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Application of products related to small molecular markers in disease diagnosis

A technology of biomarkers and products, applied in the field of biomedicine, can solve problems such as low sensitivity, difficulty in screening patients, and inability to predict recurrence.

Inactive Publication Date: 2021-10-22
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] At present, the most commonly used clinical detection markers are sugar chain antigen CA125 and human epididymis protein 4 (HE4). Among them, serum CA125 is helpful for monitoring clinical treatment effect for patients with extrauterine lesions, but for peritoneal inflammation or radiation injury CA125 may be abnormally elevated in patients with isolated vaginal metastases, but CA125 is not elevated in patients with isolated vaginal metastases, so recurrence cannot be predicted in the absence of other clinical findings. In addition, the sensitivity of CA125 and HE4 is low, and it is difficult to effectively screen patient

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  • Application of products related to small molecular markers in disease diagnosis
  • Application of products related to small molecular markers in disease diagnosis
  • Application of products related to small molecular markers in disease diagnosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Screening of Biomarkers Related to Endometrial Cancer

[0073] 1. Screening method

[0074] (1) The data used for screening and preprocessing methods

[0075] In this example, the data for screening biomarkers related to endometrial cancer were downloaded from the GEO database and the TCGA database respectively, and the public gene expression data and complete clinical Note, among them, the microarray data and clinical information of the GSE17025 dataset were downloaded from the GEO database as a training set, and the sample size was endometrial cancer group: control group = 91:12; the RNA of endometrial cancer was downloaded from the TCGA database- The seq data and clinical information are used as the verification set, and the sample size is endometrial cancer group: control group = 543:35;

[0076] Use the double-end sequence automatic detection mode of fastp software to process the raw data; the minimum N base number threshold is 5, the reads minimum leng...

Embodiment 2

[0083] Example 2 Validation and analysis of the diagnostic efficacy of the screened biomarkers

[0084] 1. Verify the analysis method

[0085] The R package "pROC" was used to draw the receiver operating curve (ROC), and the biomarkers MARCKSL1, RPS4X, and MARCKSL1 that were screened in Example 1 and showed significant differential expression between the control group and the endometrial cancer group were analyzed. The AUC value, sensitivity, and specificity of +RPS4X were used to judge its diagnostic efficacy for endometrial cancer. Among them, when evaluating and analyzing the diagnostic efficacy of single biomarkers MARCKSL1 and RPS4X for endometrial cancer, the gene expression (Log 2 expression level) for evaluation analysis, select the point corresponding to the largest Youden index as its cutoff value, that is, the optimal division threshold is determined by the point with the largest Youden index; in the evaluation and analysis of the combined biomarker MARCKSL1+RPS4X ...

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Abstract

The invention discloses application of products related to small molecular markers in diagnosis of diseases. The small molecular markers are MARCKSL1 and RPS4X, the products comprise reagents for detecting the small molecular markers, and the products can be used for auxiliary diagnosis of endometrial cancer, can be conveniently applied to clinical rapid and effective early diagnosis of endometrial cancer, and provide support for clinical doctors to timely adopt more personalized prevention and treatment schemes.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to the use of products related to small molecule markers in diagnosing diseases. More specifically, the present invention relates to the use of products related to small molecule markers in diagnosing endometrial cancer. Background technique [0002] More than 90% of uterine cancers are endometrial cancers (Endometrial carcinoma, EC), originating from the epithelium, most of the remaining uterine cancers are mesenchymal carcinomas, originating from the myometrium, and a few are endometrial stromal carcinomas, which account for about 10% of women. 7% of all cancers and a woman's lifetime risk of developing endometrial cancer is 2.5%. EC is one of the three common malignant tumors of the female reproductive system, accounting for 20%-30% of malignant tumors of the female reproductive system. In recent years, the incidence of endometrial cancer has shown a significant up...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574G01N33/68
CPCC12Q1/6886G01N33/57442G01N33/57488G01N33/57484G01N33/6893C12Q2600/118C12Q2600/158
Inventor 杨承刚李雨晨刘乐凯
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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