Composite preparation for promoting growth of codonopsis pilosula and preparation method and application thereof

A technology of composite preparation and compound microbial agent, which is applied in the field of microbial agricultural application and compound microbial agent, can solve the problems of low planting efficiency, poor stress resistance, weak growth, etc., and achieve various growth indicators of Codonopsis pilosula, promote the improvement, Accelerated aging effect

Active Publication Date: 2021-02-12
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to: provide a compound preparation for promoting the growth of Codonopsis pilosula and its preparation method and application, so as to solve the defects of weak growth, poor stress resistance, low yield and low planting benefit of the above-mentioned existing Codonopsis pilosula due to continuous cropping

Method used

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  • Composite preparation for promoting growth of codonopsis pilosula and preparation method and application thereof
  • Composite preparation for promoting growth of codonopsis pilosula and preparation method and application thereof
  • Composite preparation for promoting growth of codonopsis pilosula and preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0038]A compound preparation for promoting the growth of Codonopsis pilosula, said compound microbial bacterial agent includes Trichoderma Longibrachiatum (Trichoderma Longibrachiatum), strain preservation number is CGMCC NO:19916, exogenous nitric oxide additive SNP, hydrogen peroxide H 2 o 2 , the viable count of Trichoderma Longibrachiatum is 1.0-1.5×10 6 cfu / mL, nitric oxide exogenous additive SNP and hydrogen peroxide H 2 o 2 The molar concentration ratio is 0.1:0.8.

[0039] A preparation method of a compound preparation for promoting the growth of Codonopsis pilosula, comprising the steps of:

[0040] Step 1, cultivating and activating Trichoderma Longibrachiatum, inoculating it in Hoagland liquid medium under aseptic conditions, and culturing it at 28±1°C for 24 hours to obtain a spore suspension;

[0041] Step 2, the spore suspension is inoculated into fresh Hoagland liquid medium with an inoculum size of 6%-10%, cultivated for 36h at 28±1°C, until the number of v...

Embodiment 2

[0045] On the basis of Example 1, the nitric oxide exogenous additive SNP and hydrogen peroxide H 2 o 2 The molar concentration ratio is 0.2:1, the preparation method, in step 1, cultivated at 28±1°C for 30h, and in step 2 at 28±1°C for 30h, the application is the same as that in Example 1.

Embodiment 3

[0047] On the basis of Example 1, the nitric oxide exogenous additive SNP and hydrogen peroxide H 2 o 2 The molar concentration ratio is 0.3: 1.2, in the preparation method step 1, cultured at 28±1°C for 36h, and in step 2 at 28±1°C for 24h, the application is the same as in Example 1.

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Abstract

The invention discloses a composite preparation for promoting the growth of codonopsis pilosula as well as a preparation method and application thereof, belongs to the technical field of microbial agricultural application, particularly relates to the technical field of composite microbial agents, and aims to overcome the defects of weak growth vigor, poor stress resistance, low yield and low planting benefit of codonopsis pilosula in the prior art due to continuous cropping. The composite preparation (namely the composite microbial agent) for promoting the growth of codonopsis pilosula comprises Trichoderma Longibrachiatum, wherein the preservation number is CGMCC NO: 19916; a nitric oxide exogenous additive SNP, and hydrogen peroxide H2O2. The composite microbial agent can obviously influence the growth and metabolism process of codonopsis pilosula, especially can synergistically improve the growth condition of codonopsis pilosula, promote the increase of chlorophyll and sugar contentof codonopsis pilosula, and enhance the stress resistance and root activity of codonopsis pilosula, and has important meanings for improving the quality and yield of codonopsis pilosula and improvingthe planting benefit.

Description

technical field [0001] A compound preparation for promoting the growth of Codonopsis pilosula, its preparation method and application, the invention belongs to the technical field of microbial agricultural application, and specifically relates to the technical field of compound microbial bacterial agents. Background technique [0002] Codonopsis Codonopsis is a herbal medicine included in the "Chinese Pharmacopoeia". The source of herbal medicine is the dried root of Codonopsis Codonopsis, Suhua Codonopsis, or Sichuan Codonopsis. Codonopsis is sweet in taste and flat in nature. It has the effects of invigorating the middle and replenishing qi, quenching thirst, invigorating the spleen and lungs, nourishing blood and promoting body fluid. It is used for deficiency of spleen and lung qi, fatigue due to lack of food, cough and shortness of breath, deficiency of qi and blood, pale complexion, palpitation, shortness of breath, thirsty body fluid wound, internal heat and quenchin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/38A01N59/24A01N59/00A01P21/00C12N1/14C12R1/885
CPCA01N63/38A01N59/24A01N59/00C12N1/14
Inventor 王永刚王江勤晏祖花李渊利陈凯丁苗胡舒冷非凡马建忠陈吉祥
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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