of natural active substances. It is beneficial to ensure the quality of sheep placenta extract, and the extraction efficiency is not high
Moreover, the anti-aging effect of sheep embryo / sheep placenta extract in the prior art is too general, and the bioactive components with anti-aging effect have not been fully extracted.

Method used

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  • Sheep placenta extracting solution with anti-aging function as well as preparation method and application thereof
  • Sheep placenta extracting solution with anti-aging function as well as preparation method and application thereof
  • Sheep placenta extracting solution with anti-aging function as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0048] A preferred embodiment of the present invention provides a method for preparing sheep placenta extract with anti-aging function, the specific steps are as follows:

[0049] 1. Wash and cut into pieces: Take the sheep placenta in the embryo stage to separate the cotyledon, discard the fetal membrane, wash the cotyledon 5 times with normal saline containing 100u / mL kanamycin until the blood is removed, and cut the cotyledon into about 1cm 3 Tissue blocks of that size are ready for use.

[0050] 2. Virus inactivation: soak in 0.1vt% peracetic acid solution for 10 minutes, and wash with purified water for 3 times to remove peracetic acid residue after treatment.

[0051] 3. Crushing and homogenizing: Add pre-cooled 1wt% taurine solution to the soaked cotyledon tissue block at a ratio of 1:3 in mass volume ratio for crushing and homogenizing at a speed of 15,000 rpm, homogenizing twice, 3 minutes each time.

[0052] 4. Extraction and centrifugation: place the homogeneous she...

Embodiment 2

[0059] A preferred embodiment of the present invention provides a method for preparing sheep placenta extract with anti-aging function, the specific steps are as follows:

[0060] 1. Wash and cut into pieces: take the sheep placenta at the embryonic stage to separate the cotyledons, discard the fetal membranes, wash the cotyledons three times with physiological saline containing 90u / mL kanamycin until the blood is removed, and cut the cotyledons into about 1cm 3 Tissue blocks of that size are ready for use.

[0061] 2. Virus inactivation: Soak in 0.08vt% peracetic acid solution for 10 minutes, and wash with purified water for 3 times to remove peracetic acid residue after treatment.

[0062] 3. Crushing and homogenizing: add pre-cooled 1wt% taurine solution to the soaked cotyledon tissue block at a mass volume ratio of 1:2.5 for crushing and homogenizing at a speed of 15,000 rpm, homogenizing twice, 3 minutes each time.

[0063] 4. Extraction and centrifugation: place the ho...

Embodiment 3

[0070] A preferred embodiment of the present invention provides a method for preparing sheep placenta extract with anti-aging function, the specific steps are as follows:

[0071] 1. Wash and cut into pieces: Take the sheep placenta in the embryo stage to separate the cotyledons, discard the fetal membranes, wash the cotyledons 4 times with normal saline containing 110u / mL kanamycin until the blood is removed, and cut the cotyledons into about 1cm 3 Tissue blocks of that size are ready for use.

[0072] 2. Virus inactivation: Soak in 0.12vt% peracetic acid solution for 10 minutes, and wash with purified water for 3 times to remove peracetic acid residue after treatment.

[0073] 3. Crushing and homogenizing: add pre-cooled 1wt% taurine solution to the soaked cotyledon tissue block at a mass volume ratio of 1:3.2 for crushing and homogenizing at a speed of 15000rpm, homogenizing twice, 3 minutes each time.

[0074] 4. Extraction and centrifugation: place the homogeneous sheep...

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Abstract

The invention discloses a sheep placenta extracting solution with an anti-aging function as well as a preparation method and application thereof. The preparation method comprises the following steps of S1, taking sheep placenta in an embryo period, separating cotyledons, discarding fetal membranes, cleaning the cotyledons with normal saline, and dicing the cotyledons; S2, soaking the cotyledon blocks in a peroxyacetic acid solution, and then cleaning the cotyledon blocks with purified water; S3, adding a taurine solution into the cotyledon blocks, and carrying out crushing and homogenizing toobtain slurry; S4, oscillating and centrifuging the slurry, and separately collecting supernatant and precipitate; S5, adding taurine into the precipitate, carrying out negative pressure cavitation extraction, performing centrifuging, and collecting the supernatant; S6, mixing the supernatant, adjusting the pH value, oscillating, centrifuging, and collecting the supernatant; S7, adjusting the pH value of the supernatant, carrying out complementing and inactivating, then adjusting the pH value, performing centrifuging, and collecting supernatant; and S8, carrying out ultrafiltration concentration on the supernatant, and performing filtering and degerming to obtain the product. The extract disclosed by the invention has the effects of moisturizing, resisting wrinkles, delaying senescence andenhancing the skin immune function when being applied to cosmetics, and has a wide market prospect.

Description

technical field [0001] The invention belongs to the technical field of extract preparation, in particular to a sheep placenta extract with anti-aging function and its preparation method and application. Background technique [0002] Aging is an inevitable stage in the life activity process of any organism. As people grow older, people are affected by various factors inside and outside the body, and the body will inevitably experience the aging process of the body's morphological structure and physiological functions. As the largest and most easily exposed organ of the body, the skin is responsible for many aspects of body protection, sensation, temperature regulation, secretion, excretion and immunity, and is also one of the most obvious organs in body aging. In recent years, the conclusion that ultraviolet rays cause skin photoaging has been widely accepted. Ultraviolet rays can be divided into short, medium and long-wave ultraviolet rays according to their wavelengths. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61Q19/08A61Q19/00A61P17/00A61P37/04
CPCA61K8/982A61Q19/08A61Q19/00A61Q19/004A61P17/00A61P37/04A61K2800/805
Inventor 张向飞胡忠国王翔王洁朱熠樊红柳肖静
Owner CHENGDU QINGKE BIOTECH
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