Cell negative pressure culture device
A culture device and cell culture technology, applied in the field of medical devices, can solve problems such as inability to inject culture fluid, and achieve the effect of ensuring accuracy and avoiding contamination
Pending Publication Date: 2021-01-12
杭州归领医疗器械有限公司
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Problems solved by technology
[0006] Aiming at the deficiencies of the prior art, the present invention provides a cell negative pressure culture device, which solves the problem that the cell negative pressure culture device cannot inject culture solution in real time under the aseptic condition of keeping the negative pressure seal
Method used
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[0034] like figure 1 , figure 2 As shown, the present invention provides a cell negative pressure culture device, said cell negative pressure culture device comprises: cell culture porous plate 10, cylindrical culture medium plate 20, cell culture vessel 30, silica gel sealing cover 40, the first needle 41 and the second needle 42;
[0035] The cell culture porous plate 10 is provided with a number of culture tanks 11; the bottom surface of the cell culture vessel 30 is made of a cultured polycarbonate porous membrane; the cell culture vessel 30 is provided with a foam 31;
[0036] The culture medium plate 20 is inserted into the culture tank 11, and the cell culture vessel 30 is inserted into the culture medium plate 20; the outer cylinder wall of the culture medium plate 20 is in sealing connection with the inner wall of the culture tank 11, and the outer wall of the cell culture vessel 30 is connected with the culture tank 11. The inner cylinder wall of the culture plate...
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The invention provides a cell negative pressure culture device, and relates to the technical field of medical instruments. The cell negative pressure culture device comprises a cell culture porous plate, a cylindrical culture substrate, a cell culture vessel, a silica gel sealing cover, a first needle head and a second needle head, wherein the cell culture porous plate is provided with a pluralityof culture grooves; the bottom surface of the cell culture vessel is made of a porous membrane; and foam is arranged in the cell culture vessel. According to the invention, the culture substrate is in sealed insertion connection with the culture grooves, the cell culture vessel is in sealed insertion connection with the culture substrate, and the silica gel sealing cover seals the top opening ofthe cell culture vessel, so that a sterile sealed environment is provided for cell culture; a negative pressure pump provides negative pressure for the culture environment of cells through the first needle head; a culture solution is injected into the groove bottoms of the culture grooves through the second needle head, so that the culture solution is injected in real time under the sterile condition of keeping negative pressure sealing; and the accuracy of observing cell culture is guaranteed while the cells are effectively prevented from being polluted.
Description
technical field [0001] The invention relates to the technical field of medical devices, in particular to a cell negative pressure culture device. Background technique [0002] In medical research, it is sometimes necessary to observe the growth of cells in a negative pressure environment. [0003] At present, in order to observe the growth of cells in a negative pressure environment, cells are usually cultured on cell culture multi-well plates and cell culture vessels, and a quantitative amount of culture solution is injected at one time before the negative pressure is sealed. [0004] During the cultivation process, if it is necessary to continue to inject the culture medium, the negative pressure sealing state of the cell culture device must be released. In the process of injecting the culture solution, the sterile sealed environment and negative pressure state of the cell culture device are destroyed, which is easy to contaminate the cells and affect the accuracy of obse...
Claims
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Login to View More IPC IPC(8): C12M3/06C12M3/00
CPCC12M23/02C12M23/12C12M23/20C12M23/38C12M29/04C12M29/14C12M37/04
Inventor 洪章鹏时晨赵中
Owner 杭州归领医疗器械有限公司