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Immobilized microorganism soil remediation agent and soil remediation method thereof

A soil remediation agent and soil remediation technology, applied in the field of environmental protection, can solve the problems of poor removal effect of polycyclic aromatic hydrocarbons, poor water solubility of polycyclic aromatic hydrocarbons, etc., and achieve the effects of facilitating colonization of strains, promoting absorption effect, and increasing concentration.

Inactive Publication Date: 2020-12-25
森科环保新材料(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The soil remediation prepared by the above patents and the prior art has poor removal effect on polycyclic aromatic hydrocarbon organic matter, and due to the poor water solubility of polycyclic aromatic hydrocarbons, it has an inhibitory effect on microbial growth and its special and stable ring structure, making it difficult to be removed Biopharmaceuticals in general break down so they tend to accumulate in the environment

Method used

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  • Immobilized microorganism soil remediation agent and soil remediation method thereof
  • Immobilized microorganism soil remediation agent and soil remediation method thereof
  • Immobilized microorganism soil remediation agent and soil remediation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] An immobilized microbial soil remediation agent and a soil remediation method thereof, the specific preparation scheme of which is as follows:

[0039] Take 100g of microbial culture solution, divide it into 4g on average, inoculate nitrifying bacteria, denitrifying bacteria, phosphorus accumulating bacteria and Burkholderia respectively, and then culture at 27°C for 15 hours. When the number of viable bacteria reaches 1.0×10 9 When the CFU / ml is above, mix the culture solution of the four strains evenly, add 80g of normal saline, stir evenly, add 10g of biomass activated carbon, stir and absorb for 120min, add 5g of repair synergist after completion, stir for 10min and then stir rapidly Slowly add 100g of calcium chloride solution with a mass percentage of 0.5% into the reactor, stir to obtain gel particles, control the temperature at 30°C, cross-link at room temperature for 24 hours, and dry to obtain the immobilized microbial soil remediation agent.

[0040] The micr...

Embodiment 2

[0053] An immobilized microbial soil remediation agent and a soil remediation method thereof, the specific preparation scheme of which is as follows:

[0054] Take 130g of microbial culture solution, divide it into 4g on average, inoculate nitrifying bacteria, denitrifying bacteria, phosphorus accumulating bacteria and Burkholderia respectively, and then culture at 29°C for 18 hours. When the number of viable bacteria reaches 1.0×10 9 When the CFU / ml is above, mix the culture solution of the four strains evenly, add 100g of normal saline, stir evenly, add 13g of activated carbon, stir and absorb for 150min, add 8g of repair synergist after completion, stir for 20min and then stir rapidly Slowly add 150 g of calcium chloride solution with a mass percent content of 1.5% into the reactor, stir to obtain gel particles, control the temperature at 35°C, cross-link at room temperature for 36 hours, and dry to obtain the immobilized microbial soil remediation agent.

[0055] The micro...

Embodiment 3

[0068] An immobilized microbial soil remediation agent and a soil remediation method thereof, the specific preparation scheme of which is as follows:

[0069] Take 150g of microbial culture solution, divide it into 4g on average, inoculate nitrifying bacteria, denitrifying bacteria, phosphorus accumulating bacteria and Burkholderia respectively, and then culture at 31°C for 20 hours. When the number of viable bacteria reaches 1.0×10 9 When the CFU / ml is above, mix the culture solution of the four strains evenly, add 120g of normal saline, stir evenly, add 15g of biomass activated carbon, stir and absorb for 180min, add 12g of repair synergist after completion, stir for 30min and then stir rapidly Slowly add 200g of calcium chloride solution with a mass percentage content of 2% into the reactor, stir to obtain gel particles, control the temperature at 40°C, cross-link at room temperature for 48 hours, and dry to obtain the immobilized microbial soil remediation agent.

[0070] ...

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Abstract

The invention relates to the field of environmental protection, in particular to an immobilized microbial soil remediation agent and a soil remediation method thereof. The immobilized microorganism soil remediation agent is prepared by taking charcoal as a carrier, and the microorganism remediation agent has a good remediation effect on soil polluted by polycyclic aromatic hydrocarbon. According to the immobilized microorganism soil remediation agent, microorganisms are immobilized in a limited space area, so that the concentration of microorganism cells is increased, high biological activityof the microorganism cells is kept, and the concentration of useful substances such as amino acid, organic acid and antibiotics secreted by the microorganisms and the biological catalysis effect are improved; bacterial strain colonization is facilitated, and degradation of pollutants is promoted; according to the method, a small amount of protein source is loaded in activated carbon to facilitatesurvival and proliferation of microorganisms; solid waste resources are effectively applied to contaminated soil remediation, and the method is a win-win environment-friendly means; and the soil remediation method has the advantages of being convenient to operate, low in cost, environmentally friendly and good in effect.

Description

technical field [0001] The invention relates to the field of environmental protection, in particular to an immobilized microbial soil remediation agent and a soil remediation method thereof. Background technique [0002] Soil pollutants can be directly ingested by the human body, and may even accumulate in the body, affecting the biochemical and physiological reactions of the human body, thereby affecting metabolism, development and reproductive functions, and may also affect the level of human intellectual development and damage the nervous system and endocrine system. With the in-depth understanding of the migration, transformation, pollution and toxicity of various pollutants in the environment, people realize that the research on the pollution of polycyclic aromatic hydrocarbons in the environment and the development of corresponding bioremediation technologies are urgent. [0003] CN105349153B discloses a medicament for soil remediation and its preparation method and ap...

Claims

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Application Information

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IPC IPC(8): C09K17/40B09C1/00B09C1/08B09C1/10C08B30/12C08F251/00C01B32/342C01B32/324C09K109/00
CPCB09C1/00B09C1/08B09C1/10C08B30/12C08F251/00C09K17/40C09K2109/00C01B32/324C01B32/342C08F230/085
Inventor 不公告发明人
Owner 森科环保新材料(广州)有限公司
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