A strain of Bacillus velesi and its sieving culture method and application
A technology of Bacillus velesi and Bacillus, applied in the field of environmental microorganisms, can solve problems such as single fixation of degrading bacteria species, and achieve the effects of remarkable treatment effect, good application prospect and strong resistance to stress.
Active Publication Date: 2022-07-26
BC P INC CHINA NAT PETROLEUM CORP +1
View PDF6 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
The degrading bacteria provided by these patents are all compound strains, and the types of degrading bacteria involved are relatively single and fixed.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
example 1
[0037](1) Isolation, purification and preservation of Bacillus velezensis B3-4 (referred to as "strain B3-4")
[0038] Collected human fecal samples, cryopreserved and brought back to the laboratory. Take 5 g of fecal samples, spread them on beef extract peptone medium by gradient dilution method, and cultivate at a constant temperature of 30°C. After the colonies were grown, the colonies with different morphology were selected from the plate for streaking culture on the beef extract peptone plate. After culturing for 16 h, pick a ring for repeated streaking and cultivation, combined with microscopic observation, until purification. The purified strains were stored in the test tube slant medium of beef extract peptone medium.
[0039] As shown in Figure 1, the bacterial strain B3-4 obtained by the separation and purification of this example was cultured on the beef extract peptone medium. After culturing for 48 hours, the colonies were round, white, 0.5-1 mm in diameter, wit...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Login to View More
Abstract
The present invention provides a strain of Bacillus velesi and its sieving and culturing method and application. The classification name of the Bacillus velezensis is Bacillus velezensis B3-4, and the strain has been deposited in the China Center for Type Culture Collection on May 29, 2020, and its deposit number is CCTCC NO: M 2020160. The sieving culture method includes the following steps: collecting samples and storing them at low temperature; applying the dilution of the samples on the beef extract peptone medium by gradient dilution method, and culturing at a constant temperature of 15-50° C.; after the culture medium grows colonies, Colonies with different shapes were selected for streak culture; then, a ring of colonies was picked for repeated streak culture, and combined with microscopic observation, until purified strains were obtained. The Bacillus velesi can effectively degrade and remove organic matter and COD in human feces and domestic sewage.
Description
technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a strain of Bacillus velesi and a screening and culturing method and application thereof. Background technique [0002] As early as the early 2000s, the state put forward the strategic goal of toilet renovation, taking toilet renovation as an important project to transform the environment and create a healthy human settlement. [0003] As a product of the "toilet revolution", environmentally friendly toilets have undergone continuous development. At present, there are two types of environmentally friendly toilets that are widely used: water flushing and water-free flushing. The flush-free biodegradable flush-free toilets with functions such as flushing and fecal recycling have received a lot of attention. However, the technical emphasis is on the design of the equipment structure, and the microbial strain resources in the biological treatment are less involv...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C02F11/02C02F101/30C12R1/07
CPCC12N1/02C12N1/20C02F3/34C02F11/02C02F2101/30C12R2001/07C12N1/205
Inventor 刘汉军陈雷李辉张薇陈立荣蒋学彬张敏李盛林闫瑞景周鋆文炜涛孟召伟吴思斯毛红敏徐梓培孙玉
Owner BC P INC CHINA NAT PETROLEUM CORP