Chinese herbal medicine feed additive for resisting nitrite nitrogen stress for penaeus vannamei boone and preparation method of Chinese herbal medicine feed additive
A technology of feed additive and Chinese herbal medicine, which is applied in the field of Chinese herbal medicine feed additive and its preparation for Penaeus vannamei against nitrite nitrogen stress, which can solve the problems of farmers’ loss, impact on the immunity of Penaeus vannamei, and the reduction of immunity
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Embodiment 1
[0019] Recipe: 3 parts of alcohol extract concentrate of asparagus decoction pieces, 2 parts of Jiaogulan powder, and 1 part of licorice powder.
[0020] Preparation method: mix the ethanol extract concentrate of asparagus decoction pieces, Gynostemma pentaphylla powder, and licorice powder in proportion, add an appropriate amount of 80% food-grade ethanol solution to dissolve, and stir to form a medicinal puree; put the medicinal puree into a granulator and mix evenly, and then Dry in an oven at a constant temperature of 75 degrees, pulverize with a Chinese herbal medicine pulverizer, sieve through 80 meshes, pack into bags (1kg / bag), and store in a sealed place in a cool place.
[0021] Wherein, the ethanol extract concentrate of the asparagus decoction pieces is prepared as follows: put the asparagus decoction pieces into a water-proof heating pot, add a 15% ethanol solution with a concentration 9 times the mass of the asparagus decoction pieces, and then add 1mol / L NaOH sol...
Embodiment 2
[0023] Recipe: 5 parts of alcohol extract concentrate of asparagus decoction pieces, 4 parts of Jiaogulan powder, and 2 parts of licorice powder.
[0024] Preparation method: with embodiment 1
[0025] Wherein, the alcohol extract concentrate of Asparagus decoction pieces is prepared as follows: put the Asparagus decoction pieces into a water-proof heating pot, add 25% ethanol solution with 12 times the mass of Asparagus decoction pieces, and then add 1mol / L NaOH solution to adjust the pH to 11, after soaking for 1.5 hours, use 1mol / L NaOH solution to adjust the pH to 11; then, extract at 80°C for 3 hours, filter with gauze, and extract the filtrate again, repeat twice, combine the extracts three times, heat and concentrate under reduced pressure To a density of 1.18g / ml (crude drug concentration 1g / mL), keep it for later use.
Embodiment 3
[0027] Recipe: 8 parts of alcohol extract concentrate of asparagus decoction pieces, 6 parts of Gynostemma pentaphyllum powder, and 3 parts of licorice powder.
[0028] Preparation method: with embodiment 1
[0029] Wherein, the alcohol extract concentrate of the asparagus decoction pieces is prepared as follows: put the asparagus decoction pieces into a water-proof heating pot, add a 35% ethanol solution with a concentration 15 times the mass of the asparagus decoction pieces, and then add 1mol / L NaOH solution to adjust the pH to 13, after soaking for 2.5 hours, use 1mol / L NaOH solution to adjust the pH to 13; then, extract in water at 95°C for 4.5 hours, filter with gauze, extract the filtrate again, repeat twice, combine the extracts three times, and heat under reduced pressure Concentrate to a density of 1.24g / ml (crude drug concentration 1g / mL), and keep for future use.
[0030] 2. Research on anti-nitrite nitrogen stress and Chinese herbal medicine feed additive for Pen...
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