Construction system of novel transposon mutant strain library, novel transposon mutant library and application

Abstract

The invention discloses a construction system of a novel transposon mutation library, plasmids can be transferred into a target strain through chemical conversion and electric conversion methods, then expression of transposase is induced, and the transposon mutation library almost covering the whole genome range can be obtained only through a mutant strain containing transposon. The construction system of the novel transposon library can not only increase the diversity of transposon mutation, but also facilitate the determination of the insertion position of a strain, and facilitate the research on genome annotation, functional analysis and drug resistance mechanism of pseudomonas aeruginosa.

Description

technical field [0001] The invention belongs to the technical field of gene mutation, in particular to a construction system of a novel transposon mutant strain library, a novel transposon mutation library and applications. Background technique [0002] Transposon (Tn), also known as jumping gene, refers to a group of genetic elements that can insert its own gene into any gene sequence in the genome, and is divided into compound transposon and complex transposon. The essence of resistance transposons is a jumping gene system that incorporates resistance genes within elements. The more famous transposon is Tn5, which can encode genes resistant to aminoglycoside antibiotics such as kanamycin and neomycin; Tn10 transposons are found in some Gram-negative bacteria, especially in Enterobacteriaceae Gene encoding resistance to tetracyclines. [0003] Insertion sequence (Insertion sequence, IS) is the simplest type of transfer element found in bacteria, with a general size of 600...

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[0007] Through the search, no patent publications related to the patent application of the present invention have been found

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