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A kind of yellow big tea acidic polysaccharide with lipid-lowering activity and its preparation method and application

A technology for acid polysaccharide and large tea, which is applied in the field of yellow tea acid polysaccharide and its preparation, can solve the problems of unclear material basis of active ingredients, and achieve the effect of significant lipid-lowering function, broad application prospect and reducing lipid accumulation.

Active Publication Date: 2022-04-29
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, tea polysaccharides, as the main active ingredient of tea, have been systematically researched and developed in teas such as Pu'er tea (black tea), green tea, and oolong tea. However, the material basis of its active ingredients is still unclear, and the main active ingredients of tea polysaccharides in yellow tea and their physical and chemical properties and efficacy relationship need to be clarified urgently

Method used

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  • A kind of yellow big tea acidic polysaccharide with lipid-lowering activity and its preparation method and application
  • A kind of yellow big tea acidic polysaccharide with lipid-lowering activity and its preparation method and application
  • A kind of yellow big tea acidic polysaccharide with lipid-lowering activity and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Preparation of yellow tea acidic polysaccharide LYTP-2

[0036] 1. Pulverization: crush the yellow tea and pass through an 80-mesh sieve to obtain the yellow tea powder;

[0037] 2. Degreasing: add 1000g of the yellow tea powder obtained in step 1 to 95% ethanol according to the liquid-to-solid ratio (g / mL) of 1:10, extract at 70°C for 2-3 hours, repeat the extraction for 2-3 times, and collect the tea residue after filtering , to dry;

[0038] 3. Extraction: add the tea filter residue obtained in step 2 into pure water at a liquid-to-solid ratio (g / mL) of 1:20, extract at 90°C for 2-3 hours, repeat the extraction for 3-4 times, and combine the extracts;

[0039] 4. Alcohol precipitation: Concentrate the extract obtained in step 3 to 1000mL, add 4000mL of 95% ethanol, let stand at 4°C for 10-12h, centrifuge at 5000rpm for 10-15min, and collect the precipitate;

[0040] 5. Deproteinization / decolorization of polyamide chromatography: using AKTApurifier TM 10...

Embodiment 2

[0042] Example 2: Determination of neutral sugar, uronic acid and monosaccharide composition of yellow tea acidic polysaccharide LYTP-2

[0043] 1. Determination of neutral sugar content of acidic polysaccharide LYTP-2 in yellow tea by phenol-sulfuric acid method. Accurately draw 2mL LYTP-2 solution (50μg / mL) and glucose standard solution, add 1mL 6% phenol and 5mL concentrated sulfuric acid, set up three parallel groups, measure the absorbance at 490nm, the measured neutral sugar content of LYTP-2 is 23% .

[0044] 2. Determination of the acidic uronic acid content of yellow tea acidic polysaccharide LYTP-2 by carbazole-sulfuric acid method. Accurately draw 1mL LYTP-2 solution (50μg / mL) and galacturonic acid standard solution, add 5mL 0.478% sodium tetraborate-sulfuric acid in ice water bath, boil water bath for 20min, add 0.2mL 0.15% carbazole after cooling, mix and let stand 2h, set up three groups in parallel, measure the absorbance value at 520nm, the measured uronic ac...

Embodiment 3

[0047] Example 3: Determination of lipid-lowering activity of polysaccharides from yellow tea

[0048] The lipid-lowering activity of LYTP-1, LYTP-2, LYTP-3 and LYTP-4, polysaccharide components of yellow tea polysaccharides, was evaluated by using oleic acid-induced HepG2 cells and normal liver cells LO2 as models.

[0049] 1. Detection of lipid-lowering activity

[0050] (1) Detection of total lipid: take HepG2 cells in good growth state, inoculate in 48-well plate, place in 5% CO 2 After 24 hours of adaptive culture in the incubator, remove the medium, add fresh medium to the blank control group, add 0.2mM oleic acid solution to the model group, add 0.2mM oleic acid and 400μg / mL LYTP-1, LYTP-2 to the experimental sample group , LYTP-3, and LYTP-4 solutions, continue to culture for 24 hours, stain with Oil Red O, observe and take pictures under a microscope (see Figure 4 ). After taking pictures, add 200 μL of isopropanol to each well to measure the total lipid content, ...

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Abstract

The invention discloses a yellow tea acidic polysaccharide with lipid-lowering activity and its preparation method and application, wherein the molecular weight of yellow tea acidic polysaccharide component LYTP‑2 is 4.57×10 4 Da, uronic acid content is more than 62%, and its monosaccharide composition and molar ratio are galacturonic acid: glucuronic acid: arabinose: galactose: rhamnose: glucose: mannose=69.45:1:10.41:10.13 :9.78:6.03:1.36. The acidic polysaccharide component LYTP-2 of yellow tea of ​​the present invention is obtained by combining polyamide chromatographic decolorization and ultrafiltration membrane separation polysaccharide component technology, has a significant lipid-lowering function, and can significantly reduce the lipid of HepG2 cells induced by oleic acid Accumulation, and no damage to normal liver cells LO2. LYTP‑2, as a new type of natural high-activity lipid-lowering polysaccharide, can be widely used in the fields of health food and medicine and health.

Description

technical field [0001] The invention belongs to the field of health food, and in particular relates to a yellow tea acidic polysaccharide with lipid-lowering activity and its preparation method and application. Background technique [0002] As one of the most important non-alcoholic beverages in the world, tea is loved by more and more consumers because of its unique aroma and taste. In recent years, the health benefits of drinking tea have become a topic of intense concern in food science and nutrition. According to the degree of fermentation, tea can be divided into green tea (unfermented tea), white tea (slightly fermented), yellow tea (partially fermented), oolong tea (semi-fermented tea), black tea (fully fermented tea), black tea (fermented tea) tea) and other six types. Scientific research shows that tea polyphenols, tea polysaccharides, alkaloids, theanine and other active ingredients in tea have various effects such as anti-oxidation, anti-tumor, enhancing immunit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23L33/125A61K31/715A61P3/06
CPCC08B37/006C08B37/0003A23L33/125A61P3/06A61K31/715A23V2002/00A23V2200/3262A23V2250/51
Inventor 陈彦严晨晨陈浩张坤峰黄宇哲周灿灿张婵吴前震郭文强
Owner ANHUI UNIVERSITY
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