Application of mevalonic acid metabolic intermediate product in preparation of medicines for improving aging ovarian oocyte quality and meiosis process
A technology of mevalonate and oocytes, applied in the field of bioengineering, can solve problems such as unclear
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[0044]Example 1
[0045]This embodiment provides a medicament that improves the quality of aging ovarian oocytes and splitting processes, which contains 10 μmol / L leaf-free diaphragust (GGOH) in vitro maturation medium, and its group is 1 shown.
[0046]Table 1 Composition of components of in vitro mature medium containing GGOH
[0047] ingredient Volume / quality Final concentration BSA 0.12g3mg / ml Phemymycin mixture 400μL FSH 5μL 50IU / L LH 5μL 10IU / L Sodium pyruvate 100μL 0.23 mmol / L Glutamide 400μL 2 mmol / L M19939.082ml GgOH storage solution dissolved in anhydrous ethanol (50 mmol / L) 8μL 10μmol / L End volume 40ml
[0048]Application This in vitro maturation medium containing GgOH improves the quality and decay split process of aging ovarian oocytes, adding modes and experimental processes such asfigure 1 Down:
[0049](1) October C57BL / 6 fever intraperitoneal injection 10 Iu PMSG, and the ovaries were separated from the L15 medium, and the ovarium was transferred t...
Example Embodiment
[0059]Example 2
[0060]This embodiment provides a medicament that improves the quality of aging ovarian oocytes and the split process, which is shown in vitro maturation medium containing 20 μmol / L Fiiiol (FOH), and the group is shown in Table 2. .
[0061]Table 2 Composition of Components of in vitro mature medium containing FOH
[0062] ingredient Volume / quality Final concentration BSA 0.12g3mg / ml Phemymycin mixture 400μL FSH 5μL 50IU / L LH 5μL 10IU / L Sodium pyruvate 100μL 0.23 mmol / L Glutamide 400μL 2 mmol / L M19939.074ml FOH storage fluid dissolved in anhydrous ethanol (50 mmol / L) 16μL 20μmol / L End volume 40ml
[0063]Application of this FOH-containing in vitro mature medium to improve the quality and decay split process of aging ovarian oocytes:
[0064](1) October C57BL / 6 fever intraperitoneal injection 10 Iu PMSG, and the ovaries were separated from the L15 medium, and the ovarium was transferred to M2 medium, and the 1ml syringe was punctured by the sinus fol...
Example Embodiment
[0073]Example 3
[0074]This embodiment provides a medicament that improves the quality of aging ovarian oocytes and the split process, which is anhydrous ethanol injection preparation in which fragrant fragrant leaf alcohol (GgOH) is soluble, and the method of producing GgOH is dissolved. The 50 mmol / l GgOH reservoir was prepared in anhydrous ethanol, which was a GgOH anhydrous ethanol solution injection preparation.
[0075]Application of the GGOH anhydrous ethanol solution injection preparation improves the quality of aging ovarian oocytes and decreased split processes:
[0076](1) September decadency C57BL6 mice, divided into 2 groups (anhydrous ethanol solution) and GGOH (GgOH anhydrous ethanol solution injection preparation), administration daily, according to 40 mg / kg The body weight was taken with the control group and the GgOH group injection preparation, and the sterile physiological saline was diluted 10 times after intraperitoneal injection, 30 days of continuous treatment, s...
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