Preparation and use method of a ratiometric fluorescent probe for visual discrimination detection

Abstract

The invention discloses a method for preparing and using a ratiometric fluorescent probe for visual discrimination detection. The preparation method specifically includes the following steps: Step 1, preparation of silicon-based carbon quantum dots; Step 2, nano-mesoporous coating of silicon-based carbon quantum dots Preparation of molecularly imprinted materials; Step3, surface modification of nano-mesoporous molecularly imprinted materials; Step4, preparation of ratiometric fluorescent probes based on nano-mesoporous molecularly imprinted materials; the method of use includes: (1) Dilute tetracycline or oxytetracycline Solutions with different gradient concentrations; (2) The prepared ratio fluorescent probe based on nano-mesoporous molecularly imprinted materials was dispersed in ultrapure water, (3) Tris-HCl with pH=8 was added to the quartz cuvette After the buffer solution and the solution are mixed, the tetracycline solution or the oxytetracycline solution in the step (1) are respectively added dropwise for fluorescence measurement; the main matrix of the present invention is a silicon dioxide molecularly imprinted layer with a mesoporous structure, and the method is reliable and can mass-produced and widely used in society.

Description

technical field [0001] The invention relates to the technical field of preparing nanocomposite materials, in particular to a method for preparing and using a ratio fluorescent probe for visual discrimination and detection. Background technique [0002] Both tetracycline and oxytetracycline are the most widely used antibiotics in the tetracycline class, commonly used to treat human and animal infections caused by Gram-positive and Gram-negative bacteria. Due to their significant antibacterial effects, tetracycline antibiotics are often used as feed additives, used to prevent and treat diseases, and promote animal growth and development during animal feeding. However, the irrational use of tetracycline antibiotics, including tetracycline (TC) and oxytetracycline (OTC), may lead to the accumulation of tetracycline antibiotics. Although antibiotics have helped humans defeat many diseases caused by bacteria, more than 10% of the human population also suffers from side effects of...

AI Technical Summary

Problems solved by technology

At the same time, the detection of TC / OTC by a single europium ion will be interfered by other organic substances, and the specificity is poor; most importantly, it is impossible to distinguish the same class of tetracycline substances with similar structures, and its chemical and thermal stability have always been difficult to solve problems; secondly, products are often not mass-produced, which limits their practical application range

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