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Method for constructing zebrafish osteoporosis model

A construction method, osteoporosis technology, applied in the field of medicine, can solve the problems of high lethality, zebrafish embryo teratogenicity, zebrafish bone development defects, etc., and achieve the effect of simple operation

Inactive Publication Date: 2021-05-14
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, prednisolone or dexamethasone are the main drugs used in the construction of zebrafish osteoporosis models, but the above two drugs are glucocorticoid drugs, and it is very easy to use them due to inaccurate dosage when making models. Excessive immunosuppression leads to teratogenicity and high l

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  • Method for constructing zebrafish osteoporosis model
  • Method for constructing zebrafish osteoporosis model

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[0026]Example 1:

[0027]1. Construction method of lead-to-swim horses, osteoporosis model, including the following steps:

[0028](1) Configuring the mother liquor: Tested 5 g of lead acetate, PBAC powder dissolved in 50 ml double vapor, i.e., 100 mg / ml of acetate solution;

[0029](2) Configure the working fluid: Take 200 μl of the parent solution to add 800 μl of double vapor to the centrifuge tube to obtain a 20 mg / mlpbac solution; 900 μl of the parent solution is added to 1.5 ml of centrifuge tube to add 900 μl of double steamed water, 10 mg / ML PBAC solution; 50 μl of the parent solution was added to 1.5 ml of centrifuge tube to give 5 mg / ml PBAC solution;

[0030](3) Put the sexual mature zebra fish in the mating fish tank according to the proportion of female ratio = 1: 2, plug in the separator, and separate the heroges; overnight, take out the partition, and collect the fish egg after 2 hours;

[0031](4) Put the collected fish eggs into the fish water of the antibiotics, incubatio...

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Abstract

The invention discloses a method for constructing a zebrafish osteoporosis model. The method comprises the following steps: putting sexually mature zebrafish into a mating fish tank according to a female-male ratio of 1: (1.5-2.5), inserting a partition plate, and separating female zebrafish from the male zebrafish; taking out the partition plate after one night, collecting roes after 90-150 minutes, placing the roes in a culture solution containing bacteriostat, and performing constant-temperature incubating; after incubation, randomly dividing the roes into a blank control group and a lead acetate group, adding the culture solution containing bacteriostat into the blank control group, adding a lead acetate solution into the lead acetate group, and performing culture, wherein the final concentration of the lead acetate is 5-20 mg/L; and enabling the cultured zebrafish to be subjected to cartilage dyeing, and when the cultured zebrafish is compared with the blank control group, if the dyeing area of the lead acetate group is reduced, obtaining the zebrafish osteoporosis model. According to the method, the zebrafish osteoporosis model which is simple and convenient to operate is used, and the zebrafish osteoporosis model can be quickly established in a short time after treatment with the lead acetate solution. A simple, efficient and high-throughput biological model is provided for high-speed screening of medicines for preventing and treating osteoporosis and research of a medicine mechanism.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to a method for constructing a zebrafish osteoporosis model. Background technique [0002] Osteoporosis is a metabolic bone disease characterized by decreased bone mass, abnormal bone microstructure and increased risk of fracture. With the continuous increase of population aging in my country, the incidence of osteoporosis and the resulting fragility fractures are also increasing. At present, animal models of osteoporosis have been widely used in the study of the etiology, mechanism, prevention and treatment of osteoporosis. An ideal experimental animal model of osteoporosis should have the following characteristics: (1) Convenience: refers to the simple modeling method and convenient use of the model; (2) Relevance: refers to the model should be as close as possible to the human body conditions, disease process, The pathological changes and outcome should be as similar as possib...

Claims

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Application Information

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IPC IPC(8): A01K67/02A01K61/10
CPCA01K67/02A01K61/10A01K2267/03A01K2227/40Y02A40/81
Inventor 安艳丁洁严鋭杨乾磊魏园杰张晓云
Owner SUZHOU UNIV
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