Application of OsBZR1 gene and encoded protein in regulation and control of rice sheath blight resistance
A technology of rice sheath blight and gene coding, applied in the application field of OsBZR1 gene and encoded protein in regulating rice sheath blight resistance, can solve the problems that the relationship has not been reported yet
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[0032] 1. Construction of mutants that suppress and overexpress OsBZR1 gene
[0033] The RNA of rice variety Nipponbare was extracted and reverse transcribed into cDNA. Using this cDNA as a template, using SEQ ID NO.3: 5'-GTCGACACTAGTCACACTCTTTGAAAAGCAATC-3' and SEQ ID NO.4: 5'-GGTACCGAGCTCCATCGCCTGCGATTATGCTAC-3' as primers to amplify to obtain a 347bp PCR product, the PCR product has SEQ The sequence shown in ID NO.1 is from nucleotide 981 to 1327 at the 5' end; the PCR product is a forward fragment.
[0034] Such as figure 1 As shown in middle A, the PCR product was digested with SpeI and SacI, and the resulting digested product was connected to the 14610bp pTCK303 vector backbone obtained by the same digestion, so that the PCR product was inserted forward into the SpeI and SacI sites of the pTCK303 vector , to get the intermediate carrier;
[0035] Then digest the PCR product with SalI and KpnI, and connect the resulting digested product to the 14612bp intermediate vect...
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