Liquid bio-organic fertilizer for preventing root rot as well as preparation method and application thereof
A bio-organic fertilizer and liquid technology, applied in the field of agricultural soil bio-fertilizer, can solve the problems of aging, enhanced chemical pesticide resistance, reduced chemical pesticide effect, etc.
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Embodiment 1
[0024] Acquisition of bacterial fluid
[0025] After isolation and purification from the soil, Lactobacillus crispatus and Bacillus cohesin were obtained by screening. After activation, the activated colonies were picked respectively, inserted into 1L LB culture medium, placed in a constant temperature shaking incubator at 30°C, and cultivated at 200rpm for 24 hours. bacteria liquid.
[0026] Place the obtained bacterial liquid in the fermenter and cultivate it in the PDA culture medium, so that the target bacterial strain in the fermenter reaches 1×10 8 ~1×10 9 cfu / ml.
[0027] The two cultured strains were mixed according to the colony forming unit number ratio of 1: (0.8~1) to obtain a composite bacterial liquid.
Embodiment 2
[0029] 1. Preparation of Liquid Bioorganic Fertilizer 2
[0030] (1) Preparation of decomposed compost products
[0031] Using 60 parts of pig manure, 30 parts of medicinal dregs and 25 parts of fruit peel as raw materials, the decomposed compost product was obtained by composting and fermenting for 7 days after inoculating yeast.
[0032] The medicinal residues are a mixture of medicinal residues from Dacha root and yellow sunflower, and the mass ratio is 1:1.
[0033] The peel is banana peel.
[0034] The yeast inoculum was 1×10 5 cfu / g.
[0035] (2) Mix the decomposed compost product with water, the mixing ratio is 1:12, and filter through a 100-mesh sieve to obtain the decomposed compost filtrate;
[0036] (3) Mix the decomposed compost filtrate with the molasses fermentation liquid, and inoculate the microbial complex bacterial liquid, and the inoculum amount of the complex bacterial liquid is 1×10 5 cfu / ml;
[0037] The solid content of the molasses fermentation li...
Embodiment 3
[0046] 1. Preparation of Liquid Bioorganic Fertilizer 3
[0047] (1) Preparation of decomposed compost products
[0048] Using 50 parts of cow manure, 40 parts of medicinal dregs and 20 parts of fruit peel as raw materials, the decomposed compost product was obtained by composting and fermenting for 5 days after inoculating yeast.
[0049] The medicinal residues are a mixture of medicinal residues from Dacha root and yellow sunflower, and the mass ratio is 1:1.2.
[0050] The peel is orange peel and grapefruit peel.
[0051] The yeast inoculum was 1×10 5 cfu / g.
[0052] (2) Mix the decomposed compost product with water at a mixing ratio of 1:15, and filter through a 100-mesh sieve to obtain the decomposed compost filtrate;
[0053] (3) Mix the decomposed compost filtrate with the molasses fermentation liquid, and inoculate the microbial complex bacterial liquid, and the inoculum amount of the complex bacterial liquid is 1×10 5 cfu / ml;
[0054] The solid content of the mo...
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