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Microparticle measurement spectrometer, microparticle measurement device using microparticle measurement spectrometer, and method for correcting microparticle measurement photoelectric conversion system

A technology of photoelectric conversion and spectrometer, applied in spectrometry/spectrophotometry/monochromator, measuring device, fluorescence/phosphorescence, etc., which can solve problems such as errors and repeated use of reference spectra

Pending Publication Date: 2021-02-12
SONY CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the uniformity data for each product is a value under different measurement conditions, not when incorporated and used in a measurement device such as a flow cytometer, and not within the wavelength range that each channel is responsible for , so an error occurs, and it is not feasible to reuse a reference spectrum just by performing this correction

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  • Microparticle measurement spectrometer, microparticle measurement device using microparticle measurement spectrometer, and method for correcting microparticle measurement photoelectric conversion system
  • Microparticle measurement spectrometer, microparticle measurement device using microparticle measurement spectrometer, and method for correcting microparticle measurement photoelectric conversion system
  • Microparticle measurement spectrometer, microparticle measurement device using microparticle measurement spectrometer, and method for correcting microparticle measurement photoelectric conversion system

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[0083] The method for calibrating a microparticle measurement photoelectric conversion system according to the present technology is a method for calibrating a microparticle measurement photoelectric conversion system in which light emitted from microparticles flowing through a flow path is detected by a plurality of light receiving elements having different detection wavelength ranges received, and the light information obtained by the light-receiving element is converted into electrical information, characterized in that when light with the same amount of light per unit wavelength is incident, the output of all channels is uniform.

[0084] Combine below figure 1 and figure 2 A description will be given of a specific example in which the microparticle measurement photoelectric conversion system is corrected so that the outputs of all the channels become uniform when light having the same amount of light per unit wavelength is incident. figure 1 is a conceptual diagram show...

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Abstract

Provided is technology that enables quantitative data to be handled, without a degradation in SNR, in a spectrum-type microparticle measurement device. The present technology provides a microparticlemeasurement spectrometer that is provided with: a spectroscopic element for dispersing light emitted from microparticles flowing through a flow channel; and a photoelectric conversion array which hasa plurality of light-receiving elements of different detection wavelength regions and converts optical information, obtained from the light-receiving elements, into electrical information, wherein theoutput of all channels is uniform in the photoelectric conversion array when light having the same light quantity per unit wavelength is incident thereon.

Description

technical field [0001] This technology relates to particle measurement spectrometers. More specifically, the present technology relates to a particle measurement spectrometer for detecting optical information of particles flowing in a flow path, a particle measurement device using the particle measurement spectrometer, and a method for calibrating a particle measurement photoelectric conversion system. Background technique [0002] In recent years, with the development of analytical methods, a method has been developed in which fine particles such as biological fine particles (eg, cells and microorganisms, microbeads, etc.) The measured particles are analyzed and classified. [0003] As a typical example of such a method for analyzing or classifying microparticles, technological improvement of an analysis method called flow cytometry has been rapidly developing. Flow cytometry is an analysis method in which microparticles to be analyzed are poured into a fluid in an aligne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01J3/02G01N21/64
CPCG01J3/28G01N15/1434G01N15/1459G01N2015/1493G01N2015/1497G01N15/0205G01N2015/0294G01N2015/1006G01N15/149G01J3/02G01N15/1012G01N15/1429G01N21/64
Inventor 原雅明梅津友行冈本好喜
Owner SONY CORP