Biosynthesis and purification method of silver selenide quantum dots

Abstract

The invention discloses a biosynthesis and purification method of silver selenide quantum dots, and belongs to the technical field of microbiology and nanotechnology. According to the biosynthesis andpurification method of the silver selenide quantum dot, silver nitrate is used as a silver source, selenium-enriched yeast is used as a synthesis carrier, culture is carried out for 10-100 h at the temperature of 30 DEG C and the speed of 200 r / min, centrifugation is carried out, thalli are removed, a supernate is collected, ultrafiltration is carried out on the supernate through an ultrafiltration tube, and finally the purified Ag2Se quantum dot is obtained. According to the method, the Ag2Se quantum dot which is about 3.9 nm in size, low in toxicity and good in water solubility is synthesized, and a lattice structure can be clearly seen under a high-resolution transmission electron microscope. According to the invention, Ag2Se quantum dots are synthesized in saccharomyces cerevisiae forthe first time, the synthesis method is friendly to conditions, strict synthesis conditions such as high temperature, high pressure and the like are not involved, and the purification method is simple to operate and has relatively high application value.

Description

technical field [0001] The invention belongs to the field of microbiology and nanotechnology, and in particular relates to a biosynthesis and purification method of silver selenide quantum dots. Background technique [0002] Because nanotechnology can be applied in many fields, nanomaterials are the basis of nanotechnology, and the use of low-toxic nanomaterials for biological imaging and biological detection has attracted much attention. Nanomaterials that can emit near-infrared fluorescence can reduce the impact of biological autofluorescence on biological imaging. Therefore, the synthesis of high-performance near-infrared fluorescent nanomaterials is particularly important. [0003] The traditional quantum dot synthesis method is chemical synthesis, which often involves toxic and harmful reagents, the synthesis is not easy to control, and the separation of by-products is difficult. Biosynthesis and traditional chemical synthesis have mild conditions, but most of them are ...

AI Technical Summary

Problems solved by technology

[0003] The traditional quantum dot synthesis method is chemical synthesis, which often involves toxic and harmful reagents, the synthesis is not easy to control, and the separation of by-products is difficult. Biosynthesis and traditional chemical synthesis have mild conditions, but most of them are intracellular synthesis, and extraction and purification are more difficult. , this feature severely limits the industrial production of biosynthetic nanomaterials

In addition, most of the synthesized nanomaterials are nanomaterials that emit visible light. At present, there are not many materials that can emit near-infrared fluorescence, and there are few synthesis methods and complicated purification methods. These characteristics limit the application of biosynthetic nanomaterials in biological imaging and biological detection applications

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