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Biomarker for diagnosing primary sicca syndrome and application of biomarker

A technology for Sjögren's syndrome and biomarkers, applied in disease diagnosis, biomaterial analysis, instruments, etc., can solve the problems of loss of IgG pro-inflammatory activity and anti-inflammatory activity.

Active Publication Date: 2021-06-22
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In fact, complete removal of polysaccharides leads to loss of IgG pro-inflammatory and anti-inflammatory activities

Method used

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  • Biomarker for diagnosing primary sicca syndrome and application of biomarker
  • Biomarker for diagnosing primary sicca syndrome and application of biomarker
  • Biomarker for diagnosing primary sicca syndrome and application of biomarker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Lectin microarray analysis of serum IgG glycosylation

[0032] Experimental specimens: This study included three groups of people: PSS group (40 cases of PSS patients), PBC group (50 cases of PBC disease controls), NC group (38 cases of healthy persons), see Table 1 for details. Among them, the diagnoses of PSS group and PBC group were in line with the diagnostic criteria of corresponding diseases. Fresh blood was collected from all the people enrolled in the group, and the serum was separated immediately and frozen at -80°C for later use.

[0033] Table 1 Basic information of the research objects in the PSS chip experiment

[0034] group Number of cases men and women age range average age PSS 40 1 / 9 31-68 48.52±9.73 PBC 50 2 / 23 34-75 52.30±10.13 healthy control group 38 3 / 35 35-70 45.60±7.64

[0035] A lectin microarray containing 56 lectins was used to detect the glycosylation status of experimental samples....

Embodiment 2

[0045] Example 2 Serum lectin imprint verification experiment

[0046] Experimental specimens and methods: In order to further clarify the reliability of the above-mentioned lectin microarray detection conclusions, the same batch of PSS specimens, PBC and NC specimens (12 cases each) and a new batch of specimens in the same group ( 16 cases each) were verified by lectin blot, and the basic information of the verified objects is shown in Table 4.

[0047] Table 4 Basic information of verification objects

[0048]

[0049]

[0050] Serum samples were diluted 1:100, mixed with sample buffer, boiled at 100°C for 5 minutes, performed SDS-PAGE electrophoresis in 10% precast gel, and then electrotransferred the protein in the precast gel to PVDF membrane. After the successfully transferred PVDF membrane was blocked, it was hybridized with cy3-labeled lectin, and finally the fluorescence signal was detected by a fluorescence imager. The intensity of the fluorescent signal is dir...

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Abstract

The invention relates to a biomarker for diagnosing primary sicca syndrome and application of the biomarker. According to the biomarker for diagnosing primary sicca syndrome and application of the biomarker of the invention, a lectin microarray containing 56 types of lectins is adopted to detect a glycan spectrum of specific binding of the serum IgG and the lectin of a patient with the primary sicca syndrome (PSS). The results show that LCA lectin binding glycan content is increased in the patient with primary biliary cholangitis (PBC) compared with that of a healthy person. As the LCA lectin is specific binding fucose, the expression of the fucose level in the patient withPSS is increased. A lectin immunoblotting verification result shows that the content of the LCA lectin binding glycan is still increased in the patient with the PSS. Therefore, the LCA lectin binding glycan level in the serum IgG can be used as a marker of the primary sicca syndrome (PSS).

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a biomarker for diagnosing primary Sjögren's syndrome and its application. Background technique [0002] Primary Sjogren's Syndrome (PSS) is a complex heterogeneous autoimmune disease characterized by a wide range of clinical manifestations, including Sjogren's syndrome caused by chronic inflammation of exocrine glands (mainly Lymphocytic infiltration of the lacrimal and salivary glands, leading to dry eyes and / or mouth), to systemic multi-organ involvement, which may evolve into non-Hodgkin lymphoma mucosa-associated lymphoid disease. Scientists have proposed various biomarkers for PSS diagnosis over the years. However, there is still an urgent need for new specific biomarkers that could improve the diagnosis of the disease and the management of PSS patients. Indeed, delays from symptom onset and PSS diagnosis remain common, and diagnosis still relies on invasiv...

Claims

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Application Information

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IPC IPC(8): G01N33/564
CPCG01N33/564G01N2800/7095G01N2333/4724
Inventor 胡朝军曾小莉李晞唐诗逸李斯亭李梦涛曾小峰
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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