Biosynthesis and purification method of silver selenide quantum dots

A technology of biosynthesis and purification methods, applied in the field of biosynthesis and purification of silver selenide quantum dots, can solve the problems of few synthesis methods, difficult extraction and purification, complicated purification methods, etc., achieve good water solubility, simple extraction and purification steps, The effect of low toxicity
CN112430463AActive Publication Date: 2021-03-02WUHAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN UNIV
Publication Date
2021-03-02

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Abstract

The invention discloses a biosynthesis and purification method of silver selenide quantum dots, and belongs to the technical field of microbiology and nanotechnology. According to the biosynthesis andpurification method of the silver selenide quantum dot, silver nitrate is used as a silver source, selenium-enriched yeast is used as a synthesis carrier, culture is carried out for 10-100 h at the temperature of 30 DEG C and the speed of 200 r / min, centrifugation is carried out, thalli are removed, a supernate is collected, ultrafiltration is carried out on the supernate through an ultrafiltration tube, and finally the purified Ag2Se quantum dot is obtained. According to the method, the Ag2Se quantum dot which is about 3.9 nm in size, low in toxicity and good in water solubility is synthesized, and a lattice structure can be clearly seen under a high-resolution transmission electron microscope. According to the invention, Ag2Se quantum dots are synthesized in saccharomyces cerevisiae forthe first time, the synthesis method is friendly to conditions, strict synthesis conditions such as high temperature, high pressure and the like are not involved, and the purification method is simple to operate and has relatively high application value.
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Description

technical field

[0001] The invention belongs to the field of microbiology and nanotechnology, and in particular relates to a biosynthesis and purification method of silver selenide quantum dots. Background technique

[0002] Because nanotechnology can be applied in many fields, nanomaterials are the basis of nanotechnology, and the use of low-toxic nanomaterials for biological imaging and biological detection has attracted much attention. Nanomaterials that can emit near-infrared fluorescence can reduce the impact of biological autofluorescence on biological imaging. Therefore, the synthesis of high-performance near-infrared fluorescent nanomaterials is particularly important.

[0003] The traditional quantum dot synthesis method is chemical synthesis, which often involves toxic and harmful reagents, the synthesis is not easy to control, and the separation of by-products is difficult. Biosynthesis and traditional chemical synthesis have mild conditions, but most of them are ...

Claims

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