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Marker screening identification and application of non-concentrated reduced apple juice based on non-targeted metabonomics

A technology of apple juice and markers, which is applied in the field of marker screening and identification of non-concentrated reduced apple juice, can solve the problems of lack of effective identification of non-concentrated reduced apple juice and concentrated reduced apple juice, etc., so as to avoid misjudgment and improve The effect of accuracy

Active Publication Date: 2021-03-26
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is currently a lack of markers and related technical means to effectively identify non-concentrated reduced apple juice and concentrated reduced apple juice

Method used

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  • Marker screening identification and application of non-concentrated reduced apple juice based on non-targeted metabonomics
  • Marker screening identification and application of non-concentrated reduced apple juice based on non-targeted metabonomics
  • Marker screening identification and application of non-concentrated reduced apple juice based on non-targeted metabonomics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] 1. Screening and verification of identification markers of non-concentrated reduced apple juice and concentrated reduced apple juice

[0079] 1. Sample collection and preparation

[0080] Non-concentrated reduced apple juice and concentrated apple juice samples were collected from typical areas such as Shaanxi and Shandong in my country. The sources included a factory in Shandong (recorded as group A) and three factories in Shaanxi (recorded as groups B, C and D). Comprehensive consideration Variety, place of origin, batch, sterilization method (pasteurization and ultra-high pressure non-thermal sterilization), etc.; each factory has no less than 18 samples of each kind of apple juice. NFC apple juice samples included pasteurized apple juice and ultra-autoclaved apple juice. Among them, the pasteurized NFC apple juice of group A was directly provided by the factory, and the pasteurized NFC apple juice of groups B, C and D were processed at 85°C for 15 seconds in the lab...

Embodiment 2

[0105] Based on the 21 kinds of marker molecules of Example 1, the PLS-DA model for constructing and distinguishing NFC apple juice and FC apple juice is established, which includes:

[0106] Get 199 parts of apple juice samples in Example 1 and centrifuge (26916g, 4 ℃) 15min in centrifuge, get supernatant afterwards and filter in the PES membrane filter tip of 0.45 and 0.22 μ m, then pack into sampling bottle, as Samples were used for subsequent analysis.

[0107] Data acquisition was performed using an ultra-high performance liquid chromatography tandem quadrupole time-of-flight high-resolution mass spectrometer. The specific parameters of the chromatographic system are as follows: All samples were analyzed using ExionLC ultra-high performance reversed-phase liquid chromatography system (SCIEX, Redwood City, CA, USA), mobile phase A was 0.2% formic acid aqueous solution, B was acetonitrile, and the flow rate was 300 μL / min; The injection volume was 2 μL; the chromatographic...

Embodiment 3

[0114] The PLS-DA model of Example 2 is used for actual identification of the samples to be tested.

[0115] Adopt the PLS-DA model of the identification NFC apple juice of embodiment 2 and FC apple juice to identify the apple juice sample to be identified, the method is as follows:

[0116] (1) Take the 71 apple juice samples not used in Example 2 as the apple juice samples to be identified, centrifuge in a centrifuge (26916g, 4°C) for 15min, then take the supernatant and put them in 0.45μm and 0.22μm PES Membrane filter head filter, then put into the sample bottle, as a sample for subsequent analysis.

[0117] Data acquisition was performed using an ultra-high performance liquid chromatography tandem quadrupole time-of-flight high-resolution mass spectrometer. The specific parameters of the chromatographic system are as follows: All samples were analyzed using ExionLC ultra-high performance reversed-phase liquid chromatography system (SCIEX, Redwood City, CA, USA), mobile p...

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Abstract

The invention discloses marker screening identification and application of non-concentrated reduced apple juice based on non-targeted metabonomics, and relates to the technical field of fruit juice identification. The invention discloses a marker for identifying non-concentrated reduced apple juice. The marker is selected from the following molecules: gallocatechin, catechin, taxifolin, p-hydroxybenzaldehyde, 5-methoxysalicylic acid, azelaic acid, caffeic acid, chlorogenic acid, epicatechin, eriodictyol, ferulic acid, isoquercetin, naringenin, fructosyl isoleucine, p-coumalaldehyde, p-coumaricacid, phloretin, phlorizin, procyanidine B1, quercetin-3-O-galactoside and rutin. The marker can be used for identifying non-concentrated reduced apple juice and concentrated reduced apple juice, andhas high accuracy.

Description

technical field [0001] The invention relates to the technical field of identification of concealed adulteration of fruit juices, in particular to marker screening and identification of non-concentrated reduced apple juice based on non-targeted metabolomics and its application. Background technique [0002] With the enhancement of people's consumption awareness and the improvement of living standards, people's pursuit of food has changed to pay more attention to the taste, nutrition and convenience of food. Therefore, improving the quality of processed food, effectively preventing changes in quality and safety during food processing, and meeting consumers' growing consumer demand for high-quality food are important issues in the field of food processing. [0003] Fruit juice has a broad market because of its good taste, rich in vitamins and other contents, and convenient drinking. Consumers are very concerned about the existence of natural ingredients, effective nutrients, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N27/62
CPCG01N30/02G01N30/72G01N27/62G01N33/025H01J49/26G01N30/34G01N30/86G01N30/88
Inventor 徐贞贞徐雷张继明冷传祝王雪王珂雯辛刚宋新飞
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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