Delftia sp. for regulating quorum sensing quenching as well as separation method and application of Delftia sp.

A Delft bacteria and quorum sensing technology, applied in the field of microbial separation, can solve the problems of low expression of acylase, unsuitable for practical application, low efficiency, etc., to reduce operating costs, high-efficiency AHL degradation ability, and improve effluent efficiency effect

Pending Publication Date: 2021-06-11
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, most of the quenched bacteria that can produce acylases in the prior art have the disadvantages of low expression of acylases and low efficiency, and are not suitable for practical applications.

Method used

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  • Delftia sp. for regulating quorum sensing quenching as well as separation method and application of Delftia sp.
  • Delftia sp. for regulating quorum sensing quenching as well as separation method and application of Delftia sp.
  • Delftia sp. for regulating quorum sensing quenching as well as separation method and application of Delftia sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The present embodiment provides a Delftia JL5 strain that regulates quorum sensing quenching, and the isolation method includes the following steps:

[0049] (1) Take the activated sludge sample in the MBR, dilute it 10 times with ultra-pure water, inoculate it in LB medium with 5% (v / v), and cultivate it at 200r / min, 30℃ for 48h with constant temperature and shaking. The solution was transferred to the basic medium with 2mmol / L C6-HSL as the only carbon source with 2% (v / v) (the formula is as shown in Table 1, and the 1000× trace element formula in the formula is as shown in Table 2), At 200r / min, 30°C constant temperature shaking enrichment culture for 24h.

[0050] Table 1

[0051]

[0052] Table 2

[0053]

[0054] (2) Dilute the culture solution of step (1) 100 times with sterile water, spread it on an LB agar plate, incubate at a constant temperature of 30°C for 48 hours, and pick colonies of different shapes for isolation and purification.

[0055] (3) I...

Embodiment 2

[0061] Morphological observation is carried out to the bacterial strain that embodiment 1 screening obtains, it is carried out Gram's stain and observes under microscope, as figure 2 As shown, it can be seen from the figure that the colonies of the strains on the LB agar plate have neat edges, smooth surface, opaque light yellow colonies, and Gram staining is negative.

[0062] It was observed by scanning electron microscope, such as image 3 As shown in the figure, it can be seen that the strain is rod-shaped, the length of the bacteria is about 2.6-3.1 μm, and the width is about 0.6-0.8 μm.

Embodiment 3

[0064] The strain obtained by screening in Example 1 is subjected to a physiological and biochemical characteristic identification experiment, and the results show that: glucose oxidation fermentation test, citrate test, and catalase test are positive; M.R test, indole test, V.P test, starch hydrolysis test, Gelatin hydrolysis test and acetylmethylmethanol test were negative.

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Abstract

The invention relates to a Delftia sp. For regulating and controlling quorum sensing quenching and a separation method and application thereof, the Delftia sp. For regulating and controlling quorum sensing quenching is named as Delftia sp. JL5 strain, the preservation unit is China Center for Type Culture Collection, the preservation time is January 21, 2021, the preservation number is CCTCCM 2021139, and the address is Wuhan University, Wuhan, Hubei Province. The Delftiasp. is a bacterium for producing acylase, the enzyme expression quantity is very high, compared with rhodococcus BH4 which is most widely applied at present, the Delftia shows higher N-acyl homoserine lactone (AHL) degradation capacity, and when the Delftia is added into a membrane bioreactor (MBR) through the efficient AHL degradation capacity of the Delftia sp., a biological membrane can be inhibited from being attached to the membrane component to prevent and control membrane pollution and blockage, the water outlet efficiency is improved, and the operation cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganism separation, and in particular relates to a Delft bacteria capable of efficiently quenching N-acyl homoserine lactones and its isolation method and application, especially to a Delft bacteria capable of regulating quorum induction quenching And its separation method and application. Background technique [0002] Quorum sensing refers to the changes in the physiological and biochemical characteristics of microbial populations due to the increase in population density during their growth, showing characteristics that a small number of bacteria or a single bacteria do not have. The reason for this change is that when the microbial population density in the environment reaches a threshold value, the concentration of signal molecules also reaches a certain level. Through the signal transmission of related proteins including receptor proteins, the inducing or inhibiting signals are finally transmit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/12B01D65/02C12R1/01
CPCC12N1/20C12N1/02C02F3/1268B01D65/02C02F2303/14Y02W10/10
Inventor 徐芳芳廖家龙郑雪漫王文昭毕兆顺
Owner SHENZHEN UNIV
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