Preparation and use method of a ratiometric fluorescent probe for visual discrimination detection

A ratiometric fluorescent probe and mass ratio technology, applied in chemical instruments and methods, fluorescence/phosphorescence, luminescent materials, etc., can solve problems such as difficult chemical and thermal stability, inability to distinguish tetracycline substances, and limited practical application range, etc. Achieve the effects of eliminating fluorescence interference, improving detection sensitivity, and mature preparation methods
CN112898965BActive Publication Date: 2022-04-19HENAN POLYTECHNIC UNIV
0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HENAN POLYTECHNIC UNIV
Publication Date
2022-04-19

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a method for preparing and using a ratiometric fluorescent probe for visual discrimination detection. The preparation method specifically includes the following steps: Step 1, preparation of silicon-based carbon quantum dots; Step 2, nano-mesoporous coating of silicon-based carbon quantum dots Preparation of molecularly imprinted materials; Step3, surface modification of nano-mesoporous molecularly imprinted materials; Step4, preparation of ratiometric fluorescent probes based on nano-mesoporous molecularly imprinted materials; the method of use includes: (1) Dilute tetracycline or oxytetracycline Solutions with different gradient concentrations; (2) The prepared ratio fluorescent probe based on nano-mesoporous molecularly imprinted materials was dispersed in ultrapure water, (3) Tris-HCl with pH=8 was added to the quartz cuvette After the buffer solution and the solution are mixed, the tetracycline solution or the oxytetracycline solution in the step (1) are respectively added dropwise for fluorescence measurement; the main matrix of the present invention is a silicon dioxide molecularly imprinted layer with a mesoporous structure, and the method is reliable and can mass-produced and widely used in society.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of preparing nanocomposite materials, in particular to a method for preparing and using a ratio fluorescent probe for visual discrimination and detection. Background technique

[0002] Both tetracycline and oxytetracycline are the most widely used antibiotics in the tetracycline class, commonly used to treat human and animal infections caused by Gram-positive and Gram-negative bacteria. Due to their significant antibacterial effects, tetracycline antibiotics are often used as feed additives, used to prevent and treat diseases, and promote animal growth and development during animal feeding. However, the irrational use of tetracycline antibiotics, including tetracycline (TC) and oxytetracycline (OTC), may lead to the accumulation of tetracycline antibiotics. Although antibiotics have helped humans defeat many diseases caused by bacteria, more than 10% of the human population also suffers from side effects of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More