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Primer and kit for recognizing carcasses of silky fowl and black-bone silky fowl and application thereof

A kit and technology for silky chickens, which are applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the lack of effective methods for identifying silky silky chickens and black phoenix chicken carcasses, etc. question

Active Publication Date: 2021-05-25
NANCHANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no effective method to identify the carcasses of silky and black-bone chickens

Method used

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  • Primer and kit for recognizing carcasses of silky fowl and black-bone silky fowl and application thereof
  • Primer and kit for recognizing carcasses of silky fowl and black-bone silky fowl and application thereof
  • Primer and kit for recognizing carcasses of silky fowl and black-bone silky fowl and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]Example 1 Verify the difference between genome of silky horn bone chicken and black hanger

[0033]Step 1: Template preparation

[0034](1) Organizational Acquisition: Collect 6 silk feathers and 6 black chicken skin samples.

[0035](2) DNA template preparation: 50mg of left and right skin samples, adding 500 μl of liquid nitrogen centrifugal tube to ground the sampled sample to be collected. After the liquid is evaporated, 200 μl TE, 1200 rpm / min centrifuge is added, and the prepared is extracted.

[0036]Step 2: Purpose locus

[0037](1) PCR amplification

[0038]According to GenBank: DQ118701 and GenBank: DQ118702, three specific primers were designed to perform genotyping of the target site. The R primer is a common downstream of F and MF primers. F and R amplification obtained 542 bp strips, nucleotide sequences such as SEQ ID NO.4, MF and R primers amplified to obtain 310 bp strips, nucleotide sequences such as SEQ ID NO.5.

[0039]PCR amplification primers are shown in Table 1:

[0040]Tabl...

Embodiment 2

[0052]Example 2 Reliability of single-blind detection judgment method

[0053]In this embodiment, the sample of known varieties is used in a single blind manner, and the source of its variety is determined according to the molecular test results.

[0054]Step 1: Template Preparation

[0055](1) Organizational Acquisition: Collect 12 silky hooks and 12 black phoenix skin samples.

[0056](2) DNA template preparation: 50mg of left and right skin samples, adding 500 μl of liquid nitrogen centrifugal tube to ground the sampled sample to be collected. After the liquid is evaporated, 200 μl TE, 1200 rpm / min centrifuge is added, and the prepared is extracted.

[0057]Step 2: Purpose locus

[0058](1) PCR amplification

[0059]According to GenBank: DQ118701 and GenBank: DQ118702, three specific primers were designed to perform genotyping of the target site. The R primer is a common downstream of F and MF primers. F and R amplification obtained 542 bp strips, nucleotide sequences such as SEQ ID NO.4, MF and R ...

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Abstract

The invention discloses a primer and a kit for recognizing carcasses of silky fowl and black-bone silky fowl and the application thereof. The primer comprises an upstream primer F, a downstream primer F and a downstream primer F, wherein the nucleotide sequence of the upstream primer F is as shown in SEQ ID No.1; the nucleotide sequence of the upstream primer MF is as shown in SEQ ID No.2; the nucleotide sequence of the downstream primer R is as shown in SEQ ID No.3; according to the primer for recognizing the silky fowl chicken and the black-phoenix chicken, the genome DNA of the silky fowl chicken and the black-phoenix chicken with different lengths can be amplified, the silky fowl chicken and the black-phoenix chicken can be recognized very easily through the length of a gel electrophoresis band, the slaughtered silky fowl chicken and the slaughtered black-phoenix chicken can be accurately judged by 100%, and the method is simple and convenient to operate and high in practicability. The method can be used for recognizing or screening the silky-feather black-bone chicken and black-phoenix chicken carcasses in the iced fresh chicken market.

Description

Technical field[0001]The present invention relates to the field of molecular biological technology, and more particularly to primers, kits, and applications for identifying silky hypertens and black phenantoes.Background technique[0002]Sili Yuki Chicken is Jiangxi Thai and County Specialties, which is originally produced in the northern foothold of Thai County. According to the origin, it is called Wushan Chicken, because of "crown, sideware, green, beard, silk hair, feet, five Top ten characteristics and high nutritional value and medicinal value are known. Silk Yuki chicken is a famous dietary chicken with a whole body, bone, meat and internal organs have medicinal value, which can be equipped with a variety of pharmaceuticals and prescriptions.[0003]However, the original silky hypertrophy chickens, long-term slow, low egg production, and commercial breeding companies have cultivated black chicken with silky feathers and fast-growing varieties as breeding materials. Black and phoe...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124
Inventor 许继国马荆鄂周敏熊信威崔芳芳朱学农谭玉文许桥杨艳北贡继尚李袁飞盛文涛饶友生王樟凤
Owner NANCHANG NORMAL UNIV
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