Cold storage premixed kit and use method

Abstract

The invention relates to a cold storage premixed reagent kit and a use method. Related reagents are premixed according to an experimental process to form a premixed reagent, the premixed reagent is loaded into the kit, experimental operation steps can be effectively simplified, and besides, long-term effective storage of the reagent under a cold storage condition is realized by performing targetedadjustment on premixed reagent components. The tedious operation of repeated freezing and thawing when the kit is used in an experiment is avoided, so that the experiment process is remarkably simplified.

Description

technical field [0001] The invention relates to the technical field of gene detection library preparation, in particular to a refrigerated storage premix kit and a use method. Background technique [0002] High-throughput sequencing technology (High-throughput Sequencing) is a parallel sequence determination of hundreds of thousands to millions of DNA molecules, also known as next-generation sequencing technology (Next-Generation Sequencing), second-generation sequencing technology, which makes it possible to In-depth, detailed, and comprehensive analysis of the genome and transcriptome of a species is also called Deep Sequencing. [0003] The existing kits for high-throughput sequencing all focus on the basic principles of the experiment. Except for the magnetic beads, the kits are all stored at -20 degrees. When using them, the kit components need to be thawed at room temperature in advance, and then the components are configured before subsequent follow-up. The operation...

AI Technical Summary

Problems solved by technology

[0003] The existing kits for high-throughput sequencing all focus on the basic principles of the experiment. Except for the magnetic beads, the kits are all stored at -20 degrees. When using them, the kit components need to be thawed at room temperature in advance, and then the components are configured before subsequent follow-up. The operation, especially in some cases, requires repeated freezing and thawing and proportioning. There are many kinds of reagents stored independently in the kit. The use process involves melting and mixing and centrifugation of different reagents. The operation is complicated and the requirements for the operating environment are also high.

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