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Lactobacillus fermentum PV22 with anti-virus capacity, and application of Lactobacillus fermentum PV22

A technology of Lactobacillus fermentum and PV22, applied in the field of microorganisms, can solve different problems and achieve the effects of reducing infectivity, convenient use and good anti-virus

Active Publication Date: 2021-03-19
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, numerous studies have demonstrated that different bacterial strains verified by genomic information to be of the same species may have completely different effects on the host

Method used

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  • Lactobacillus fermentum PV22 with anti-virus capacity, and application of Lactobacillus fermentum PV22
  • Lactobacillus fermentum PV22 with anti-virus capacity, and application of Lactobacillus fermentum PV22
  • Lactobacillus fermentum PV22 with anti-virus capacity, and application of Lactobacillus fermentum PV22

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Isolation and identification of embodiment 1 lactic acid bacteria

[0045] 1.1. Isolation of lactic acid bacteria

[0046] The hometown of longevity in the world - the feces of centenarians in Jiaoling County, Meizhou City, Guangdong Province, China were collected as samples. In a sterile environment, 0.1g of feces samples were added to 10ml of MRS liquid medium, shaken and mixed, and placed under anaerobic conditions at 37°C Under the enrichment culture for 24 hours, draw 0.5ml of the bacterial solution for gradient dilution. Add saline to make 10 -1 to 10 -8 Dilute the gradient bacterial suspension, choose 10 -6 、10 -7 、10 -8 Pipette 100 μl of the three gradient bacterial suspensions into the MRS agar medium, spread evenly with a spreader stick, and then culture at 37°C for 48 hours under anaerobic conditions. Pick the typical colony on the plate and put it on the MRS agar medium for streaking and purification. After purification, pick a single colony and inocul...

Embodiment 2

[0050] The cultivation of embodiment 2 lactic acid bacteria and the preparation of lactic acid bacteria fermentation liquid

[0051] 2.1. Culture of lactic acid bacteria

[0052] Inoculate Lactobacillus fermentum (L.fermentum) PV22 from a glycerol tube onto an MRS agar plate, culture it anaerobically at 37°C for 48 hours, observe the colony morphology, and observe the colony under a microscope, and find that the colony is white Round, its thallus is short rod-shaped.

[0053] A single colony of Lactobacillus fermentum (L.fermentum) PV22 was picked and inoculated into MRS broth medium, cultured anaerobically at 37°C for 48h, and a growth curve was made. It was found that the bacteria was cultured at 37°C for 12 hours to reach a stable period, and its fermentation method was heterozygous fermentation, which could produce acid and gas from glucose.

[0054] 2.2. Preparation of lactic acid bacteria fermentation broth

[0055] A single colony of Lactobacillus fermentum (L.fermen...

Embodiment 3

[0056] Evaluation of embodiment 3 lactic acid bacteria antiviral ability

[0057] 3.1. Cell culture and passage

[0058] The amplification and infection system of murine norovirus (MNV) was used.

[0059] Cell culture: mouse mononuclear macrophage RAW264.7 cells were cultured in DMEM medium containing 10% fetal bovine serum (FBS) at 37°C, 5% CO 2 . After 48 hours of culture, the cells can cover 80% of the bottom of the T25 cell culture flask, and the cells can be subcultured at this time.

[0060] Cell subculture: Use a sterile Pasteur pipette to remove the old medium in the bottle, and wash off the residual medium with PBS solution. Add 2ml of 0.25% EDTA-containing trypsin to the bottle, incubate at room temperature for 3 minutes, observe under the microscope that the cells withdraw synapses or the intercellular space increases, and immediately stop the digestion with serum-containing medium. Use a sterile Pasteur pipette to gently pipette the digested cells to detach and...

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Abstract

The invention discloses a lactobacillus fermentum PV22 with an anti-virus capacity, and application of the lactobacillus fermentum PV22. The Lactobacillus fermentum PV22 is collected in the GuangdongMicrobial Culture Collection Center (GDMCC) on December 18th, 2020, the collection address is 5th floor, No.59 building, No.100, Xianlie Middle Road, Guangzhou, Guangdong Province, and the collectionnumber is GDMCC No.61376. The lactobacillus fermentum PV22 can obviously lower virus infectivity, obvious virus-antagonizing efficacy can be achieved in short time, in addition, an anti-virus effect is increased along with prolonging of action time, and a post-infection survival rate of a virus infection cell can be improved. Therefore, the lactobacillus fermentum (L.fermentum) PV22 can be appliedto preparation of products (such as foods, medicines, health care products and the like) used for preventing or treating virus infection, and has a huge application prospect.

Description

Technical field: [0001] The invention belongs to the field of microorganism technology and the field of medicine technology, and in particular relates to Lactobacillus fermentum PV22 with antiviral function and application thereof. Background technique: [0002] Virus infection is one of the important diseases that threaten human health and cause human death all over the world. In the past 20 years, there have been three relatively large viral pneumonia outbreaks, especially the global outbreak caused by the novel coronavirus pneumonia (Corona Virus Disease 2019, COVID-19), which has seriously threatened human biological health and caused serious economic losses. . Therefore, finding effective and safe methods for virus prevention and control has great applied research value. [0003] Prevention and treatment of viral infections is a hot topic in global public health research. At present, the proven and effective means of virus prevention and control are mainly the develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/11C12Q1/689C12Q1/04A61K35/747A61P31/14A23L33/135C12R1/225
CPCC12Q1/689A61K35/747A61P31/14A23L33/135A23V2400/143
Inventor 李滢薛亮吴清平高珺珊商燕燕谢新强蒋同陈慧贞李龙岩蔡伟程程彤张菊梅
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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