Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Drug rash plasma exosome protein and kit thereof

A technology of exosomes and proteins, applied in the direction of keratin, muscle protein, cytokeratin, etc., can solve the problems of subsequent analysis interference of exosomes, and achieve the effect of short extraction time, high purity and efficient extraction

Pending Publication Date: 2021-03-12
AIR FORCE MEDICAL UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current commonly used exosome extraction methods cannot meet the requirements of specific extraction and analysis of tumor cell exosomes in actual samples, thus causing interference to the subsequent analysis of exosomes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: A drug eruption plasma exosomal protein, including protein Galectin-7, protein ALG-2, protein MPO, protein HSP90, protein CALML5, protein LCN-2, protein CCAR2, protein FTH1, protein CTSG, protein CRP, One or more of keratin, actin, histone, myeloperoxidase, matrix metalloproteinase-9, inflammatory protein.

[0018] Specifically, the combination of protein Galectin-7, protein ALG-2 and protein LCN-2 is included.

[0019] Specifically, it includes the combination of protein FTH1, protein CTSG, keratin, actin and histone.

[0020] Specifically, the method for extracting exosome proteins is as follows: adding the extraction material to the liquid to be extracted containing exosomes to obtain an extraction mixture;

[0021] The extraction mixture is centrifuged to make the exosomes enter into the precipitate, the precipitate is collected, and the extraction material in the precipitate is removed to obtain the exosome.

[0022] Specifically, the extraction materi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a drug rash plasma exosome protein, which comprises one or more of protein Galectin-7, protein ALG-2, protein MPO, protein HSP90, protein CALML5, protein LCN2, protein CCAR2, protein FTH1, protein CTSG, protein CRP, keratin, actin, histone, myeloperoxidase, matrix metalloproteinase 9 and inflammatory protein. According to the exosome extraction method, efficient extractionof culture products of human body fluid or cells can be achieved, the extraction time is short, and the purity of the obtained exosome is high; besides, the exosome can be used as a biomarker of the drug rash, can detect whether the drug rash exists or not, and can also be used for early warning and intervention to evaluate the curative effect of the drug.

Description

technical field [0001] The invention belongs to the technical field of plasma exosomes, and more specifically relates to a drug eruption plasma exosome protein. Background technique [0002] Drug eruption, also known as drug-induced dermatitis, is a reaction to inflammation of the skin and mucous membranes caused by drugs entering the human body through oral, external, and injection routes. Drug-induced adverse reactions are very complex and can be roughly divided into: drug overdose, intolerance, idiopathic, side effects, secondary effects, and allergic reactions. Drug eruptions are the most common type of allergic reaction. [0003] Exosomes exist not only in cell culture fluid, but also in body fluids such as blood, urine, amniotic fluid, breast milk, semen, saliva, and pleural effusion. Their components include lipid bilayers and cell-derived proteins and RNAs. , DNA and other substances. Almost all cells can produce exosomes, and exosomes from different cell sources ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883C07K14/47C12N9/08C12N9/64C07K1/14
CPCC12Q1/6883C07K14/47C07K14/4716C07K14/4728C07K14/4741C12N9/0065C12N9/6491C12Y111/02002C12Y304/24035C12Q2600/106
Inventor 付萌张晨乔洪江党二乐
Owner AIR FORCE MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com