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Marker for endometrial cancer and detection kit thereof

A technology for endometrial cancer, detection kits, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc.

Pending Publication Date: 2021-05-25
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

lncRNA specifically related to endometrial cancer has not been reported

Method used

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  • Marker for endometrial cancer and detection kit thereof
  • Marker for endometrial cancer and detection kit thereof
  • Marker for endometrial cancer and detection kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Screening of the lncRNA system associated with the prognosis of endometrial cancer

[0040] Use bioinformatics analysis methods to find lncRNAs that are closely related to the occurrence and clinical prognosis of endometrial cancer, and discover and improve the lncRNAs system that can be used for the diagnosis and prognosis of endometrial cancer.

[0041]Extract the RNA expression profile and clinical information data (https: / / portal.gdc.cancer.gov / repository) of all endometrial cancer (UterineCorpus Endometrial Carcinoma, UCEC) in the (The Cancer Genome Atlas (TCGA)) database, including 548 UCEC tissues and 35 non-tumor tissues. LncRNAs are annotated by the human gene annotation file (GRCh38.p12), which has been downloaded from the Ensembl database (https: / / asia.ensembl.org / index.html). The expression data of lncRNAs were analyzed and intersected by R / Bioconductor software packages Limma, edgeR, and DESeq2. Differentially expressed lncRNAs were distinguishe...

Embodiment 2

[0050] Example 2 Detection of the expression level of 3-lncRNAs in tumor tissues of patients with endometrial cancer

[0051] The samples of endometrial cancer patients admitted to the Department of Obstetrics and Gynecology of the First Affiliated Hospital of Soochow University were collected, relevant clinical data were analyzed, and the real time RT-PCR method was used to detect lnc02086, lncFAM83D and lncARL6- 1, using GAPDH as an internal reference.

[0052] The primer sequences used to detect lnc02086, lncFAM83D, lncARL6-1, and GAPDH are as follows:

[0053] lnc02086-F: 5'-TTCCGGTGTCCAGATGTTCA-3'; SEQ ID NO.4;

[0054] lnc02086-R: 5'-AAGGTGAGTTGGGGAGGATG-3'; SEQ ID NO.5;

[0055] lncFAM83D-F: 5'-ATGAGTGTGGCAGCCTATGT-3'; SEQ ID NO.6;

[0056] lncFAM83D-R: 5'-AACTCCTGACCTCGTGATCC-3'; SEQ ID NO.7;

[0057] lncARL6-1-F: 5'-GGCCTTGAGAGATAGAGGGG-3'; SEQ ID NO.8;

[0058] lncARL6-1-R: 5'-ATACGTCCTTTCCCATCCTGC-3'; SEQ ID NO.9;

[0059] GAPDH-F: 5'-GCACAGTCAAGGCTGAGAATG-3';...

Embodiment 33-l

[0062] Correlation between the expression level of embodiment 33-lncRNAs and the clinical characteristics of endometrial cancer

[0063] In order to investigate the correlation between the expression level of 3-lncRNAs and the clinical features of endometrial cancer, according to the expression levels of lnc02086 (CTD_2377D24.6), lncFAM83D (RP4_616B8.5) and lncARL6-1 (RP11_389G6.3), the uterine Endometrial cancer patients were divided into 3-lncRNAs high expression level group and 3-lncRNAs low expression level group. The results of Pearsonchi-square and Fisher's Exact tests analysis showed that the expression level of 3-lncRNAs was related to the histological type of endometrial cancer, menopausal , clinical stage and tumor are closely related (Table 2), which provides a reliable research basis for future prospective research on the expression level of 3-lncRNAs and the clinical characteristics of endometrial cancer.

[0064] Table 2 Three sets of long non-coding RNAs are sig...

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Abstract

The invention discloses a marker for an endometrial cancer and a detection kit thereof, and belongs to the technical field of molecular detection. According to the marker for the endometrial cancer and the detection kit thereof disclosed by the invention, all endometrial cancer patient data in a TCGA database is utilized, differentially expressed lncRNAs are analyzed, and new lncRNAs capable of predicting the lifetime of endometrial cancer patients are searched by using multiple algorithms and clinical characteristics of related patients; and the marker for the endometrial cancer, namely lnc02086, lncFAM83D and lncARL6-1, is obtained, and the marker for the endometrial cancer is used for specific detection of endometrial cancer.

Description

technical field [0001] The invention relates to the technical field of molecular detection, in particular to a marker of endometrial cancer and a detection kit thereof. Background technique [0002] About 98% of the genes in the genome do not have protein-coding potential but are still actively transcribed, and these RNAs are called non-coding RNAs. Non-coding RNAs longer than 200 nucleotides are called long non-coding RNAs (lncRNAs). With the discovery of lncRNA's extensive biological effects in different tissue cells and the advancement of lncRNA research technology, scientists have also realized that lncRNA not only participates in the transcription, translation and modification of tumor cell regulation coding genes, but may also be a marker of tumorigenesis and a therapeutic target . [0003] In recent years, many studies have reported that lncRNA affects the gene expression of tumor cells, regulates transcription factors, translation, protein modification and other pr...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/178C12Q2600/158
Inventor 丁红梅李冰燕邓立峰王萍蒋菲畅磊曹志飞陈友国周金华王娟沈芳荣张红
Owner SUZHOU UNIV
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