Biosynthesis and purification method of silver selenide quantum dots

Abstract

The invention discloses a biosynthesis and purification method of silver selenide quantum dots, belonging to the fields of microbiology and nanotechnology. The biosynthesis and purification method of silver selenide quantum dots of the present invention uses silver nitrate as the silver source and selenium-enriched yeast as the synthesis carrier, cultures at 30°C and 200r / min for 10-100h, centrifuges, removes the bacteria, collects the supernatant, The supernatant was ultrafiltered with an ultrafiltration tube, and finally the purified Ag was obtained 2 Se quantum dots. The method of the present invention has synthesized Ag with a size of about 3.9nm, low toxicity and good water solubility. 2 Se quantum dots, the lattice structure can be clearly seen under the high-resolution transmission electron microscope. The present invention synthesized Ag in Saccharomyces cerevisiae for the first time 2 Se quantum dots, and the synthesis method has friendly conditions, does not involve harsh synthesis conditions such as high temperature and high pressure, and the purification method is simple to operate and has high application value.

Description

technical field [0001] The invention belongs to the field of microbiology and nanotechnology, and in particular relates to a biosynthesis and purification method of silver selenide quantum dots. Background technique [0002] Because nanotechnology can be applied in many fields, nanomaterials are the basis of nanotechnology, and the use of low-toxic nanomaterials for biological imaging and biological detection has attracted much attention. Nanomaterials that can emit near-infrared fluorescence can reduce the impact of biological autofluorescence on biological imaging. Therefore, the synthesis of high-performance near-infrared fluorescent nanomaterials is particularly important. [0003] The traditional quantum dot synthesis method is chemical synthesis, which often involves toxic and harmful reagents, the synthesis is not easy to control, and the separation of by-products is difficult. Biosynthesis and traditional chemical synthesis have mild conditions, but most of them are ...

AI Technical Summary

Problems solved by technology

[0003] The traditional quantum dot synthesis method is chemical synthesis, which often involves toxic and harmful reagents, the synthesis is not easy to control, and the separation of by-products is difficult. Biosynthesis and traditional chemical synthesis have mild conditions, but most of them are intracellular synthesis, and extraction and purification are more difficult. , this feature severely limits the industrial production of biosynthetic nanomaterials

In addition, most of the synthesized nanomaterials are nanomaterials that emit visible light. At present, there are not many materials that can emit near-infrared fluorescence, and there are few synthesis methods and complicated purification methods. These characteristics limit the application of biosynthetic nanomaterials in biological imaging and biological detection applications

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