Plant fermentation product and preparation method and application thereof in stomach health care
A plant fermentation and plant extract technology, applied in the field of plant fermentation and its preparation, and stomach health care, can solve problems such as affecting health, unhealthy eating habits, affecting the quality of drinking life, etc., and achieve the effect of reducing inflammation and improving activity.
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[0034] The invention provides a plant fermented product and its preparation method and its use for stomach protection and health care. The plant fermented product of the present invention is prepared by mixing raspberry, yam, hawthorn and solvent in a ratio of 1-3:1-5:1:20- The ratio of 40 (w / w) is obtained by extracting at a specific time and temperature, first fermented with yeast and Lactobacillus plantarum, and then fermented with Acetobacter, wherein the yeast is a strain of BCRC20271, The Lactobacillus plantarum is a BCRC910805 strain, and the Acetobacter is a BCRC11688 strain. The plant fermented product of the present invention can effectively inhibit the growth of gastric Helicobacter pylori in vivo; reduce the expression levels of IL-1β gene, IL-23 gene, IL-12A gene, IL-3 gene, and TNF-α gene in gastric cells; And it can increase the expression of TGFβ gene.
[0035] At the same time, the composition for protecting the stomach and health care of the present inventio...
Embodiment 1
[0037] Embodiment 1 The preparation method of plant fermented product of the present invention
[0038] In one embodiment of the present invention, after mixing the radish seed, the tuber of yam, and the fruit of hawthorn in a weight percentage of 1-3:1-5:1-3, the mixture is mixed with water, alcohol, or a mixture of alcohol and water The extraction solvent is uniformly mixed at 3-11:20-40 weight percent, wherein the extraction solvent is preferably water, and after simultaneous sterilization and extraction at 50-100 ° C for 0.5-3 hours, the plant water extract is cooled to room temperature For the subsequent two-stage fermentation, the following three types of bacteria are used for the two-stage fermentation in sequence: yeast and Lactobacillus plantarum are co-cultured for fermentation, and then Acetobacter is used for fermentation. This fermentation sequence cannot be reversed, and its fermentation The time ratio is 1-5:3-15. In a preferred embodiment of the present invent...
Embodiment 2
[0039] Embodiment 2 Plant fermented product of the present invention inhibits the effect of gastric Helicobacter pylori growth
[0040] One embodiment of the present invention is to carry out the two-stage fermentation step of the present invention to improve the efficacy test of the plant fermented product inhibiting the growth of gastric Helicobacter pylori, and utilize the inhibition zone method to test its antibacterial efficacy, wherein the inhibition zone method is also called the diffusion method, It is a method of using the substance to be tested to diffuse in agarose gum to inhibit the growth of bacteria around it to form a transparent circle, that is, the zone of inhibition, and to determine the antibacterial effect of the substance to be tested according to the size of the zone of inhibition. It is often used in Isolation and screening of antibiotic-producing bacteria.
[0041] First, 1% gastric Helicobacter pylori (purchased from the Biological Resource Conservatio...
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