A technique for tissue culture and rapid propagation of slash pine seedlings

A technology of tissue culture and slash pine, applied in plant regeneration, horticulture, horticultural methods, etc., can solve the problems of high cost, high requirements for site preparation, and many sowing procedures, and achieve the effect of low production cost, less investment, and stable genetic traits

Active Publication Date: 2022-01-07
江西环境工程职业学院
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Problems solved by technology

[0005] The purpose of the present invention is to provide a tissue culture rapid propagation technology for slash pine seedlings, aiming to solve the problem that the existing slash pine seedling propagation technology relies on seed sowing and seedling raising technology, which has high cost, many seeding procedures, and high requirements for soil preparation. technical problem

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  • A technique for tissue culture and rapid propagation of slash pine seedlings

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Embodiment 1

[0060] Embodiment 1, slash pine tender stem tissue culture regeneration:

[0061]In this embodiment, several years old slash pine was selected from the Wugong Mountain Forest Farm in Ji'an, Jiangxi Province, and the young stem explants were selected from the vigorously growing plants in early March to carry out tissue culture and multiplication test production. The samples were pretreated with shading before being collected, and the samples were collected after spraying carbendazim regularly for 7 days / time. After cutting off the needles from the tender stems of the explants, rinse them with running water under the tap for 1.5 hours, take them out and dry them in the air. Disinfect with 75% ethanol solution adding 2 drops of 1M hydrochloric acid solution per liter for 60 seconds and rinse with sterile water once. After absorbing the surface moisture with absorbent paper, disinfect with a bleach solution with an effective chlorine content of 1.5% + TWEEN-20 (3 drops / liter blea...

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Abstract

The invention provides a tissue culture rapid propagation technology for slash pine seedlings, which belongs to the field of plant nutrition and cultivation technology, and comprises the following steps: pretreating a mother plant, taking explants from the mother plant, and explanting the explants Disinfection of implants; induction of tender stems; multiplication of clustered lateral buds; rejuvenation culture of rootless buds; rooting culture; domestication culture; transplantation of tissue cultured seedlings. The invention provides a tissue culture rapid propagation technology for slash pine seedlings, through inducing and culturing the tender stems of slash pine, obtaining germinated seedlings, and obtaining a large number of rootless buds through the method of proliferating clustered buds. Then the rootless sprouts are rejuvenated by tissue culture, induced to take root, and can be transplanted out of the bottle after natural acclimation in the greenhouse. According to the technology, the progeny seedlings bred have stable genetics, comprehensive character expression, and excellent ornamental properties. Moreover, the technical system has low production cost, is environmentally friendly and does not pollute the environment, and has a fast mass production speed, fully realizing the technical requirements for large-scale mass production.

Description

technical field [0001] The invention belongs to the field of plant nutrition and cultivation technology, and more specifically relates to a tissue culture rapid propagation technology of slash pine seedlings. Background technique [0002] Slash pine is a good broad-spectrum landscaping tree species, suitable for subtropical climate areas with summer rain and winter drought. It has strong adaptability to temperature and can endure the absolute high temperature of 40°C and the absolute low temperature of -20°C. It grows well in neutral to strongly acidic red soil hills and iron nodule layers and sandy clay soils below 50-60 cm of topsoil, and it is especially good on the edge of low-lying swamps, hence the name, but it is also more drought-tolerant. Can thrive. It has strong wind resistance and rarely suffers damage under the attack of 11-12 typhoons. Its root system can withstand seawater irrigation. It is drought-resistant, hard-working, barren-resistant, and has good ada...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张付远李丹丹宋墩福景维杰
Owner 江西环境工程职业学院
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