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Elephantopus scaber effective part and application thereof in preparing medicine with respiratory syncytial virus resisting effect.

A technology of effective parts, Didan grass, applied in the field of natural medicinal chemistry, can solve problems such as incomplete understanding, and achieve the effect of excellent anti-respiratory co-packaged virus effect

Active Publication Date: 2021-05-07
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors believe that the basis of anti-RSV active substances against Echinocarpus is still not fully understood, and there are still a large number of effective parts and active ingredients with anti-viral effects in Echinocarpus to be further developed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Example 1 Preparation of the effective part of the gentle grass

[0031](1) Take the dried gender whole grass 400g, heat reflow extraction with a 4L volume fraction of 95%, the extraction time is 1.5 h, the number of extraction is 2 times, and the combined extract is concentrated and dried. Wyperlygrade ethanol extract;

[0032](2) The geodorrhyl ethanol extract is suspended with 10 times water, and then extracted with water equal amount of petroleum ether, ethyl acetate, and n-butanol, concentrated extracts from petroleum ether extract, acetic acid Ester extract and n-butyl alcohol extract;

[0033](3) The oil ether extract is 30 times the silica gel column (the silica gel filler in the silica gel column is 30 times the weight of ethyl acetate extract), and the volume ratio of 5 fold volume is 92: 8 petroleum ether and acetic acid. Hybrid organic solvent consisting of ethyl ester is eluted eluted elute; then eluting with a mixed organic solvent consisting of 85: 15% by 85:15, a mixed ...

experiment example 1

[0054]Study on Anti - RSV Activity of Effective Listening of Soil Test

[0055]The anti-RSV activity was tested by emperor deconsive experimental method, and the specific method was as follows: (1) Take Hep-2 cells in a 24-well cell culture plate (1 ml per hole, cell density is 2 × 10)5Cell / ml), then placed at 37 ° C, 5% CO2Cultured in the incubator to grow cell single layer; (2) 100 μl of 100 μL of 100 pFu / mL respiratory syncytial viral suspension, 100 μl of different concentrations, and a virus control group at 37 ° C, 5%. CO2Culture in the incubator for 2 h. Each 15min cross is gently shaken, and the virus is uniformly adsorbed and penetrated into the cell; (3) After the virus is adsorbed for 2 h, the virus is absorbed, washed twice with PBS, each hole added 0.5 ml agarose gel cover. After the liquid is solidified, 0.5 ml of the corresponding concentration of drug, the cell control group and the viral control group were added to the equal volume of the maintenance liquid; (4) pl...

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PUM

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Abstract

The invention relates to the technical field of natural medicinal chemistry, and particularly discloses an elephantopus scaber effective part and application thereof in preparing a medicine with a respiratory syncytial virus resisting effect. The preparation method of the elephantopus scaber effective part comprises the following steps: (1) extracting elephantopus scaber with ethanol to obtain an elephantopus scaber ethanol extract; (2) suspending the elephantopus scaber ethanol extract with water, and extracting with petroleum ether, ethyl acetate and n-butyl alcohol respectively to obtain a petroleum ether extract, an ethyl acetate extract and an n-butyl alcohol extract; (3) loading the petroleum ether extract on a silica gel column, eluting with a mixed organic solvent A, collecting an elution part, concentrating and drying to obtain a silica gel elution part A; and (4) loading the silica gel elution part A on the silica gel column again, eluting with a mixed organic solvent B, and collecting the elution part, thereby obtaining the elephantopus scaber effective part. The elephantopus scaber effective part prepared by the method has an excellent respiratory syncytial virus resisting effect.

Description

Technical field[0001]The present invention relates to the field of natural drug chemical techniques, and more particularly to a geodally effective site and its application in the preparation of a drug having anti-respiratory syphilosis.Background technique[0002]Respiratory Syncytial Virus (RSV) infection is widely existent worldwide, and the population is generally susceptible to repeated infections and common infections with a variety of respiratory viruses. Therefore, RSV is a common human pathogen, and there is a threat to the health of newborns in the world. The infection pathway of RSV is mainly divided into air dissemination and contact propagation, and can pass eye, nose, and larynge into the human body, so close contact with RSV, people or objects that have certain infection, especially infants or immunity. crowd. The clinical manifestations are mainly, high-fever, chest tightness, air promotion, wheezing, cough, etc., long-term infections can cause viruses to enter the lowe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/08A61K36/28A61P31/14A61P11/00
CPCC07D493/08A61K36/28A61P31/14A61P11/00A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2236/55
Inventor 王国才李药兰李满妹张玉波陈能花吴忠南
Owner JINAN UNIVERSITY