Sheep placenta extracting solution with anti-aging function as well as preparation method and application thereof
A sheep placenta and extract technology, which is applied in the direction of medical preparations containing active ingredients, skin care preparations, pharmaceutical formulas, etc., can solve the problem of difficulty in ensuring the type and content of natural active substances, insufficient extraction of biological active ingredients, and unfavorable Sheep placenta extract quality and other issues to achieve the effect of strengthening the activity of cortical cells, delaying aging, and stabilizing chemical properties
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Embodiment 1
[0048] A preferred embodiment of the present invention provides a method for preparing sheep placenta extract with anti-aging function, the specific steps are as follows:
[0049] 1. Wash and cut into pieces: Take the sheep placenta in the embryo stage to separate the cotyledon, discard the fetal membrane, wash the cotyledon 5 times with normal saline containing 100u / mL kanamycin until the blood is removed, and cut the cotyledon into about 1cm 3 Tissue blocks of that size are ready for use.
[0050] 2. Virus inactivation: soak in 0.1vt% peracetic acid solution for 10 minutes, and wash with purified water for 3 times to remove peracetic acid residue after treatment.
[0051] 3. Crushing and homogenizing: Add pre-cooled 1wt% taurine solution to the soaked cotyledon tissue block at a ratio of 1:3 in mass volume ratio for crushing and homogenizing at a speed of 15,000 rpm, homogenizing twice, 3 minutes each time.
[0052] 4. Extraction and centrifugation: place the homogeneous she...
Embodiment 2
[0059] A preferred embodiment of the present invention provides a method for preparing sheep placenta extract with anti-aging function, the specific steps are as follows:
[0060] 1. Wash and cut into pieces: take the sheep placenta at the embryonic stage to separate the cotyledons, discard the fetal membranes, wash the cotyledons three times with physiological saline containing 90u / mL kanamycin until the blood is removed, and cut the cotyledons into about 1cm 3 Tissue blocks of that size are ready for use.
[0061] 2. Virus inactivation: Soak in 0.08vt% peracetic acid solution for 10 minutes, and wash with purified water for 3 times to remove peracetic acid residue after treatment.
[0062] 3. Crushing and homogenizing: add pre-cooled 1wt% taurine solution to the soaked cotyledon tissue block at a mass volume ratio of 1:2.5 for crushing and homogenizing at a speed of 15,000 rpm, homogenizing twice, 3 minutes each time.
[0063] 4. Extraction and centrifugation: place the ho...
Embodiment 3
[0070] A preferred embodiment of the present invention provides a method for preparing sheep placenta extract with anti-aging function, the specific steps are as follows:
[0071] 1. Wash and cut into pieces: Take the sheep placenta in the embryo stage to separate the cotyledons, discard the fetal membranes, wash the cotyledons 4 times with normal saline containing 110u / mL kanamycin until the blood is removed, and cut the cotyledons into about 1cm 3 Tissue blocks of that size are ready for use.
[0072] 2. Virus inactivation: Soak in 0.12vt% peracetic acid solution for 10 minutes, and wash with purified water for 3 times to remove peracetic acid residue after treatment.
[0073] 3. Crushing and homogenizing: add pre-cooled 1wt% taurine solution to the soaked cotyledon tissue block at a mass volume ratio of 1:3.2 for crushing and homogenizing at a speed of 15000rpm, homogenizing twice, 3 minutes each time.
[0074] 4. Extraction and centrifugation: place the homogeneous sheep...
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