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A kind of tea tree endophytic bacteria with plant growth-promoting effect, bacterial agent and application thereof

A technology of endophytic bacteria and tea tree, which is applied in the field of microorganisms, can solve the problem of less Erwinia rhubarb and achieve the effects of improving nutrient composition and absorption, promoting growth, and promoting absorption

Active Publication Date: 2022-07-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on Erwinia rheum endogenous in plants, especially there is no relevant research on Erwinia rheum endogenous in tea trees and its application in promoting plant growth.

Method used

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  • A kind of tea tree endophytic bacteria with plant growth-promoting effect, bacterial agent and application thereof
  • A kind of tea tree endophytic bacteria with plant growth-promoting effect, bacterial agent and application thereof
  • A kind of tea tree endophytic bacteria with plant growth-promoting effect, bacterial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Isolation and purification of endophytic bacteria of tea tree

[0060] Tea leaves were picked from the mass-planted tea trees in Huangshan, Anhui, put into clean sampling bags (bottles), marked, and stored in a 4°C refrigerator for later use.

[0061] After the surface disinfection of the leaves of the group-planted tea trees in Huangshan is completed, the leaf samples are cut into pieces and placed in a sterilized mortar, and a small amount of sterile water is added for grinding; amount of sterile water, configured as a grinding fluid, and then diluted into 3 concentration gradients of 10 -1 , 10 -2 , 10 -3 . Next, the three concentration-gradient grinding liquids were applied to the solid medium of three specific cultured bacteria, LB medium, NA medium, and TSA medium, respectively. After plating, the plates were placed in a 28°C incubator for 3 days, and single colonies of different shapes were picked and purified by repeated streaking three times to ob...

Embodiment 2

[0062] Example 2 Screening of tea plant endophytic bacteria with plant growth-promoting effect

[0063] 1. Culture of strains and preparation of bacterial suspension:

[0064] The strains isolated and purified in Example 1 were inoculated into LB liquid medium, shaken for 8 to 12 hours at 28°C and 180 r / min, centrifuged to collect bacterial cells, washed with normal saline, and resuspended to prepare bacterial suspension.

[0065] 2. Screening of strains

[0066] Through the five plant growth promotion indicators of IAA production capacity, ACC deaminase activity, nitrogen fixation capacity, phosphorus dissolution capacity and siderophore production capacity, the isolated strains were identified for their growth-promoting effect, so as to screen out the plant growth-promoting capacity. of endophytic bacteria.

[0067] (1) IAA production ability test

[0068] Plant auxin (IAA) has a direct growth stimulating effect on plants.

[0069] Test procedure: Take 1 mL of the prepa...

Embodiment 3

[0081] Example 3 Identification and preservation of tea plant endophytic bacteria V4 with plant growth-promoting effect

[0082] 1. Molecular identification of tea plant endophytic bacteria V4

[0083] The genomic DNA of the tea plant endophytic bacteria V4 described in Example 2 was extracted using a bacterial genome extraction kit (Biomiga brand of Xiyue Bio), and the 16S rDNA sequence of the strain was amplified using bacterial universal primers. Among them, the base sequence of the bacterial universal primer is:

[0084] F16S-27F: 5'-AGAGTTTGATCCTGGCTCAG-3' (as shown in SEQ ID NO: 2);

[0085] R16S-1492R: 5'-CGGTTACCTTGTTACGACTTC-3' (shown as SEQ ID NO: 3).

[0086] The amplified product was sequenced by Universal Biosystems (Anhui) Co., Ltd. The length of the 16S rDNA sequence was 1443 bp, and its sequence was shown in SEQ ID NO: 1.

[0087] The determined sequence was analyzed by Blast software and the known nucleotide sequence in the NCBI database. The resu...

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Abstract

The invention belongs to the technical field of microorganisms, and in particular relates to a tea tree endophytic bacteria with plant growth-promoting effect, an inoculum and an application thereof. The tea tree endophyte is Erwinia rhapontici V4, and the strain was deposited in the China Center for Type Culture Collection on January 7, 2021, and the deposit number is CCTCC NO: M2021027. The tea plant endophytic bacteria have the ability of IAA production, ACC deaminase activity, nitrogen fixation ability, phosphorus dissolving ability and siderophore production ability, thereby improving soil nutrient composition and absorption status, promoting plants to absorb more nutrients, and effectively promoting plant growth. grow. In addition, the tea tree endophyte bacteria are easy to cultivate, have a fast reproduction speed, are green, organic and pollution-free, and can be widely used in the preparation of plant growth-promoting microbial agents or microbial fertilizers.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a tea tree endophytic bacteria with plant growth-promoting effect, an inoculum and an application thereof. Background technique [0002] At present, pesticides and chemical fertilizers are widely used in agriculture, which can promote the growth and high yield of plants. However, with the continuous use of chemical fertilizers, pesticide residues exist in soil and food, posing a threat to human health and the living environment. Therefore, the search for high-efficiency and healthy plant growth promoters has become a special focus of people. to promote the growth and development of plants. [0003] In the current research, the plant growth-promoting strains used are mainly isolated from plants or the environment itself, and applied to the soil as microbial fertilizers and inoculants, which are safe, adaptable and durable. Chinese invention patent application...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/18
CPCA01N63/20Y02A40/10
Inventor 邓威威贾慧艳陆梦倩郝昌洪李一凡席祖国
Owner ANHUI AGRICULTURAL UNIVERSITY
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