Method for determining collapse temperature of freeze-dried material by utilizing image processing technology

Abstract

The invention discloses a method for determining collapse temperature of a freeze-dried material by utilizing an image processing technology. The method comprises the following steps: S1, obtaining animage of the freeze-dried material in a heating process through a freeze-drying microscope; S2, selecting an appropriate threshold T for the image number obtained by the freeze-drying microscope, andconverting the grayscale image into a binary image according to the threshold T; S3, judging whether the threshold T in the step S2 is reasonable or not, returning to the step S2 if the threshold T is not reasonable, and executing the next step if the threshold T is reasonable; S4, cutting the black edge information of the lower part of the image obtained in the step S2; S5, cutting the image obtained in the step S4; S6, running an area filtering program; S7, judging whether the area filtering value in the step S6 is reasonable or not; and S8, filtering points with relatively small areas in the image obtained in the step S6. According to the invention, accurate collapse temperature data with good stability are obtained through the image processing technology, and data errors caused by subjective ideas are avoided.

Description

technical field [0001] The invention relates to the technical field of determining the collapse temperature of freeze-dried materials, in particular to a method for determining the collapse temperature of freeze-dried materials by using image processing technology. Background technique [0002] Freeze-drying is a widely used preservation method for biological products. During freeze-drying, when the temperature of the drying layer rises to a certain value, the ice crystals in the material disappear, and the space originally occupied by the ice crystals becomes a cavity, so the freeze-dried layer has a porous honeycomb sponge structure. This structure is temperature dependent. When the temperature of the solid matrix of the honeycomb structure is high, its rigidity decreases. When the temperature reaches a certain critical value, the rigidity of the solid matrix is ​​not enough to maintain the honeycomb structure, the solid matrix wall of the cavity will collapse, and the o...

AI Technical Summary

Problems solved by technology

However, this method is highly subjective, and repeated experiments may lead to fuzzy experimental results, resulting in inaccurate experimental results.

After being applied to the actual freeze-drying, the quality of the product will decrease

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