Construction method and application of specific electrochemical sensor for tumor marker detection

A technology of tumor markers and construction methods, which is applied in the field of construction of specific electrochemical sensors, and can solve problems such as Ru nanomaterials changing electrochemical signals

Active Publication Date: 2020-12-25
UNITED POWER PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on whether Ru nanomaterials can change their electrochemical signals with the help of light energy.

Method used

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  • Construction method and application of specific electrochemical sensor for tumor marker detection
  • Construction method and application of specific electrochemical sensor for tumor marker detection
  • Construction method and application of specific electrochemical sensor for tumor marker detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for constructing a ratiometric electrochemical sensor for tumor marker detection, specifically comprising the following steps:

[0027] (1) Preparation of Ru / glassy carbon electrode:

[0028] Use alumina polishing powder with a particle size of 0.02 μm, mix it with ultrapure water, polish and polish the glassy carbon electrode with a diameter of 2 mm, and then use ultrapure water and ethanol to ultrasonically wash it, and dry it with ultrapure nitrogen. 2 μL of RuNPs solution was evenly coated on the surface of the electrode, and left at room temperature for 1 h to obtain a Ru / glassy carbon electrode;

[0029] (2) Electrochemical activity detection of Ru / glassy carbon electrode under dark and light conditions:

[0030] A three-electrode system was used, and the Ru / glassy carbon electrode obtained in the previous step was placed in PBS buffer solution (volume 2 mL, pH 7.0, concentration 0.005 mol / L). Under the conditions of darkness and light respectively (the...

Embodiment 2

[0037] A method for constructing a ratiometric electrochemical sensor for tumor marker detection, specifically comprising the following steps:

[0038] (1) Preparation of Ru / glassy carbon electrode:

[0039] Use alumina polishing powder with a particle size of 0.05 μm, mix it with ultrapure water, polish and polish the glassy carbon electrode with a diameter of 4 mm, and then use ultrapure water and ethanol to ultrasonically wash it, and dry it with ultrapure nitrogen. 5 μL of RuNPs solution was evenly coated on the surface of the electrode, and left at room temperature for 1.5h to obtain a Ru / glassy carbon electrode;

[0040] (2) Electrochemical activity detection of Ru / glassy carbon electrode under dark and light conditions:

[0041] A three-electrode system was used, and the Ru / glassy carbon electrode obtained in the previous step was placed in PBS buffer solution (volume 3 mL, pH 7.4, concentration 0.01 mol / L). Under dark and light conditions (the light conditions are: u...

Embodiment 3

[0048] A method for constructing a ratiometric electrochemical sensor for tumor marker detection, specifically comprising the following steps:

[0049] (1) Preparation of Ru / glassy carbon electrode:

[0050] Use alumina polishing powder with a particle size of 0.08 μm, mix it with ultrapure water, polish and polish the glassy carbon electrode with a diameter of 6 mm, and then use ultrapure water and ethanol to ultrasonically wash it, and dry it with ultrapure nitrogen. 8 μL of RuNPs solution was evenly coated on the surface of the electrode, and left at room temperature for 2 hours to obtain a Ru / glassy carbon electrode;

[0051] (2) Electrochemical activity detection of Ru / glassy carbon electrode under dark and light conditions:

[0052] Using a three-electrode system, place the Ru / glassy carbon electrode obtained in the previous step in PBS buffer solution (volume 4 mL, pH 7.5, concentration 0.015 mol / L). Under dark and light conditions (the light conditions are: use yello...

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Abstract

The invention provides a preparation method of Ru nanoparticles and research on electrochemical activity of the Ru nanoparticles, and belongs to the field of electrochemical application, wherein a sensor for detecting tumor markers is constructed by utilizing electrochemical signals of the Ru nanoparticles. The invention mainly discloses that a Ru nano material has a unique electrochemical oxidation peak; and the change of the electrochemical oxidation peak of the Ru nano material under an illumination condition is explored, and the electrochemical sensor for detecting the tumor markers is designed by utilizing the characteristic. The method is simple and easy to implement and has high detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of electrochemical analysis, and in particular relates to a construction method and application of a specific electrochemical sensor for tumor marker detection. Background technique [0002] Electrochemical redox active probes are the basis for building stable electrochemical biosensors, and are the key to the development and clinical application of rapid detection electrodes. Compared with electroactive organic molecules, electroactive nanomaterials have unique electronic properties and fast electron transfer efficiency without additional carrier loading, and electrochemical redox signals with tunable intensity and optional peak positions can be obtained by adjusting size, composition and structure. , to meet the specific needs of biological applications. However, the research on electroactive nanomaterials themselves as signaling labels is still in its infancy, with only a few reports. Moreover, the rese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 赵媛刘瀚
Owner UNITED POWER PHARMA TECH CO LTD
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