A gene-directed knock-in vector construction method, system and platform

A construction method and gene technology, applied in the field of bioinformatics, can solve problems such as the inability to realize fixed-point knock-in of exogenous genes, complex and cumbersome operations, and low efficiency, and achieve the effects of flexible design, precise experimental operation, and improved output

Active Publication Date: 2021-11-02
广州赛业百沐生物科技有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned technical problems and defects that the traditional experimental operation cannot realize the fixed-point knock-in of exogenous genes, and the operation is complex and cumbersome, inefficient, time-consuming and laborious

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A gene-directed knock-in vector construction method, system and platform
  • A gene-directed knock-in vector construction method, system and platform
  • A gene-directed knock-in vector construction method, system and platform

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0076] To facilitate a better understanding of the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. Those skilled in the art can easily understand the present invention from the contents disclosed in this description. Other advantages and effects.

[0077] The present invention can also be implemented or applied through other different specific examples, and various modifications and changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention.

[0078] It should be noted that if there are directional indications (such as up, down, left, right, front, back...) in the embodiment of the present invention, the directional indications are only used to explain how to move in a certain posture (as shown in the accompanying drawings). If t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of biological information, and in particular relates to a method, system and platform for constructing a gene-directed knock-in carrier. The present invention obtains the original data information of the gene to be knocked in, and creates a skeleton corresponding to the gene to be knocked in, constructs a vector knock-in model; acquires the sequence data to be inserted in real time, and fixes the homology arm sequence of the gene to be knocked in, The exogenous gene and some functional elements are knocked into the carrier in an orderly manner according to the design rules, and the system and platform corresponding to the method can realize the targeted knock-in of the exogenous gene, making the genetic background simpler and the experimental operation more accurate and efficient. And it is simpler than traditional operation, more flexible in design, lower in cost, and shorter in cycle. In addition, the fixed-point knock-in of genes provides the possibility for future targeted gene therapy. Repairing functionally deficient DNA fragments into functional DNA fragments can achieve the purpose of gene therapy. At the same time, it can greatly increase output, liberate manpower, and improve work efficiency.

Description

technical field [0001] The invention belongs to the field of biological information, and in particular relates to a method, system and platform for constructing a gene-directed knock-in carrier. Background technique [0002] At present, there are two main means of studying gene function: one is the loss of gene function; the other is to achieve gene overexpression. Gene knock-in is an effective technique to achieve stable gene overexpression. Gene knock-in (Gene knock-in) refers to the gene editing technology that uses random integration, transposon system, homologous recombination, etc. to insert foreign functional genes into the genome, so that they can be expressed in cells. [0003] However, the current gene knock-in models include: conventional gene knock-in, point mutation, conditional point mutation, and humanization. Earlier gene knock-in methods were mainly integrated into the host genome through random integration or transposon systems, This knock-in method is hi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G16B20/50G16B30/00G06F30/20
CPCG16B20/50G16B30/00G06F30/20
Inventor 高翠白颖
Owner 广州赛业百沐生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products