Method for rapidly obtaining brood amount of euphausidae shrimps

A quick, shrimp-like technique

Pending Publication Date: 2021-03-16
MARINE FISHERIES RES INST OF ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This measurement method still has the following defects: time-consuming and labor-intensive, great workload, and low measurement efficiency

Method used

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  • Method for rapidly obtaining brood amount of euphausidae shrimps

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Sampling and weighing: After thawing the long-armed shrimp frozen at -80°C, the whole shrimp was weighed first, and the weight was 7.5035g (denoted as W 1 ); then the shrimp roe of the shrimp abdomen is brushed down with a fiber brush and then collected with a centrifuge tube, and the shrimp after removing the shrimp roe is weighed, and the weight is 4.7958g (recorded as W 2 );

[0024] (2) Dispersion and constant volume: add 10mL dispersion liquid (8% formalin) to the shrimp eggs and soak for 20 minutes, ultrasonically clean for 1 minute, remove the floating matter on the surface of the liquid, wash the shrimp eggs with distilled water for 3 times, and then wash the shrimp eggs with distilled water. Eggs were put into a graduated cylinder, and distilled water was added to set the volume to 50mL to obtain a shrimp egg dispersion (the total volume of the shrimp egg dispersion was denoted as V 0 );

[0025] (3) Counting: use a pipette gun to draw 3mL of the shrimp e...

Embodiment 2

[0029] (1) Sampling and weighing: After thawing the white prawns frozen at -80°C, weigh the whole prawns first, and the weight is 5.2125g (denoted as W 1 ); then the shrimp roe of the shrimp abdomen is brushed down with a fiber brush and then collected with a centrifuge tube, and the shrimp after removing the shrimp roe is weighed, and the weight is 3.7057g (recorded as W 2 );

[0030] (2) Dispersion and constant volume: add 15mL dispersion solution (8% formalin) to the shrimp eggs and soak for 30 minutes, then ultrasonically clean them for 3 minutes, remove the floating matter on the surface of the liquid, rinse the shrimp eggs with distilled water for 5 times, and then wash the shrimp eggs with distilled water. Eggs were put into a graduated cylinder, and distilled water was added to set the volume to 50mL to obtain a shrimp egg dispersion (the total volume of the shrimp egg dispersion was denoted as V 0 );

[0031] (3) Counting: use a pipette gun to draw 5mL of the shrimp...

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PUM

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Abstract

The invention discloses a method for rapidly obtaining the brood amount of euphausia shrimps. The method comprises the following steps: (1) sampling and weighing; (2) dispersing and diluting to the specified volume; (3) counting; (4) calculating. After shrimp eggs are taken from the abdomen of a shrimp, the shrimp eggs are dispersed, cleaned and diluted to the spevified volume to obtain shrimp eggdispersion liquid, the shrimp egg dispersion liquid is directly counted by a zooplankton image scanning analysis system after being sampled, and finally the total egg carrying amount of the shrimp per unit mass is calculated, so that the operation is time-saving and labor-saving, and the measurement is rapid and convenient; and measurement efficiency and result accuracy are high.

Description

technical field [0001] The invention relates to a method for measuring the amount of eggs, in particular to a method for quickly obtaining the amount of eggs of real shrimps. Background technique [0002] In the current process of revitalizing the traditional marine fishery, shrimp is an important species in the marine fishery economy, and its reproductive performance is of great significance to the revitalization of the marine fishery economy. Most species of true shrimps (Caridea) among marine shrimps are benthic species. Although they have a certain swimming ability, they spend most of their time on the bottom surface or in the bottom, such as long-armed shrimps (Palaemonidae). ), Hippolytidae, Alpheidae, Crangonidae and Atyidae. [0003] At present, the amount of eggs held is usually used to reflect the fecundity of shrimp holding eggs, and whether the reproductive performance of shrimps has declined is monitored by continuously measuring the number of eggs held by shri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06K9/00B08B3/12
CPCB08B3/12G06V20/69
Inventor 李振华周永东朱文斌张亚洲
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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