Chinese herbal medicine feed additive for resisting nitrite nitrogen stress for penaeus vannamei boone and preparation method of Chinese herbal medicine feed additive

A technology of feed additive and Chinese herbal medicine, which is applied in the field of Chinese herbal medicine feed additive and its preparation for Penaeus vannamei against nitrite nitrogen stress, which can solve the problems of farmers’ loss, impact on the immunity of Penaeus vannamei, and the reduction of immunity

Pending Publication Date: 2022-04-15
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor fluidity of the aquaculture water, the bottom of the pond is mostly in an anoxic state. After the fermentation and decomposition of anaerobic bacteria in the water, the ammonia and nitrite content in the bottom water often exceed the tolerance value of prawns. The living habits of the bottom will cause its environmental stress, which will seriously affect the immunity of Penaeus vannamei
The immune system of prawns is simple and sensitive to stress. Under stress, the immunity is greatly reduced. Pathogens in the water and environmental factors plus the pathogenic microorganisms of prawns will take advantage of the emptiness. Once handled improperly, it will cause farmers incalculable loss

Method used

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  • Chinese herbal medicine feed additive for resisting nitrite nitrogen stress for penaeus vannamei boone and preparation method of Chinese herbal medicine feed additive
  • Chinese herbal medicine feed additive for resisting nitrite nitrogen stress for penaeus vannamei boone and preparation method of Chinese herbal medicine feed additive
  • Chinese herbal medicine feed additive for resisting nitrite nitrogen stress for penaeus vannamei boone and preparation method of Chinese herbal medicine feed additive

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Experimental program
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Effect test

Embodiment 1

[0019] Recipe: 3 parts of alcohol extract concentrate of asparagus decoction pieces, 2 parts of Jiaogulan powder, and 1 part of licorice powder.

[0020] Preparation method: mix the ethanol extract concentrate of asparagus decoction pieces, Gynostemma pentaphylla powder, and licorice powder in proportion, add an appropriate amount of 80% food-grade ethanol solution to dissolve, and stir to form a medicinal puree; put the medicinal puree into a granulator and mix evenly, and then Dry in an oven at a constant temperature of 75 degrees, pulverize with a Chinese herbal medicine pulverizer, sieve through 80 meshes, pack into bags (1kg / bag), and store in a sealed place in a cool place.

[0021] Wherein, the ethanol extract concentrate of the asparagus decoction pieces is prepared as follows: put the asparagus decoction pieces into a water-proof heating pot, add a 15% ethanol solution with a concentration 9 times the mass of the asparagus decoction pieces, and then add 1mol / L NaOH sol...

Embodiment 2

[0023] Recipe: 5 parts of alcohol extract concentrate of asparagus decoction pieces, 4 parts of Jiaogulan powder, and 2 parts of licorice powder.

[0024] Preparation method: with embodiment 1

[0025] Wherein, the alcohol extract concentrate of Asparagus decoction pieces is prepared as follows: put the Asparagus decoction pieces into a water-proof heating pot, add 25% ethanol solution with 12 times the mass of Asparagus decoction pieces, and then add 1mol / L NaOH solution to adjust the pH to 11, after soaking for 1.5 hours, use 1mol / L NaOH solution to adjust the pH to 11; then, extract at 80°C for 3 hours, filter with gauze, and extract the filtrate again, repeat twice, combine the extracts three times, heat and concentrate under reduced pressure To a density of 1.18g / ml (crude drug concentration 1g / mL), keep it for later use.

Embodiment 3

[0027] Recipe: 8 parts of alcohol extract concentrate of asparagus decoction pieces, 6 parts of Gynostemma pentaphyllum powder, and 3 parts of licorice powder.

[0028] Preparation method: with embodiment 1

[0029] Wherein, the alcohol extract concentrate of the asparagus decoction pieces is prepared as follows: put the asparagus decoction pieces into a water-proof heating pot, add a 35% ethanol solution with a concentration 15 times the mass of the asparagus decoction pieces, and then add 1mol / L NaOH solution to adjust the pH to 13, after soaking for 2.5 hours, use 1mol / L NaOH solution to adjust the pH to 13; then, extract in water at 95°C for 4.5 hours, filter with gauze, extract the filtrate again, repeat twice, combine the extracts three times, and heat under reduced pressure Concentrate to a density of 1.24g / ml (crude drug concentration 1g / mL), and keep for future use.

[0030] 2. Research on anti-nitrite nitrogen stress and Chinese herbal medicine feed additive for Pen...

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Abstract

The invention discloses a Chinese herbal medicine feed additive for resisting nitrite nitrogen stress for penaeus vannamei. The Chinese herbal medicine feed additive is prepared from radix asparagi, fiveleaf gynostemma herb and liquorice. Therefore, a corresponding preparation method is also established, namely the radix asparagi decoction pieces are extracted by using ethanol in an alkaline environment, the extracting solution is concentrated under reduced pressure for later use, then a certain amount of gynostemma pentaphyllum powder, licorice root powder and food-grade ethanol are gradually added, and the mixture is uniformly stirred, dried, crushed and sieved by eight sieves. The obtained Chinese herbal medicine feed additive has the characteristics of being pure natural, easy to prepare, low in toxicity, low in cost, high in effective component content and the like, has the effects of adjusting the immunity of the penaeus vannamei, resisting nitrite nitrogen stress and promoting growth, effectively improves the anti-stress capability and growth indexes of the penaeus vannamei, is relatively small in side effect, can effectively avoid drug resistance generated by pathogens, and has a good application prospect. The feed additive is a natural and green feed additive.

Description

technical field [0001] The invention belongs to the field of culture technology of Penaeus vannamei, and in particular relates to a Chinese herbal medicine feed additive for resisting nitrous nitrogen stress for Penaeus vannamei and a preparation method thereof. Background technique [0002] Vannamei (Litopenaeus vannamei), because of its excellent environmental adaptability and tolerance, makes it stand out in the artificial culture of shrimp, and it is also one of the three excellent species with the highest breeding output in the world. China once introduced Penaeus vannamei in the late 1980s, and artificially bred it successfully in the early 1990s. Since the introduction, it has been widely cultivated in China and has become the main target of shrimp farming in China. [0003] During the breeding process of Penaeus vannamei, with the extension of the culture time, a large amount of shrimp excrement and residual bait that cannot be caught in time will be deposited on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/30A23K40/10A01K61/59B01D11/02
CPCY02A40/81Y02A40/818
Inventor 胡庭俊孙钰博谢小东王良刚曹永强季露张文赵怡陈奇韦英益于美玲
Owner GUANGXI UNIV
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