Rapid determination method for crop pollination intensity

A crop and plant technology, applied in the field of crop pollination effect evaluation, can solve the problems of fixing or softening for a long time, and the softening time cannot be accurately grasped, so as to shorten the time required for fixing and softening, shorten the time required for softening, and is easy to operate. Effect

Active Publication Date: 2021-06-04
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented describes an improved way to improve the efficiency with which we plant crops during spring growth periods. By applying certain chemicals called calcium hydreate (CaH2) onto specific parts of plants' leaves before harvesting them later, this process helps prevent damage from bacteria that may harm their roots when they break off. Additionally, there has been previous research showing how these treatments could be applied more effectively than other methods like pesticides. Overall, this new approach allows us to enhance our ability to control insect infestations without causing negative side consequences such as increased labor expenses associated with traditional techniques.

Problems solved by technology

This patents describes methods for assessing how well plants respond to environmental stress factors like water availability. These techniques involve studying changes over time caused by external influences such as temperature fluctuate, light levels, nutrient supply rates, soil moisture content, pests' numbers, etc., while also considering different types of polypers with specific characteristics. By comparing these measurements against each other, researcher may determine if there was sufficient polytelling ability at all stages before harvesting. Additionally, this technique allows scientists to quickly observe and measure the strength of their response towards various stresses without having to wait for days until they become too weak again.

Method used

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  • Rapid determination method for crop pollination intensity
  • Rapid determination method for crop pollination intensity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 blueberry flower sample marking and collection

[0048]At around 10 am, the sampling points were selected in the blueberry field with the five-point sampling method, and the number of marked samples at each sampling point was not less than 20. At the sampling point, select flowers with a corolla opening diameter of 1-2mm to hang a hanging tag for marking, and record the marking time and sample number in detail on the hanging tag (such as figure 1 shown).

[0049] At about 10:00 am the next day after marking, the flowers were marked the day before the inspection. The diameter of the corolla opening should not be less than 5mm, and the flowers are considered to be open. Indicate the opening date of the flower on the tag of the flower that has bloomed.

[0050] At about 10:00 am the next day after the flowers bloom, use ophthalmic scissors to remove the corolla, stamens and other structures in the field, and cut off the stigma with a length of about 5mm at t...

Embodiment 2

[0051] Example 2 Fluorescence Microscopic Observation of Blueberry Pollen Tubes

[0052] Put the stigma collected by Example 1 into a 1.5mL centrifuge tube, use the fixative provided by the present invention (formaldehyde accounts for 3-4%, ethanol accounts for 50-70%, acetic acid accounts for 10-20%, and the rest water) for fixation, and fix at room temperature for 1 hour.

[0053] After the fixation, the fixative was discarded, and the head of the column was washed 3 times with distilled water. After discarding the distilled water, add preheated 4mol / L sodium hydroxide solution softening solution to soak the head of the column, and soften it in a water bath at 70°C for 1 hour.

[0054] After softening, the softening solution was discarded, and the head of the column was washed 3 times with distilled water. After discarding the distilled water, add 0.1% aniline blue aqueous solution to soak the column head for staining, and stain at room temperature for 1 hour.

[0055] Af...

Embodiment 3

[0057] Embodiment 3 blueberry pollination intensity evaluation method

[0058] The image observed in embodiment 2 is analyzed, at first according to image judgment has the proportion of the stigma of pollen tube germination, calculation formula is as follows:

[0059] Percentage of stigmas with pollen tube germination (%)=(number of stigmas with pollen tube germination / total number of stigmas collected)*100%;

[0060] Secondly, use image analysis software to identify the pollen tubes and count the area of ​​the stigma occupied by the pollen tubes and the total area of ​​the stigma, so as to calculate the proportion of the pollen tubes to the stigma area. The calculation formula is as follows:

[0061] Percentage of pollen tube area in stigma (%)=(area of ​​pollen tube / total area of ​​stigma)*100%.

[0062] In the method for quickly measuring the pollination intensity of crops in the present invention, the crops include but not limited to grains, melons and fruits, vegetables,...

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Abstract

The invention relates to a rapid determination method for crop pollination intensity, which comprises the following steps: (1) labeling flower buds about to bloom in the next day in a field, and collecting and labeling pistil stigmas of the flowers 24-72 hours after the flowers bloom; (2) fixing the pistil stigma by using a stationary liquid, softening by using a sodium hydroxide solution and dyeing by using an aniline blue aqueous solution in sequence to prepare a slide sample, and photographing and recording under ultraviolet irradiation; and (3) counting the proportion of the positive stigma with pollen tube germination, and counting the proportion of the pollen tube to the stigma area by image analysis software to indicate the pollination intensity. According to the method, the pollination intensity of the crops can be detected timely, quickly and visually, the destructiveness is small, and the accuracy is high.

Description

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Claims

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Application Information

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Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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