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A Strain of Goose Astrovirus and Its Application

A technology of astrovirus and gosling, applied in the production of vaccines, goose astrovirus and its application field, can solve the problems of little research on goose astrovirus vaccine, no commercialization, large economic loss, etc., and achieve virus prevention Infection, strong virulence, and good immunity

Active Publication Date: 2022-06-28
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lack of suitable proliferative cell lines in vitro has resulted in relatively few attenuated or inactivated vaccines that can prevent and control avian Astrovirus infection, and there are no commercial goose Astrovirus vaccine products
[0004] The research and development of a variety of vaccines have been reported in the prior art. For example, Rong Xuelu et al. have developed and verified the immune effect of the recombinant Capsid protein subunit vaccine of goose Astrovirus. The research results show that the immune effect of the recombinant Capsid protein subunit vaccine of goose Astrovirus is Good, can effectively prevent the infection of goose astrovirus; patent document CN110699331A reports a preparation method and application of a goose astrovirus attenuated virus, which adopts a method of combining high temperature treatment and low temperature incubation to attenuate the virus for the preparation of vaccines It can effectively prevent goose gout caused by goose astrovirus. However, there are still few researches on goose astrovirus vaccines. Based on the current spread of goose astrovirus in farms, the economic losses caused are increasing. Therefore, There is an urgent need to develop safe and effective vaccines, yolk antibodies and drugs for the prevention and treatment of goose Astrovirus, in order to effectively control the Goose Astrovirus epidemic and avoid economic losses in the breeding industry

Method used

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  • A Strain of Goose Astrovirus and Its Application
  • A Strain of Goose Astrovirus and Its Application
  • A Strain of Goose Astrovirus and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation and identification of goose astrovirus

[0023] 1. Collection of disease materials

[0024] The diseased material samples were collected from the diseased farm in Nanyang, Henan Province. The diseased goose species included: Taizhou goose and Langde goose. The dead goslings showed joint swelling and white free urate deposition. The necropsy showed that white urate deposits were deposited in the heart, Liver and kidney and other parts, some cases also have urate deposition in the subcutaneous and gallbladder.

[0025] Referring to the method in Zhang Qingshui's paper (Zhang Qingshui. Isolation and identification of new renal pathogenic goose astroviruses and breeding of attenuated strains [D]. China Agricultural University, 2019.), the possible existence of the gosling samples was analyzed. Goose parvovirus (GPV), goose hemorrhagic polyoma virus (GHPV), duck reovirus (DRV), Tembusu virus (Tembusu) and goose astrovirus (GoAstV) and other viral genes ...

Embodiment 2

[0043] Example 2: Preparation of goose astrovirus inactivated vaccine

[0044] 1. Preparation of Inactivated Vaccines

[0045] Step 1, take the 15th-20th generation goose astrovirus N-GoAstV-190123 virus seed and dilute it with diluent, and dilute it to ELD 50 5×10 5.00 / mL, inoculate 8-10-day-old SPF goose embryos through the chorioallantoic membrane route, 0.2ml per embryo, and continue to incubate at 37°C after inoculation without turning the eggs;

[0046] Step 2: After inoculation of SPF goose embryos, light the eggs 3 times a day, discard the goose embryos that died before 24 hours, and take out the goose embryos that died after 24 hours at any time, until 72 hours, take out all the live embryos with the air chamber facing upwards. Stand upright and cool at 4°C for 12 hours;

[0047] Step 3: Take out the goose embryos that have been cooled for 12 hours. After surface disinfection, suck the goose embryo fluid. Mix the embryo fluid of every 20 goose embryos into a group...

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Abstract

The invention relates to a goose star virus N-GoAstV-190123 strain with strong virulence and good immune performance. The virus strain is prepared as an inactivated vaccine and can produce higher neutralizing antibodies after immunization, which can reach all the geese. Protection has important economic value for the goose industry.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, mainly relates to the production of vaccines, and in particular relates to a goose astrovirus and its application. Background technique: [0002] Astrovirus is a non-encapsulated, positive-strand RNA virus that can infect humans, dogs, cats, pigs, cattle, sheep, chickens, ducks and other animals. It is one of the main pathogens that cause diarrhea in animals, causing acute gastric Enteritis, duck fatal hepatitis, chicken nephritis, goose visceral gout and mammalian encephalitis and other different diseases. At present, astrovirus infection is widespread in the world and has become one of the important infectious diseases threatening human health and economic animal breeding. In recent years, a fatal infectious disease with gout as the main symptom has broken out in goose farms in Shandong, Jiangsu and Anhui. The disease mainly occurs in 5-20-day-old goslings. Infected goslings have depression, los...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/12A61P31/14C12R1/93
CPCC12N7/00A61K39/12A61P31/14C12N2770/12021C12N2770/12034A61K2039/5252A61K2039/552
Inventor 姚伦广冀君刘阳坤许鑫胡小敏
Owner NANYANG NORMAL UNIV
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