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Method for inducing spontaneous cataract by medicine (direction-regulating inducer MNU)

An inducer, spontaneous technology, applied in the field of cataracts, can solve problems such as blurred signs of photoreceptor activity, and achieve the effect of reducing the level

Inactive Publication Date: 2021-06-08
PEOPLES HOSPITAL OF HENAN PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Eventually at seven days, signs of activity from photoreceptor degeneration were obscured by loss of photoreceptors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Effects of melatonin on photoreceptor survival

[0070]OCT detection showed that the retinal structure of the MNU group was severely damaged. The retinal thickness in the MNU+vehicle group was significantly different from that in the MNU group, suggesting that intravitreal injection surgery cannot affect the outcome of MNU-induced retinal degeneration. Compared with the MUN group, the retinal structure in the MNU+melatonin group was relatively intact. And the retinal thickness of MNU+melatonin group was significantly thicker than that of MNU group. The outer nuclear layer (ONL) was not detected in the tissue sections of the MNU group, whereas several layers of the nucleus were preserved in the ONL in the MNU+melatonin group. The average thickness of the outer nuclear layer in the MNU+melatonin group was thicker than that in the MNU group. A large number of TUNEL-positive cells were found in the outer nuclear layer of the MNU group. In contrast, few TUNEL-positive ce...

Embodiment 2

[0072] Protective effect of melatonin on visual function

[0073] Typical ERG responses were produced in normal controls. According to previous studies retinal dot maps were not detectable in the MNU group. The amplitude of b wave in MNU+melatonin group was significantly larger than that in MNU group. The b-wave amplitudes of dark vision and bright vision were 56.1% and 62.7% of those of the normal control group, respectively. These data suggest that melatonin has a significant protective effect on visual function in MNU-administered mice. Dose-response analysis showed that the b-wave amplitude was greater at the 150 μg / kg dose than at the 50 μg / kg and 100 μg / kg doses. The amplitude of the b wave at the 150 μg / kg dose was not significantly different from the 200 μg / kg and 250 μg / kg doses. In the optokinetic test, the mice in the MNU group were not sensitive to the stimulus of the grating. The visual sensitivity in the MNU+vehicle group was exactly the same as that in the ...

Embodiment 3

[0075] Protective effects of melatonin on cone photoreceptor survival

[0076] Intense peanut lectin fluorescence was found in the inner section of the normal control group. Peanut lectin fluorescence was very unclear in the retinal sections of the MNU group. In contrast, peanut lectin fluorescence was found within the MNU+melatonin group. Whole retinal sections in the MNU+melatonin group showed that peanut agglutinin fluorescence was well preserved. The average number of peanut agglutinin-positive cells in the MNU+melatonin group was 723±58, and that in the MNU group was 15±10. These results indicate that melatonin treatment can significantly improve the survival of cone photoreceptors. In whole retinal sections of the MNU+melatonin group, the number of peanut agglutinin-positive cells was highest in the DT quadrant, indicating that cone photoreceptors were rescued by melatonin in this region. However, M-opsin or S-opsin staining could not be detected in the retinal secti...

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Abstract

The invention discloses a method for inducing spontaneous cataract by a medicine (a direction-regulating inducer MNU). The method comprises the following inducing steps: a, firstly, selecting 280 mice for research; b, after the step a is completed, placing the mice in free equipment of special pathogens, where water and foods are free to the mice; c, after the step b is completed, randomly dividing the mice into a common control group, an MNU group, an MNU+ melatonin group and an MNU+ blank group; and d, after the step c is completed, performing visual movement behavior test on the mice, determining a reaction threshold value through a function of correct tracking reaction, setting initial stimulation as a sine mode of 0.200 weeks / degree and fixed 100% comparison, and then performing electroretinogram examination on the mice. According to the invention, the melatonin is added into the inducer MNU, so that free radicals can be directly removed, the generation of endogenous antioxidant enzymes can be improved, the melatonin treatment is beneficial to the generation of Cu-Zn-SOD and MnSOD, and the levels of lipid and DNA peroxidation markers MDA and 8-OHdG are reduced.

Description

technical field [0001] The invention relates to the technical field of cataracts, in particular to a method for inducing spontaneous cataracts with drugs (modulation inducer MNU). Background technique [0002] The retina is the light-sensitive tissue located at the posterior pole of the eye. It arises from a layered structure of complex microcircuits that work together to process visual signals. Damage to the retina will cause irreversible visual damage. Retinitis pigmentosa is a group of inherited retinal diseases characterized by degenerative photoreceptor death. The pathological process of RP is influenced by molecular, cellular and tissue level factors. Given the enormous heterogeneity affecting etiology, RP patients invariably have greatly variable onset, progressive dynamics and their prognostic outcomes. These variability pose great challenges to precision therapy. At the clinical stage, no drug can cause photoreceptor death and interruption of visual impairment ...

Claims

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Application Information

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IPC IPC(8): A01K67/02A61K31/4045A61K49/00A61P27/02A61P39/06
CPCA01K67/02A61K31/4045A61K49/0008A61P27/02A61P39/06
Inventor 陶冶杜恩明王刚宋宗明
Owner PEOPLES HOSPITAL OF HENAN PROV
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