Construction system of novel transposon mutant strain library

Abstract

The invention relates to a construction system of a novel transposon mutant strain library. The construction method comprises the following construction steps that (1) a novel transposon plasmid vector in which a transposase gene tnpA is additionally added in an ITRs region is constructed; (2) a transposase gene tnpA additionally added in the ITRs region can be regulated and controlled by an arabinose promoter gene araBAD and an arabinose profilin gene araC; (3) a transposon mutant strain of different species containing a transposon is obtained; (4) the transposon mutant strain has a transposon insertion sequence at any position of a genome; and (5) the transposon mutant strain library in various forms of insert genes in a whole genome range is randomly obtained. According to the construction system, the gram-negative bacterium transposon mutant strain library covering the whole genome can be systematically and efficiently obtained, which facilitates research on genome annotation and functional analysis.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows create an organism called Transposons that have been introduced onto other cells or plants without losing their ability to carry out specific functions like DNA replication. These modifications make it possible to modify certain aspects of these systems such as cell growth rates, resistance against antibiotic agents, etc., making them useful tools for researchers studying microorganisms' role in various fields including medicine (immunology) and biotechnologies (biomedicine).

Problems solved by technology

Technically speaking, there exists two types of molecular cloning systems: one uses enzyme activity from archaeon tissue or other sources for creating cells with specific functions like cell division, gene expression regulation, protein production, and drug discovery; another relies on random crossing between different segments called transposers during migration across generational boundaries without any changes being made at either end. These techniques allow researchersto study complex structures containing many thousands of proteins simultaneously while reducing complexity and improving data quality compared to previous methods.

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