Primer set for enriching thalassemia genes through long-fragment PCR and application thereof

Abstract

The invention discloses a primer set for enriching thalassemia genes through long-fragment PCR and application thereof. The inventor obtained experimental data of optimized primer set for enriching the thalassemia gene by long-fragment PCR through a self-owned technology, which shows that the primer has a very good amplification effect, and the required long-fragment gene can be efficiently amplified, and the detection of the thalassemia is greatly simplified.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of gene amplification, in particular to a primer set for long-segment PCR enrichment of thalassemia genes and its application. Background technique [0002] Thalassemia (Thalassemia, referred to as thalassemia) is a fatal and disabled hereditary blood disease that seriously threatens human health. The common types are alpha thalassemia (α-thalassemia) and beta thalassemia (β-thalassemia) . In my country, the disease mainly occurs in most provinces south of the Yangtze River, especially in Guangxi, Guangdong and Hainan. The incidence of α-thalassemia is about 4-15%, and the incidence of β-thalassemia is about 1-6%. At present, thalassemia detection is mainly through large-scale population screening, as well as individual examinations set up in medical institutions for medical purposes. These two inspection methods are traditional methods, such as PCR electrophoresis, reve...

AI Technical Summary

Problems solved by technology

Ordinary long-segment PCR (Long-PCR) systems are difficult to amplify sequences with a GC content greater than 55%, especially sequences with high GC content above 5kb

This limits the detection of thalassemia

Images