Application of chicken molecular marker combination as detection site for authenticating intermuscular fat width of chicken

A molecular marker, intermuscular fat technology, applied in the determination/inspection of microorganisms, recombinant DNA technology, DNA/RNA fragments, etc., can solve the problems of affecting the fitness effect, changing the nutritional ratio of chicken, etc., to achieve segmented consumer market, The effect of boosting sales and accelerating genetic progress

Active Publication Date: 2021-01-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the increase of intermuscular fat will increase the fat content of chic

Method used

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  • Application of chicken molecular marker combination as detection site for authenticating intermuscular fat width of chicken
  • Application of chicken molecular marker combination as detection site for authenticating intermuscular fat width of chicken
  • Application of chicken molecular marker combination as detection site for authenticating intermuscular fat width of chicken

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Primer Design

[0043] 1. Experimental method

[0044] Find the sequence of the 3' non-coding region of the ALDH1A3 gene from https: / / www.ncbi.nlm.nih.gov / , and design a primer pair. The primer pair information is shown in Table 1 (primer sequence 5'→3'). The sequence of the primer pair was sent to Guangzhou Sangon Biotech Co., Ltd. for synthesis, and the primer pair was used to perform PCR on blood DNA to test the specificity of the primers.

[0045] Table 1 Primer information for SNP screening of ALDH1A3

[0046]

[0047] 2. Experimental results

[0048] The results are attached figure 1 shown. Use this primer pair to carry out PCR on blood DNA, as attached figure 1 As shown, the primers are specific.

Embodiment 2

[0049] Example 2 DNA pool sequencing

[0050] 1. Experimental method

[0051] The sample chickens were selected from 600 120-day-old Nanhai Jute Chicken No. 1 pure line of Jiangfeng Chicken Farm. All chickens were hatched in the same batch, raised in a flat way, and fed with corn-soybean meal that meets international formula standards feed. Before slaughter, blood was collected from the subwing vein with a 2 mL syringe, and the collected blood was stored in a centrifuge tube containing 20% ​​EDTA for blood DNA extraction.

[0052] Genomic DNA of all individuals based on Plant Mini Kit (Qiagen, Hilden, CA; Cat#69104) was used in the kit instructions to extract blood DNA, measure the quality and concentration, dilute to 50 ng / μL, and store at 4°C for later use.

[0053] 30 DNA samples were randomly selected from the total extracted DNA samples to construct a mixed pool, and every three samples (about 0.33 μL of each sample) were mixed into a mixed sample, a total of 10 sampl...

Embodiment 3

[0056] Example 3 Determination of haplotypes of ALDH1A3 mutation sites g.355T>C and g.405A>G

[0057] 1. Experimental method

[0058] Before slaughtering, all chicken samples were tested for indicators, in accordance with the Agricultural Industry Standard of the People's Republic of China "Poultry Production Performance Terminology and Measuring and Statistical Methods" (NT / T823-2004). Among them, the subcutaneous fat thickness and intermuscular fat width were measured with a vernier caliper, and the shin circumference and shin length were measured with a soft ruler. After the chickens were bled, scalded and plucked in a unified line in the slaughterhouse, they were gently wiped dry with paper towels, and the cloaca, shin (cuticle removal), Determination of yellowness values ​​of upper back, lower back, chest, abdomen and abdominal fat.

[0059] A database was established with Excel, and individuals with incomplete trait data records and outliers were deleted during the ini...

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Abstract

The invention discloses application of a chicken molecular marker combination as a detection site for authenticating the intermuscular fat width of a chicken. The molecular marker comprises a molecular marker 1 and a molecular marker 2 which are independently a chicken ALDH1A3 gene 3' noncoding region sequence g.355 T>C molecular marker and a chicken ALDH1A3 gene 3' noncoding region sequence g.405A>G molecular marker. When the molecular marker combination is used for carrying out marker assisted selection, a chicken flock with the large intermuscular fat width or the small intermuscular fat width can be directionally bred, chicken breeds with different intermuscular fat widths can be cultured by aiming at different breeding targets, and chicken breeding progress is quickened. Through the molecular marker combination and a detection primer, an efficient and accurate molecular marker assisted breeding technology can be established, then, the efficient and accurate molecular marker assisted breeding technology is applied to genetic improvement of the intermuscular fat width character of a breeding chicken so as to culture different chicken strains with intermuscular fat width difference, the chicken breeds with different intermuscular fat widths are sold by aiming at different consumer groups, a consumer market is further subdivided, the sales volume of the chickens is accelerated, the sales of enterprises can be improved, and core competitiveness is increased.

Description

technical field [0001] The invention relates to the technical field of molecular breeding, and more particularly relates to the application of a combination of chicken molecular markers as a detection site for identifying chicken intermuscular fat width. Background technique [0002] Chicken is a kind of traditional edible poultry. my country is a big chicken consumption country. In 2018, the total consumption of chicken in my country was 11.53 million tons, accounting for 11% of the total global consumption of chicken. In addition, my country is also the third largest chicken producer in the world, second only to the United States and Brazil. [0003] People's food demand structure and nutritional demand structure will change with the improvement of economic development level and people's quality of life. The main performance is that with the improvement of living standards, people's demand for grains with more carbohydrates will gradually The demand for meat with more pro...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 罗文吴静文张细权聂庆华
Owner SOUTH CHINA AGRI UNIV
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