A biological detection method based on excitation light source

Abstract

The invention provides a biological detection method based on an excitation light source, which injects a sample into a sample hole arranged on the sample layer; At this time, the gasket is pulled out along the track, and the sample loading layer is pressed down, so that the pipeline layer and the sample loading layer are clamped, and a row of puncture needles arranged on the pipeline layer are mixed with the reagents of the sample loading layer in sequence, After extraction and purification reactions occur in the pipeline layer, the nucleic acid material in the sample is introduced into the amplification chamber provided on the pipeline layer; the excitation light module emits excitation light with a preset wavelength to the amplification chamber, and after the reaction occurs, the The fluorescence sensor obtains the fluorescence information to complete the detection, and the temperature control module controls the temperature in the amplification chamber to be within a preset range, so that the reagents of the chip device can achieve an optimal reaction state.

Description

technical field [0001] The invention relates to the technical field of nucleic acid amplification instrument detection, in particular to a biological detection method based on an excitation light source. Background technique [0002] In recent years, fluorescence analysis technology has been widely used in fluorescence microscopy imaging and observation, and flow fluorescence detection and analysis. The required fluorescence spectrophotometer includes an excitation light device that inputs excitation energy, that is, an excitation light source. At present, fluorescence microscopy imaging systems generally use mercury lamps, xenon lamps and other high-energy, short-life full-band light sources, which are filtered by different filters to obtain excitation light sources of the corresponding required bands. However, the optical path system of this kind of excitation light source is complicated, the energy consumption of the light source is high, the efficiency loss is large, and...

AI Technical Summary

Problems solved by technology

[0004] In the prior art, some fluorescence detection achieves the purpose of multi-light path detection by adding a rotatable excitation light filter color wheel and an emission light color wheel in the optical path, but the setting of the above structure increases power consumption, and, in the fluorescence detection process In the process, due to the mechanical rotation, there are problems of misalignment or misalignment of light, and the instrument is large and clumsy, not easy to carry, and it will affect the progress of the experiment in the process of multiple corrections

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