Malus hupehensis tea water extract and application thereof

A technology of extract and Begonia, applied in Begonia tea water extract and its application field, can solve the problems such as the influence of uric acid metabolism that has not been reported in domestic and foreign literature and the like

Pending Publication Date: 2021-06-18
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Begonia tea has a regulating effect on blood sugar and blood lipid metabolism, but its effect on uric acid metabolism has not been reported in domestic and foreign literature

Method used

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  • Malus hupehensis tea water extract and application thereof
  • Malus hupehensis tea water extract and application thereof
  • Malus hupehensis tea water extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of test drug of the present invention: preparation of sea otter tea extract: 500 g of sea otter tea leaves, boiled three times at 100°C, each time with 10 times of water, slightly boiled for 30 minutes, after suction filtration, combined filtrates, concentrated under reduced pressure to 1:2 (ie 1ml Equivalent to 2g of crabapple tea), vacuum-dried to obtain (the extract of crabapple tea contains phloridin 8%, trihydroxyphloridin 2%, and phloretin about 1.8%).

[0030] Substrate solution preparation: dissolve xanthine with 1mol / L sodium hydroxide, add 30mL of distilled water, adjust the pH to 7.5 with 1mol / L hydrochloric acid, and prepare a 1.5mmol / L stock solution.

[0031] Enzyme solution configuration: XOD was dissolved in PBS, and prepared into 0.4U XOD stock solution and stored at -80°C. Diluted into different gradients before use and stored in an ice-water bath.

[0032] Sample solution configuration: Phloridin, phloridin, and trihydroxyphloridin were a...

Embodiment 2

[0035] Effects of Begonia Tea Extract and Main Components on Blood Uric Acid in Rats with Hyperuricemia

[0036] Establishment of hyperuricemia rat model 90 SD rats were randomly divided into blank group, model group, allopurinol group (5 mg / kg) and low, medium and high dose groups (200, 300 , 400mg / kg), phloridin low, medium and high dose groups (20, 40, 60mg / kg), phloretin low, medium and high dose groups (5, 10, 20mg / kg), trihydroxy root bark Glycoside low, medium and high dose groups (5, 10, 20mg / kg) (low water extract of crabapple tea, phloridin, phloridin, and trihydroxyphloridin were all made into a uniform aqueous suspension with water, the same below.) , 6 in each group. Rats in the experimental group were intragastrically administered potassium oxonate 300 mg / kg + yeast extract 15 g / kg to replicate the hyperuricemia model, and administered twice a day. One hour after modeling, each experimental group was given the corresponding samples by intragastric administratio...

Embodiment 3

[0043] Effects of Begonia Tea Extract and Main Components on Gouty Rats

[0044] Gout rat model establishment:

[0045] 90 SD rats were randomly divided into blank group, model group, allopurinol group (5mg / kg) and crabapple tea water extract low, medium and high dose groups, phlorizin low, medium and high dose groups according to body weight, Phloretin low, medium and high dose groups, trihydroxyphloridin low, medium and high dose groups (dose as above), 6 rats in each group. The initial circumference of the right ankle joint was measured on the 12th day after administration. Rats in each group were fixed in supine position, the hair on the calf joint of the right hind limb was removed, disinfected, and after anesthesia, a No. 6 injection needle was used to puncture the rear side of the right deep joint of the rat, and was inserted from the back of the calf joint to the inner side of the tibialis tendon from a direction of 45°. 200 μL of 25 mg / ml sodium urate solution, and ...

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Abstract

The invention provides an application of a Malus hupehensis tea water extract in preparation of medicines for treating hyperuricemia and gout. The results show that the main components of the Malus hupehensis tea water extract can effectively reduce the blood uric acid and joint uric acid levels of rats and the liver xanthine oxidase activity (P is less than 0.05 or P is less than 0.01). The IC50 of phlorizin to xanthine oxidase is (355.2 + / -1.78) mu g / mL, and belongs to mixed inhibition in reversible competitive inhibition. The IC50 of phloretin is (123.4 + / -1.03) mu g / mL, and belongs to competitive reversible inhibition. The IC50 of the trihydroxy phlorizin is (2.02 + / -1.79) mu g / mL, and belongs to anti-competitive reversible inhibition. The phlorizin and the phloretin have a synergistic effect on inhibition of xanthine oxidase. The Malus hupehensis tea and the main components including phlorizin, phloretin and trihydroxy phlorizin can regulate uric acid metabolism by means of inhibiting xanthine oxidase activity and the like, and have prevention and treatment effects on hyperuricemia and gout.

Description

technical field [0001] The invention provides a crabapple tea extract and its application, in particular to the application of the crabapple tea extract in the preparation of medicine for treating gout. Background technique [0002] Uric acid is the end product of purine metabolism in the human body. Under a normal purine diet, when the uric acid value in the human body exceeds a certain concentration (men>420μmol / L, women>360μmol / L), hyperuricemia develops. Hyperuricemia caused by purine metabolism disorder and / or decreased uric acid excretion is directly related to the pathogenesis of gout. Gout is the second largest metabolic disease in my country, which can cause joint tissue damage and a variety of chronic diseases, seriously affecting human life and health. Therefore, it is of great significance to find foods and medicines that regulate metabolism, reduce blood uric acid, and prevent and treat gout. [0003] Xanthine oxidase (Xanthine oxidase, XOD) is a key en...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61K31/7034A61K31/12A61P19/06A23F3/34A61K127/00
CPCA61K36/73A61K31/7034A61K31/12A61P19/06A23F3/34A61K2236/331A61K2236/51A61K2300/00
Inventor 汪鋆植颜华玲王宇王丹阳张晓兰张宏岐苏香萍邓张双
Owner CHINA THREE GORGES UNIV
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