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Gene cooperation as endometrial cancer marker and application thereof

A technology of endometrial cancer and biomarkers, applied in the determination/testing of microorganisms, biochemical equipment and methods, instruments, etc., can solve the problems of unpredictable recurrence, difficult screening of patients, and low sensitivity

Inactive Publication Date: 2021-11-26
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] At present, the most commonly used clinical detection markers are sugar chain antigen CA125 and human epididymis protein 4 (HE4). Among them, serum CA125 is helpful for monitoring clinical treatment effect for patients with extrauterine lesions, but for peritoneal inflammation or radiation injury CA125 may be abnormally elevated in patients with isolated vaginal metastases, but CA125 is not elevated in patients with isolated vaginal metastases, so recurrence cannot be predicted in the absence of other clinical findings. In addition, the sensitivity of CA125 and HE4 is low, and it is difficult to effectively screen patient

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  • Gene cooperation as endometrial cancer marker and application thereof
  • Gene cooperation as endometrial cancer marker and application thereof
  • Gene cooperation as endometrial cancer marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Screening of Biomarkers Related to Endometrial Cancer

[0073] 1. Screening method

[0074] (1) The data used for screening and preprocessing methods

[0075] In this example, the data for screening biomarkers related to endometrial cancer were downloaded from the GEO database and the TCGA database respectively, and the public gene expression data and complete clinical Note, among them, the microarray data and clinical information of the GSE17025 dataset were downloaded from the GEO database as a training set, and the sample size was endometrial cancer group: control group = 91:12; the RNA of endometrial cancer was downloaded from the TCGA database- The seq data and clinical information are used as the verification set, and the sample size is endometrial cancer group: control group = 543:35;

[0076] Use the double-end sequence automatic detection mode of fastp software to process the raw data; the minimum N base number threshold is 5, the reads minimum leng...

Embodiment 2

[0083] Example 2 Validation and analysis of the diagnostic efficacy of the screened biomarkers

[0084] 1. Verify the analysis method

[0085] The R package "pROC" was used to draw the receiver operating curve (ROC), and the biomarkers LDHA, RPS27A, and LDHA that were screened in Example 1 and showed significant differential expression between the control group and the endometrial cancer group were analyzed. The AUC value, sensitivity and specificity of +RPS27A were used to judge its diagnostic efficacy for endometrial cancer. Among them, when evaluating and analyzing the diagnostic efficacy of single biomarkers LDHA and RPS27A for endometrial cancer, the expression level of the gene (Log 2 expression level) for evaluation and analysis, select the point corresponding to the largest Youden index as its cutoff value, that is, the optimal division threshold is determined by the point with the largest Youden index; in the evaluation and analysis of the combined biomarker LDHA+RPS...

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Abstract

The invention discloses a gene cooperation as an endometrial cancer marker and application thereof. The gene combination is a combination of LDHA and RPS27A, and the gene combination has the advantages of high sensitivity, high accuracy and the like when being applied to diagnosis or early diagnosis of endometrial cancer. The gene combination provided by the invention provides an effective tool for diagnosis and treatment of endometrial cancer, and is beneficial to guidance of clinical treatment.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a gene combination as a marker of endometrial cancer and its application. More specifically, the gene combination described in the invention is LDHA and RPS27A. Background technique [0002] More than 90% of uterine cancers are endometrial cancers (Endometrial carcinoma, EC), originating from the epithelium, most of the remaining uterine cancers are mesenchymal carcinomas, originating from the myometrium, and a few are endometrial stromal carcinomas, which account for about 10% of women. 7% of all cancers and a woman's lifetime risk of developing endometrial cancer is 2.5%. EC is one of the three common malignant tumors of the female reproductive system, accounting for 20%-30% of malignant tumors of the female reproductive system. In recent years, the incidence of endometrial cancer has shown a significant upward trend worldwide. And gradually tends to be younger....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574
CPCC12Q1/6886G01N33/57442G01N33/57484C12Q2600/158
Inventor 杨承刚李雨晨刘乐凯
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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