Composite drug-loading nanoscale ultrasonic multifunctional microbubble based on three lesions and preparation method thereof

A drug-loaded nanometer and multi-functional technology, applied in the field of biomedicine, to achieve good biological safety, prolong circulation time, and enhance the effect of abdominal aorta ultrasound imaging

Pending Publication Date: 2021-01-22
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently, there is no CNTs and Fe 3 o 4 Research on Nanoparticle Junction Applied to Ultrasound Contrast Agents

Method used

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  • Composite drug-loading nanoscale ultrasonic multifunctional microbubble based on three lesions and preparation method thereof
  • Composite drug-loading nanoscale ultrasonic multifunctional microbubble based on three lesions and preparation method thereof
  • Composite drug-loading nanoscale ultrasonic multifunctional microbubble based on three lesions and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0049] Specific embodiment one: a compound drug-loaded nano-scale ultrasonic multifunctional microbubble based on three types of lesions, characterized in that the microbubbles include: microbubble membrane wall, Fe 3 o 4 -CNTs-ASA, Fe 3 o 4 -CNTs-GM or Fe 3 o 4 -CNTs-DOX magnetic targeting drug-loaded complex, and the inert gas wrapped inside the microbubble membrane wall;

[0050] The targeting factor Fe 3 o 4 On deposited CNTs, Fe 3 o 4 -CNTs magnetic targeting complex loads drugs acetylsalicylic acid ASA, gentamicin GM or doxorubicin DOX through physical adsorption;

[0051] Part of Fe 3 o 4 -CNTs magnetic targeting complex bound to the outer wall of microvesicles, a part of Fe 3 o 4 -CNTs magnetic targeting complexes will also be encapsulated inside the microbubbles.

[0052] The inert gas is nitrogen.

[0053] Embodiment 2: The preparation method of the composite drug-loaded nano-scale ultrasonic multifunctional microbubble based on the three kinds of lesio...

Embodiment approach 3

[0072] Step 1 and step 2 are the same as the specific embodiment 2;

[0073] Composite Fe in step 3 3 o 4 -CNTs-ASA, Fe 3 o 4 -CNTs-GM or Fe 3 o 4 - Preparation method of CNTs-DOX microbubbles:

[0074] Weigh 3.5g of polyvinyl alcohol (PVA) and soak in 100mL of double distilled water for 3 hours, then stir in a water bath at 90°C until completely dissolved, and keep stirring for later use to obtain a PVA solution;

[0075] Add 0.6g of polylactic acid (PLA) into 12mL of dichloromethane (DCM), soak for 5h until completely dissolved to obtain an organic phase;

[0076] Take Fe 3 o 4 -CNTs-ASA, Fe 3 o 4 -CNTs-GM or Fe 3 o 4 - Add 0.1g of CNTs-DOX complex to 0.2mL ethanol aqueous solution as the inner water phase;

[0077] Add the inner water phase to the organic phase, perform ultrasonic emulsification at a certain switching frequency at a frequency of 25kHz, 90-150W, and continuously inject inert gas into the reaction system during the reaction process to obtain W / O ...

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Abstract

The invention discloses a composite drug-loading nanoscale ultrasonic multifunctional microbubble based on three lesions and a preparation method thereof, and belongs to the field of biomedical treatment. The microbubble comprises a microbubble membrane wall, Fe3O4-CNTs-ASA, Fe3O 4-CNTs-GM or Fe3O4-CNTs-DOX magnetic targeting drug-loading compound and inert gas wrapped in the microbubble membranewall. The prepared PLA microbubble of the composite Fe3O4-GO-ASA can effectively prolong the coagulation time, prevent the formation of thrombus and achieve the auxiliary treatment effect of anticoagulation. The prepared Span-PEG microbubble of the composite Fe3O4-CNTs-GM has a good inhibitory effect on escherichia coli and staphylococcus aureus, and the inhibitory effect on the escherichia coli is higher than that of the staphylococcus aureus. The prepared Span-PEG microbubble of the composite Fe3O4-CNTs-DOX has a proliferation inhibition effect on liver cancer SMMC-7721 cells and mouse normal fibroblast 3T3 cells, and compared with free DOX, the Span-PEG microbubble of the composite Fe3O4-CNTs-DOX can reduce the direct toxic effect of adriamycin on normal cells.

Description

technical field [0001] The invention belongs to the field of biomedicine; in particular, it relates to a composite drug-loaded nano-scale ultrasonic multifunctional microbubble based on three types of lesions and a preparation method thereof. Background technique [0002] Thrombosis is a common disease of the cardiovascular system, which is seriously harmful. Ultrasound has become an important detection method. Angiographic ultrasonography can improve the resolution of the intima of the vessel. The use of ultrasound contrast agent can more accurately and clearly judge the situation of intravascular obstruction, so that the measurement Increased accuracy. The harm of malignant tumors to human health is second only to cardiovascular diseases. Early diagnosis and treatment are important measures to improve the survival rate of cancer patients. At present, the drugs for tumor treatment mainly include paclitaxel and doxorubicin. Although they have a good tumor inhibitory effect,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/113A61K47/22A61K47/10A61K31/60A61K31/7036A61K31/704A61K49/22A61P35/00A61P7/02A61P29/00
CPCA61K9/113A61K47/22A61K47/10A61K31/60A61K31/7036A61K31/704A61K49/223A61P35/00A61P7/02A61P29/00Y02A50/30
Inventor 张杰李柏凝胡明李进京常存一吴义班宋立梅
Owner JIAMUSI UNIVERSITY
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