Drug rash plasma exosome protein and kit thereof

Abstract

The invention discloses a drug rash plasma exosome protein, which comprises one or more of protein Galectin-7, protein ALG-2, protein MPO, protein HSP90, protein CALML5, protein LCN2, protein CCAR2, protein FTH1, protein CTSG, protein CRP, keratin, actin, histone, myeloperoxidase, matrix metalloproteinase 9 and inflammatory protein. According to the exosome extraction method, efficient extractionof culture products of human body fluid or cells can be achieved, the extraction time is short, and the purity of the obtained exosome is high; besides, the exosome can be used as a biomarker of the drug rash, can detect whether the drug rash exists or not, and can also be used for early warning and intervention to evaluate the curative effect of the drug.

Description

technical field [0001] The invention belongs to the technical field of plasma exosomes, and more specifically relates to a drug eruption plasma exosome protein. Background technique [0002] Drug eruption, also known as drug-induced dermatitis, is a reaction to inflammation of the skin and mucous membranes caused by drugs entering the human body through oral, external, and injection routes. Drug-induced adverse reactions are very complex and can be roughly divided into: drug overdose, intolerance, idiopathic, side effects, secondary effects, and allergic reactions. Drug eruptions are the most common type of allergic reaction. [0003] Exosomes exist not only in cell culture fluid, but also in body fluids such as blood, urine, amniotic fluid, breast milk, semen, saliva, and pleural effusion. Their components include lipid bilayers and cell-derived proteins and RNAs. , DNA and other substances. Almost all cells can produce exosomes, and exosomes from different cell sources ...

AI Technical Summary

Problems solved by technology

[0004] The current commonly used exosome extraction methods cannot meet the requirements of specific extraction and