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A kind of effective part of Didancao and its application in the preparation of drugs with anti-respiratory syncytial virus effect

An effective part, syncytial virus technology, applied in the field of natural medicinal chemistry, can solve problems such as not fully understood, and achieve excellent anti-respiratory syncytial virus effect

Active Publication Date: 2022-04-19
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors believe that the basis of anti-RSV active substances against Echinocarpus is still not fully understood, and there are still a large number of effective parts and active ingredients with anti-viral effects in Echinocarpus to be further developed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of the effective part of embodiment 1 Glycyrrhizae chinensis

[0031] (1) Take 400g of the dry whole herb of Echinacea, heat and reflux extraction with 4L of ethanol with a volume fraction of 95%, the extraction time is 1.5h, and the number of extractions is 2 times, and the combined two extracts are concentrated and dried to obtain the obtained product. The ethanol extract of Echinopsis chinensis;

[0032] (2) Suspend the ethanol extract of Echinocodon chinensis with 10 times the amount of water, then extract with petroleum ether, ethyl acetate and n-butanol equivalent to water respectively, and concentrate the extract to obtain petroleum ether extract, ethyl acetate Ester extracts and n-butanol extracts;

[0033] (3) Put the sherwood oil extract on the silica gel column (the weight consumption of the silica gel filler in the silica gel column is 30 times that of the ethyl acetate extract), first use the sherwood oil and the acetic acid that the volume...

experiment example 1

[0054] Experimental Example 1 Study on the Anti-RSV Activity of the Effective Parts of Didancao

[0055] The anti-RSV activity of the effective parts of C. chinensis was tested by the plaque reduction test method, and the specific methods were as follows: (1) HEp-2 cells were inoculated in 24-well cell culture plates (1 mL per well, and the cell density was 2 × 10 5 cell / mL), then placed at 37°C, 5% CO 2 Culture in an incubator until a monolayer of cells grows; (2) add 100 μL of 100 PFU / mL respiratory syncytial virus suspension to each well, 100 μL of drugs with different concentrations, and set up a cell control group and a virus control group at the same time; place at 37°C, 5% CO 2 Incubate for 2 hours in the incubator. Every 15 minutes, gently shake crosswise to make the virus evenly and fully adsorbed and penetrate into the cells; (3) After the virus is adsorbed for 2 hours, discard the virus liquid, wash it twice with PBS, and add 0.5mL agarose gel covering solution to...

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PUM

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Abstract

The invention relates to the technical field of natural medicine chemistry, and specifically discloses an effective fraction of dandelion and its application in preparing medicines with anti-respiratory syncytial virus effects. The preparation method of the effective part of Echinacea, comprising the following steps: (1) extracting Echinacea ethanol to obtain Echinacea ethanol extract; (2) suspending the Echinacea ethanol extract in water, and then Use petroleum ether, ethyl acetate and n-butanol to extract petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (4) take the silica gel eluted part A and put it on the silica gel column again, use a mixed organic solvent B for elution, collect the eluted part, and obtain the described The effective part of Glycyrrhizae chinensis. The effective part of the chrysalis prepared by the method has excellent anti-respiratory syncytial virus effect.

Description

technical field [0001] The invention relates to the technical field of natural medicine chemistry, in particular to an effective fraction of Glycyrrhizae chinensis and its application in preparing medicines with anti-respiratory syncytial virus effects. Background technique [0002] Respiratory syncytial virus (Respiratory syncytial virus, RSV) infection is widespread in the world, the population is generally susceptible, and superinfection and co-infection with a variety of respiratory viruses can occur. Thus, RSV is a common human pathogen and a threat to the health of newborns worldwide. The infection routes of RSV are mainly divided into airborne transmission and contact transmission. It can enter the human body through the eyes, nose, and throat. Therefore, close contact with people or things carrying RSV will have a certain risk of infection, especially infants or those with low immunity. crowd. The clinical manifestations are mainly symptoms of upper respiratory tra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/08A61K36/28A61P31/14A61P11/00
CPCC07D493/08A61K36/28A61P31/14A61P11/00A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2236/55
Inventor 王国才李药兰李满妹张玉波陈能花吴忠南
Owner JINAN UNIVERSITY