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Method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea gigas triploid

A technology of Portuguese oyster and Pacific oyster is applied in the field of hybrid production of Portuguese oyster and Pacific oyster to produce oyster allotetraploid, which can solve the problems of low survival rate, inability to adapt to the high temperature climate in southern summer, and increase breeding risks, and achieves popularization and application. promising effect

Inactive Publication Date: 2021-03-23
世倍(厦门)海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Pacific oyster culture cycle is too long, it usually takes 2 to 3 years to be marketed, and the survival rate is low, which increases the risk of farming
As a low-temperature species, Pacific oysters cannot adapt to the high-temperature climate in southern summer, so they cannot be cultivated in the southern coastal areas of my country all year round.

Method used

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  • Method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea gigas triploid
  • Method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea gigas triploid

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (a) Selection and ripening of oysters: select 200 triploid third-year-old Pacific oysters (shell height 10-15 cm) with large individuals, good vigor, and no damage as reproductive females, and start from 2000 second-year-old Portuguese oysters. For ploidy (shell height 8-12cm), the individual with the top 10% of the total weight is taken as the breeding male parent. Then put the seed shells into the seed shell cultivation ponds and adopt the flowing water method to promote ripening. The biological bait is mainly artificially cultivated golden algae, chaetoceros and flat algae, and the algae water in the earthen pond is used as a backup bait source.

[0030] (b) Acquisition of triploid eggs: After the gonads of Pacific oyster species have matured, 50 triploid individuals of Pacific oyster were detected by a ploidy analyzer, and a total of 48 3N individuals were detected, and 20 were screened out through microscope observation Female 3N individuals with full gonads and we...

Embodiment 2

[0036] (a) Selection and ripening of oysters: select 200 triploid third-year-old Pacific oysters (shell height 10-15 cm) with large individuals, good vigor, and no damage as reproductive females, and start from 2000 second-year-old Portuguese oysters. For ploidy (shell height 8-12cm), the individual with the top 10% of the total weight is taken as the breeding male parent. Then put the seed shells into the seed shell cultivation ponds and adopt the flowing water method to promote ripening. The biological bait is mainly artificially cultivated golden algae, chaetoceros and flat algae, and the algae water in the earthen pond is used as a backup bait source.

[0037] (b) Acquisition of triploid eggs: After the gonads of Pacific oyster oysters had matured, 50 triploid individuals of Pacific oyster oyster were detected with a ploidy analyzer, and a total of 45 3N individuals were detected, and 16 were screened out through microscope observation. Female 3N individuals with full gona...

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Abstract

The invention discloses a method for producing allotetraploid by hybridizing crassostrea angulata diploid and crassostrea gigas triploid. According to the method, the crassostrea gigas triploid is used as a female parent, the full weight is used as a target, the crassostrea angulata diploid is preferably selected as a male parent, and the oyster allotetraploid is produced through drug-induced hybridization. The oyster allotetraploid obtained by the method has the eurythermic characteristic, and the oyster triploid produced by hybridizing the oyster allotetraploid with the common crassostrea angulata or crassostrea gigas diploid is suitable for coastal aquaculture of the south and north in China , and has wide popularization and application prospects.

Description

technical field [0001] The invention relates to a method for producing allotetraploid by shellfish hybridization, in particular to a method for hybrid production of oyster allotetraploid by Portuguese oyster and Pacific oyster. Background technique [0002] As an important marine organism, oysters are known as "milk in the sea" because of their delicious meat and high nutritional value. Oysters have a wide geographical distribution, fast growth, high yield, and high economic value. They are important mariculture objects in all countries in the world. [0003] The growth advantage of triploid oyster is obvious. Triploid oysters have 3 sets of chromosomes, so fecundity is extremely low, which means that on the one hand they can grow faster (their energy is used to grow rather than reproduce), and on the other hand they do not produce Or rarely produce gametes, which can ensure stable quality. Triploid oysters are formed by crossing tetraploid oysters with diploid oysters, s...

Claims

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Application Information

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IPC IPC(8): A01K61/54A01K61/17
CPCA01K61/17A01K61/54Y02A40/81
Inventor 朱李贤
Owner 世倍(厦门)海洋科技有限公司
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