The invention discloses a method for screening a regulation new gene participating in the biological process. A biologic informatics method is used for searching gene ontology for a tool, and the semantic relation in the specific biological process is screened out; in a genome annotation database of different species, gene set information in different species annotated to the semantic is screenedout; homology analysis is performed on the gene set information of the specific species, an orthologous gene in the species to be researched is screened out; comparison and analysis are performed on the gene and the reference gene participating in the biologic process in the species to be researched, and the regulation new gene participating in the biologic process in the specific species is screened out. On the basis that the specific biologic process has the advantage of high conservative property between different species, a method for screening the regulating new gene participating in thebiologic process is built, the supporting effect is supplied for further reconstructing a systematic gene regulation network, and the method is of a great significance in early disease diagnosis, individual treatment and medicine development.