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Continuous cropping resistant microbial inoculum containing filamentous bacillus and application thereof

A technology of bacillus and stubble bacteria agent, applied in the direction of application, bacteria, organic fertilizer, etc., can solve the problems of compaction, enhanced drug resistance of pathogenic bacteria, secondary salinization and other problems that cannot be solved well at the same time, and achieve soil micro Excellent shape and structure, promoting growth and increasing plant height

Active Publication Date: 2021-03-05
QINGDAO SHANGDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent decades, in order to develop the agricultural industry and increase unit output, people have repeatedly planted similar economic crops all year round. Over time, the problem of repeated crop crops has become increasingly prominent; in addition, people have used a large amount of chemical fertilizers for a long time, which in turn has brought about soil compaction and secondary growth. Salinization, nutritional imbalance and other problems, and the long-term abuse of pesticides has also led to serious prob

Method used

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  • Continuous cropping resistant microbial inoculum containing filamentous bacillus and application thereof
  • Continuous cropping resistant microbial inoculum containing filamentous bacillus and application thereof
  • Continuous cropping resistant microbial inoculum containing filamentous bacillus and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Isolation, screening and identification of Bacillus filamentum GBW-F006

[0034] 1. Isolation and Screening of Filamentous Bacillus GBW-F006

[0035] Crop rhizosphere soils were taken from perennial vegetable greenhouses in Cangzhou, Handan, Henan, Puyang, Anyang, Qingdao, Haiyang, Qingzhou, and Shouguang in Hebei, stored in a medical test sample bag at 4°C, and separated in time. 56 kinds of crops including pepper, zucchini, cauliflower, melon, tomato and cucumber. Take 2g of soil sample, put it into 18mL sterile water, shake and extract at 30°C and 200rpm / min for 30min, put the sample solution in a water bath at 75°C for 20min, and then carry out gradient dilution, take 100μl of each gradient dilution solution and spread it on the NA plate. Incubate the coated plate upside down at 37°C for 48 hours, pick out single colonies of different shapes with sterilized bamboo sticks, transfer them to the NA slope, culture at 37°C for 48 hours, and confirm the spore ...

Embodiment 2

[0044] Embodiment 2: the preparation of anti-cropping bacterial agent

[0045] The preparation method of anti-cropping bacterial agent of the present invention is as follows:

[0046] 1. Put Bacillus filamentous bacteria GBW-F006 in the seed culture solution (the formula is: 1-1.5g corn steep liquor powder, 2-2.5g cottonseed protein powder, 0.8-1g peptone, 0.5-0.8g yeast extract, KH 2 PO 4 0.1~0.3g, MgSO4 7H 2 O 0.05~0.08g, MnSO 4 ·H 2 O 0.03~0.05g, neutral protease 50,000 U / g 0.1~0.2g, pH 7.3~7.5, distilled water 1L) and culture at 35℃~38℃ for 12-14h at constant temperature to obtain GBW-F006 seed solution;

[0047] 2. Inoculate the GBW-F006 seed liquid into the expansion culture medium according to the inoculation dose of 5-8% (the formula is: krill powder 8-10g, molasses 5-6g, ammonium chloride 2-3g, sodium citrate 1- 1.5g, manganese chloride 0.5~0.8g, magnesium sulfate 0.2~0.3g, disodium hydrogen phosphate 0.15~0.25g, potassium dihydrogen phosphate 0.25~0.35g, pH 7.6...

Embodiment 3

[0053] The experiment was carried out in a tomato planting base in the Shouguang rice field, where tomatoes are grown all year round, and the phenomenon of repeated cropping is typical; the experiments were carried out in three different tomato planting greenhouses, No. 1, No. 2 and No. 3. Three greenhouses were set up with control group and treatment group respectively, which were divided into treatment 1 and control 1 (T1 and CK1), treatment 2 and control 2 (T2 and CK2), treatment 3 and control 3 (T3 and CK3), each greenhouse Select the soil in the middle position for the test, the land area of ​​the treatment group and the control group are 50m each 2 , adopt the method of rotary tillage to prepare the soil, and apply the anti-stubble bacterial agent prepared in Example 2 on the land of the treatment group with the application dose of 80kg / mu in the three greenhouses, and then use the rotary tiller to plow the ground.

[0054] The test lasted for 10 days, focusing on the ef...

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Abstract

The invention discloses a continuous cropping resistant microbial inoculum containing filamentous bacillus and application thereof. The continuous cropping resistant microbial inoculum comprises filamentous bacillus powder with the microbial content not lower than 2.0 * 10<8>CFU/g and a compound carrier, wherein the mass ratio of the filamentous bacillus powder to the compound carrier is 1: (350-700); the filamentous bacillus powder is prepared from filamentous bacillus GBW-F006 with the preservation number of CGMCC No. 20918; and the compound carrier is prepared by mixing, fermenting and composting a carrier matrix and mushroom residues. The continuous cropping resistant microbial inoculum can be used for improving soil of a multiple cropping and continuous cropping facility, improving the micro-ecological structure of the soil and increasing the volume weight of the soil, so that the plant height, stem diameter and yield of crops are increased, the planting economic benefits of the crops are increased, and the continuous cropping resistant microbial inoculum has a wide application prospect.

Description

technical field [0001] The invention relates to the field of plant planting, in particular to an anti-cropping bacterial agent containing Bacillus filamentous and its application. Background technique [0002] Continuous cropping (continuous cropping obstacle) refers to the continuous planting of the same crop or related crops for multiple crops, even under normal management conditions, it will lead to poor growth and development of crops, reduced yield, poor quality, and increased soil-borne diseases and insect pests. Long-term cropping cultivation can cause crops to over-consume some nutrients in the soil and cause some nutrients to be deficient. The crops will have physiological diseases due to the lack of these over-consumed nutrients. In recent decades, in order to develop the agricultural industry and increase unit output, people have repeatedly planted similar economic crops all year round. Over time, the problem of repeated crop crops has become increasingly prominen...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/60C05G3/80C05F17/20C12R1/07
CPCC12N1/20C05F3/00C05F17/20C05G3/00C05G3/60C05G3/80C05F5/00C05F11/08Y02W30/40Y02P60/21
Inventor 魏秉培王金龙李慧芬张大伟樊连梅陈四明朱玉翠
Owner QINGDAO SHANGDE BIOTECH
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