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A chemical mutagenesis method for inducing and increasing the mutation range of Banana

A technology of mutation range and chemical mutagenesis, which is applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of different tolerance, small variation range, and small development space, so as to increase mutation frequency, variation The effect of enriching types and expanding the range of variation

Active Publication Date: 2021-12-21
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the development space of new banana varieties cultivated by hybridization is small, the stress resistance of the varieties is not comprehensive, and the variation range is small, it is difficult to produce new gene mutants through natural mutation, and the current resistance to mutagens of bananas is limited. Different, although a single mutagenesis method has a high mutation frequency in a certain aspect, the mutation rate of the overall trait is still low, resulting in fewer mutation types and low efficiency of mutation breeding

Method used

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  • A chemical mutagenesis method for inducing and increasing the mutation range of Banana
  • A chemical mutagenesis method for inducing and increasing the mutation range of Banana

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Experimental program
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Effect test

Embodiment 1

[0022] A method for inducing mutagenesis increasing chemical mutant Fenjiao range: comprising the steps of:

[0023] Sl, formulated growth medium: MS medium, 6- benzylaminopurine (BAP) 1.0mg / L, adenine 0.2mg / L, agarose 2.0g / L, sucrose 20g / L, sucrose, pH 4.0;

[0024] Mutagenized S2, banana: using a scalpel cut at a single independent shoots, into the flask containing lithium chloride mutagenic agent, in a rotary shaker at 138rpm, 20 ℃ shaking culture after soaking 4h, then placed in the incubator light, illumination intensity is set 1000lx, then changing temperature, the initial process temperature is 15 ℃ 1h, adjust the temperature 22 ℃, IH processing; the mutagen is a lithium chloride solution of lithium chloride sterile water plus Tween-80 and banana peel polysaccharide solution, sterilized by filtration, the lithium chloride solution, sterilized water, the volume ratio of Tween-80 and banana peel polysaccharide solution is 1: 10: 0.3: 1 ;

[0025] S3, washing treatment...

Embodiment 2

[0028] A method for inducing mutagenesis increasing chemical mutant Fenjiao range: comprising the steps of:

[0029] Sl, formulated growth medium: MS medium, 6- benzylaminopurine (BAP) 5.0mg / L, adenine 1.5mg / L, agarose 4.0g / L, sucrose 40g / L, sucrose, pH 6.0;

[0030] Mutagenized S2, banana: using a scalpel cut at a single independent shoots, into the flask containing lithium chloride mutagenic agent, in a rotary shaker at 200rpm, 30 ℃ soaking shaking cultured 24h, then placed in the incubator light, illumination intensity is set 3000Lx, then changing temperature, the initial process temperature is 20 ℃ 3h, adjust the temperature 30 ℃, 3H process; the mutagen is a lithium chloride solution of lithium chloride sterile water plus Tween-80 and banana peel polysaccharide solution, sterilized by filtration, the lithium chloride solution, sterilized water, the volume ratio of Tween-80 and banana peel polysaccharide solution is 1: 20: 2: 5 ;

[0031] S3, washing treatment: After t...

Embodiment 3

[0034] A method for inducing mutagenesis increasing chemical mutant Fenjiao range: comprising the steps of:

[0035] Sl, formulated growth medium: MS medium, 6- benzylaminopurine (BAP) 3.0mg / L, adenine 1.0mg / L, agarose 3.0g / L, sucrose 30g / L;

[0036] Mutagenized S2, banana: using a scalpel cut at a single independent shoots, into the flask containing lithium chloride mutagenic agent, in a rotary shaker at 150rpm, 27 ℃ 14H cultured shaking immersion, then placed in the incubator light, illumination intensity is set 2000lx, then changing temperature, the initial process temperature is 18 ℃ 2h, adjust the temperature 26 ℃, 2H process; the mutagen is a lithium chloride solution of lithium chloride sterile water plus Tween-80 and banana peel polysaccharide solution, sterilized by filtration, the lithium chloride solution, sterilized water, the volume ratio of Tween-80 and banana peel polysaccharide solution is 1: 15: 1.2: 3 ;

[0037] S3, washing treatment: After the above proc...

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Abstract

The invention provides a chemical mutagenesis method for inducing and increasing the mutation range of bananas, comprising the following steps: preparation of proliferation medium, mutagenesis treatment of bananas, washing treatment, proliferation and cultivation, lithium chloride solution used in the invention to inhibit seedlings There are obvious differences in growth and growth, and individual plants with special traits appear in the form; through mutagen treatment, the mutation frequency is increased and the variation range is expanded. The use of chemical mutagen treatment can increase the mutation frequency up to 8.89%. , more than 1000 times higher than the natural mutation frequency; the mutation types are mainly manifested as leaf color mutation, leaf shape mutation and plant type mutation, of which 6.47% of the plants show bright green leaves on the leaves, 7.11% show yellow stripes, and 1.96% The leaf margins are curled, and 4.50% are short leaves, which shows that the range of artificial mutagenesis is wide and the variation types are rich.

Description

Technical field [0001] The present invention relates to the field of plant cultivation SIT polyploid mutagenesis, chemical mutagenesis particularly relates to a method of inducing mutations increase Fenjiao range. Background technique [0002] Bananas are typical of tropical and subtropical fruit trees, widely distributed in tropical and southern subtropical regions. my country is one of the main banana producing countries, growing region located in Guangdong, Hainan, Guangxi, Fujian, Yunnan and Taiwan provinces. But now, a small space for development of new varieties of banana cultivation by means of hybrid varieties of resistance is not comprehensive, and a small range of variation through natural mutation difficult emergence of new mutant gene, and is currently mutagens banana tolerance different, while having a single mutation in one aspect a method of high frequency of mutation, but the mutation rate is still low overall traits, resulting in less variation types, mutation br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 李敬阳许竹叶魏卿王安邦李羽佳王笑一许奕
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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