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Gene-eluting coating material capable of repairing oxidative stress damage and preparation method thereof

A damage repair and coating material technology, applied in the field of biomedical engineering functional materials, to reduce oxidative stress and solve the problem of late thrombosis

Active Publication Date: 2021-10-12
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the field of cardiovascular stents, no attempt has been made to introduce Tom70 into recipient cells to reduce intracellular oxidative stress

Method used

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  • Gene-eluting coating material capable of repairing oxidative stress damage and preparation method thereof
  • Gene-eluting coating material capable of repairing oxidative stress damage and preparation method thereof
  • Gene-eluting coating material capable of repairing oxidative stress damage and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A method for preparing a gene-eluting coating material capable of repairing oxidative stress damage, comprising the following steps:

[0033] (1) Mix 1 mg / mL dopamine solution and 15 mg / mL polyethyleneimine solution to obtain a mixed solution, soak the cleaned iron alloy base material in the mixed solution, and copolymerize for 2 hours under the solution condition of pH 9 to obtain Amino-functionalized surfaces;

[0034] (2) the material obtained in step (1) is placed in a persulfate solution with a pH of 3, and the addition of a concentration of tannic acid and polyethyleneimine is a mixture of 0.5 mg / mL (the concentration of tannic acid is 0.2 mg / mL mL, the concentration of polyethyleneimine is 0.3mg / mL), the reaction temperature is controlled at 60°C, and after a further reaction of 50min, it is washed 3 times and then dried with nitrogen to obtain the target material A;

[0035] (3) Preparation of plasmid DNA that can enhance the expression of Tom70, the carrier is...

Embodiment 2

[0038] A method for preparing a gene-eluting coating material capable of repairing oxidative stress damage, comprising the following steps:

[0039] (1) Place the cleaned magnesium alloy base material in a low-temperature plasma generator, inject allylamine monomer under a vacuum of 0.5 Pa, and obtain the amino positively charged surface after glow discharge for 10 minutes;

[0040] (2) placing the material obtained in step (1) with a pH of 3 copper sulfate solution, adding a concentration of 0.5mg / mL of tannic acid and polyethyleneimine mixture (the concentration of tannic acid is 0.2mg / mL , the concentration of polyethyleneimine is 0.3mg / mL), the reaction temperature is controlled to be 60°C, after further reaction for 50min, after cleaning for 3 times, nitrogen drying is carried out to obtain the target material A;

[0041] (3) Preparation of plasmid DNA that can enhance the expression of Tom70, the carrier is a lentiviral vector, and the obtained gene carrier is the target...

Embodiment 3

[0044] A method for preparing a gene-eluting coating material capable of repairing oxidative stress damage, comprising the following steps:

[0045](1) Place the cleaned zinc alloy base material in a low-temperature plasma generator, inject allylamine monomer under a vacuum of 0.5 Pa, and obtain the amino positively charged surface after glow discharge for 10 minutes;

[0046] (2) placing the material obtained in step (1) with a pH of 3 in periodate solution, adding a mixture of tannic acid and polyethyleneimine with a concentration of 2 mg / mL (the concentration of tannic acid is 0.5 mg / mL mL, the concentration of polyethyleneimine is 1.5mg / mL), the reaction temperature is controlled at 60°C, after further reaction for 90min, after cleaning for 3 times, nitrogen drying is carried out to obtain the target material A;

[0047] (3) Preparation of plasmid DNA that can enhance the expression of Tom70, the carrier is a lentiviral vector, and the obtained gene carrier is the target m...

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Abstract

The invention provides a gene eluting coating material capable of repairing oxidative stress damage and a preparation method thereof, belonging to biomedical engineering functional material technology. The preparation method comprises: performing amino functionalization treatment on the surface of the cleaned metal base material. The above materials are placed in a solution rich in strong oxidants with a pH of 3 to 5, and a hydrophilic compound solution with a concentration of 0.1 to 5 mg / mL is added to obtain a micro-nano topological structure rich in reactive quinone groups and amino groups. The target material A. Prepare a lentiviral gene vector target material B capable of enhancing the expression of Tom70, wherein the vector is a lentiviral vector. The target material B is fixed on the surface of the target material A, that is, the target gene-eluting coating material with oxidative stress damage repair is obtained. The coating has a micro-nano topology, which can provide a protective breeding ground for gene carriers and create favorable conditions for gene delivery.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering functional materials, and in particular relates to a gene elution coating material capable of repairing oxidative stress damage and a preparation method thereof. Background technique [0002] Vascular stent implantation has become the main method for the treatment of coronary heart disease (CHD). However, early Bare Metal Stent (BMS) implantation can easily lead to excessive neointimal hyperplasia, resulting in stent The probability of restenosis (In-stent Restenosis, ISR) is relatively high (20%-30%); while drug-eluting stent (Drug-eluting Stent, DES) can be treated with anti-proliferative drugs (such as rapamycin, Evidence However, subsequent clinical reports found that while DES inhibited the excessive proliferation of vascular smooth muscle cells, it also inhibited the delayed healing of the endothelial cell layer, leading to the occurrence of late thrombus (Late StentThrombosi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/04A61L27/34A61L27/36A61L27/54
CPCA61L27/042A61L27/047A61L27/34A61L27/36A61L27/54A61L2300/258A61L2300/42A61L2300/62A61L2420/02
Inventor 余泓池刘肖珩向茂龙马佳罗日方曹成建沈阳
Owner SICHUAN UNIV